Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)

Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)

Summary: Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in...

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Bibliographic Details
Main Authors: Manuela Sauter, Reinhard J. Sauter, Henry Nording, Marcus Olbrich, Frederic Emschermann, Harald F. Langer
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell biology
Cell culture
Cell isolation
Flow cytometry/Mass cytometry
Health sciences
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005445

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