Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines
Maize Research Institute Zemun Polje has a marker-assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Ga1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker-assisted develop...
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| Format: | Article |
| Language: | English |
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Serbian Association of Plant Breedings and Seed Producers
2023-01-01
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| Series: | Selekcija i Semenarstvo |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2023/0354-58812302017K.pdf |
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| author | Kostadinović Marija Ristić Danijela Božinović Sofija Đorđević-Melnik Olivera Ignjatović-Micić Dragana Vančetović Jelena |
| author_facet | Kostadinović Marija Ristić Danijela Božinović Sofija Đorđević-Melnik Olivera Ignjatović-Micić Dragana Vančetović Jelena |
| author_sort | Kostadinović Marija |
| collection | DOAJ |
| description | Maize Research Institute Zemun Polje has a marker-assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Ga1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker-assisted development of the white maize hybrid's parental lines with complete cross-incompatibility to foreign pollens. Ga1-S-tightly-linked molecular markers successfully identified heterozygous plants with a percentage of approximately 50% after all three backcrossings. Regarding the background selection, 69% of progenies had recurrent parent's genome (RPG) above the theoretical value for BC3 generation. Also, a few individuals even had 99%, the value theoretically achieved in the BC6 generation, showing that MABB made a genetic gain in RPG recovery. Foreground selection in this generation aimed to identify homozygous dominant individuals. Out of 264 BC3 F2 plants, 70 (26.5%) were dominant homozygotes, which is in accordance with the expected Mendelian ratio. Progenies of the BC3 F2 homozygous dominant plants were planted alternatively with yellow-kernel maize of the same maturity to check cross-incompatibility. Unfortunately, none of the dominant progenies had a 100% white kernel, most likely due to the presence of modifier genes that increase/decrease the effectiveness of pollen exclusion or that, alternatively, modifiers are lost during the backcrossing. It could be expected that successfully employed functional markers (developed from the sequence polymorphisms present within a functional gene(s) associated with phenotypic trait variations) would outbalance the noted impediments and enhance MABB efficiency to transfer the desired gene(s) controlling simple or complex trait(s) into cultivated varieties. |
| first_indexed | 2024-03-08T04:09:33Z |
| format | Article |
| id | doaj.art-5a6b8e0041bd46c0a2b44688384a57d3 |
| institution | Directory Open Access Journal |
| issn | 0354-5881 2406-209X |
| language | English |
| last_indexed | 2024-03-08T04:09:33Z |
| publishDate | 2023-01-01 |
| publisher | Serbian Association of Plant Breedings and Seed Producers |
| record_format | Article |
| series | Selekcija i Semenarstvo |
| spelling | doaj.art-5a6b8e0041bd46c0a2b44688384a57d32024-02-08T22:53:20ZengSerbian Association of Plant Breedings and Seed ProducersSelekcija i Semenarstvo0354-58812406-209X2023-01-01292172410.5937/SelSem2302017K0354-58812302017KMarker-assisted Ga1-S incorporation into white maize hybrid's parental linesKostadinović Marija0https://orcid.org/0000-0001-7267-0730Ristić Danijela1https://orcid.org/0000-0002-3145-4158Božinović Sofija2https://orcid.org/0000-0002-1966-1656Đorđević-Melnik Olivera3https://orcid.org/0000-0003-2046-3275Ignjatović-Micić Dragana4https://orcid.org/0000-0003-3584-3887Vančetović Jelena5https://orcid.org/0000-0002-8743-0454Maize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje, Belgrade, SerbiaMaize Research Institute Zemun Polje has a marker-assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Ga1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker-assisted development of the white maize hybrid's parental lines with complete cross-incompatibility to foreign pollens. Ga1-S-tightly-linked molecular markers successfully identified heterozygous plants with a percentage of approximately 50% after all three backcrossings. Regarding the background selection, 69% of progenies had recurrent parent's genome (RPG) above the theoretical value for BC3 generation. Also, a few individuals even had 99%, the value theoretically achieved in the BC6 generation, showing that MABB made a genetic gain in RPG recovery. Foreground selection in this generation aimed to identify homozygous dominant individuals. Out of 264 BC3 F2 plants, 70 (26.5%) were dominant homozygotes, which is in accordance with the expected Mendelian ratio. Progenies of the BC3 F2 homozygous dominant plants were planted alternatively with yellow-kernel maize of the same maturity to check cross-incompatibility. Unfortunately, none of the dominant progenies had a 100% white kernel, most likely due to the presence of modifier genes that increase/decrease the effectiveness of pollen exclusion or that, alternatively, modifiers are lost during the backcrossing. It could be expected that successfully employed functional markers (developed from the sequence polymorphisms present within a functional gene(s) associated with phenotypic trait variations) would outbalance the noted impediments and enhance MABB efficiency to transfer the desired gene(s) controlling simple or complex trait(s) into cultivated varieties.https://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2023/0354-58812302017K.pdfbackground selectionforeground selectionga1-smaizemarker-assisted backcross breeding |
| spellingShingle | Kostadinović Marija Ristić Danijela Božinović Sofija Đorđević-Melnik Olivera Ignjatović-Micić Dragana Vančetović Jelena Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines Selekcija i Semenarstvo background selection foreground selection ga1-s maize marker-assisted backcross breeding |
| title | Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines |
| title_full | Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines |
| title_fullStr | Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines |
| title_full_unstemmed | Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines |
| title_short | Marker-assisted Ga1-S incorporation into white maize hybrid's parental lines |
| title_sort | marker assisted ga1 s incorporation into white maize hybrid s parental lines |
| topic | background selection foreground selection ga1-s maize marker-assisted backcross breeding |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2023/0354-58812302017K.pdf |
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