Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation
IntroductionChimerism after orthotopic liver transplantation (OLT) has largely been investigated in intrahepatic cellular constituents. However, little is known about chimerism in the extrahepatic and large intrahepatic bile ducts. Our aim was to evaluate the presence and extent of chimerism after O...
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00693/full |
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| author | Iris E. M. de Jong Iris E. M. de Jong Michael E. Sutton Michael E. Sutton Marius C. van den Heuvel Annette S. H. Gouw Robert J. Porte |
| author_facet | Iris E. M. de Jong Iris E. M. de Jong Michael E. Sutton Michael E. Sutton Marius C. van den Heuvel Annette S. H. Gouw Robert J. Porte |
| author_sort | Iris E. M. de Jong |
| collection | DOAJ |
| description | IntroductionChimerism after orthotopic liver transplantation (OLT) has largely been investigated in intrahepatic cellular constituents. However, little is known about chimerism in the extrahepatic and large intrahepatic bile ducts. Our aim was to evaluate the presence and extent of chimerism after OLT in the peribiliary glands (PBG) and the luminal epithelium of the large donor bile ducts.MethodsFor this study, we examined six extrahepatic and large intrahepatic bile ducts from livers that were re-transplanted. In all cases there was a sex-mismatch between donor and recipient (female donor organ and male recipient), which allowed to discriminate between donor- and recipient-derived cells. Specimens from female to female transplants were used as negative controls and male to male transplants as positive controls. Fluorescence in situ hybridization (FISH) for Y and X chromosomes was performed and the percentage of XY positive cells was determined among biliary epithelial cells. Immunohistochemistry was used to correlate chimerism with histological features.ResultsCholangiocellular chimerism in all studied specimens ranged from 14 to 52%. The degree of chimerism was not associated with biliary damage. Marked chimerism was present at 5 days post-OLT. Ki-67-positivity was detected in 1–8% of the epithelial cells at the time of liver re-transplantation, and this correlated inversely with the degree of chimerism.ConclusionRecipient-derived cholangiocytes are present in the large bile ducts of the donor liver after OLT. The presence of chimerism in the large bile ducts suggests that recipient-derived cells may play a role in biliary regeneration following ischemia-induced injury during OLT. |
| first_indexed | 2024-12-11T16:11:04Z |
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| id | doaj.art-5a69b211a25d49c782d81d7b574d9328 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-11T16:11:04Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-5a69b211a25d49c782d81d7b574d93282022-12-22T00:59:04ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-08-01810.3389/fcell.2020.00693539416Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver TransplantationIris E. M. de Jong0Iris E. M. de Jong1Michael E. Sutton2Michael E. Sutton3Marius C. van den Heuvel4Annette S. H. Gouw5Robert J. Porte6Section of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsSurgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsSection of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsSurgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Pathology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Pathology, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsSection of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsIntroductionChimerism after orthotopic liver transplantation (OLT) has largely been investigated in intrahepatic cellular constituents. However, little is known about chimerism in the extrahepatic and large intrahepatic bile ducts. Our aim was to evaluate the presence and extent of chimerism after OLT in the peribiliary glands (PBG) and the luminal epithelium of the large donor bile ducts.MethodsFor this study, we examined six extrahepatic and large intrahepatic bile ducts from livers that were re-transplanted. In all cases there was a sex-mismatch between donor and recipient (female donor organ and male recipient), which allowed to discriminate between donor- and recipient-derived cells. Specimens from female to female transplants were used as negative controls and male to male transplants as positive controls. Fluorescence in situ hybridization (FISH) for Y and X chromosomes was performed and the percentage of XY positive cells was determined among biliary epithelial cells. Immunohistochemistry was used to correlate chimerism with histological features.ResultsCholangiocellular chimerism in all studied specimens ranged from 14 to 52%. The degree of chimerism was not associated with biliary damage. Marked chimerism was present at 5 days post-OLT. Ki-67-positivity was detected in 1–8% of the epithelial cells at the time of liver re-transplantation, and this correlated inversely with the degree of chimerism.ConclusionRecipient-derived cholangiocytes are present in the large bile ducts of the donor liver after OLT. The presence of chimerism in the large bile ducts suggests that recipient-derived cells may play a role in biliary regeneration following ischemia-induced injury during OLT.https://www.frontiersin.org/article/10.3389/fcell.2020.00693/fullfluorescence in situ hybridizationperibiliary glandsliver transplantationpost-transplant cholangiopathyregeneration |
| spellingShingle | Iris E. M. de Jong Iris E. M. de Jong Michael E. Sutton Michael E. Sutton Marius C. van den Heuvel Annette S. H. Gouw Robert J. Porte Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation Frontiers in Cell and Developmental Biology fluorescence in situ hybridization peribiliary glands liver transplantation post-transplant cholangiopathy regeneration |
| title | Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation |
| title_full | Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation |
| title_fullStr | Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation |
| title_full_unstemmed | Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation |
| title_short | Evidence for Recipient-Derived Cells in Peribiliary Glands and Biliary Epithelium of the Large Donor Bile Ducts After Liver Transplantation |
| title_sort | evidence for recipient derived cells in peribiliary glands and biliary epithelium of the large donor bile ducts after liver transplantation |
| topic | fluorescence in situ hybridization peribiliary glands liver transplantation post-transplant cholangiopathy regeneration |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00693/full |
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