Screening of potential donors for anaerobic stress tolerance during germination in rice
The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1261101/full |
| _version_ | 1797629491404603392 |
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| author | Lupakisyo Mwakyusa Lupakisyo Mwakyusa Maria Cristina Heredia Newton Lwiyiso Kilasi Richard R. Madege Max Herzog Shalabh Dixit |
| author_facet | Lupakisyo Mwakyusa Lupakisyo Mwakyusa Maria Cristina Heredia Newton Lwiyiso Kilasi Richard R. Madege Max Herzog Shalabh Dixit |
| author_sort | Lupakisyo Mwakyusa |
| collection | DOAJ |
| description | The rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas or uneven fields, limit germination. Rice possesses the unique ability of anaerobic germination, enabling it to sprout and emerge in oxygen-deprived conditions. Understanding rice’s response to anaerobic stress during germination is crucial for resilience breeding. Africa, although relying on direct seeding, has made limited progress in addressing flooding during germination compared to Asia. Anaerobic stress tolerance ensures successful crop emergence even in oxygen-limited environments and can help suppress weeds, a significant challenge in direct-seeded rice cultivation. This study aims to contribute by screening for potential rice donors exhibiting anaerobic stress tolerance. We screened 200 rice genotypes at Sokoine University of Agriculture (SUA) in Morogoro, Tanzania, primarily focusing on landraces with untapped potential. Using an alpha lattice design, we conducted two anaerobic experiments in September and October 2022, adding 7 cm of standing water immediately after dry seeding for flooded and maintaining a 2 cm water level after germination in the control for duration of 21 days. We identified potential donors based on selection index computed from genomic estimated breeding values (GEBVs) using eight variables: germination at 14 DAS, germination at 21 DAS, seedling height at 14 DAS, seedling height at 21 DAS, shoot dry matter at 21 DAS, root dry matter at 21 DAS, culm diameter at 21 DAS, and root length at 21DAS. Ten genotypes emerged as the most promising, exhibiting at least 70% germination in floodwater at 21 DAS and greater selection indices. These genotypes were like: Afaa Mwanza 1/159, Rojomena 271/10, Kubwa Jinga, Wahiwahi, Magongo ya Wayungu, Mpaka wa Bibi, Mwangaza, Tarabinzona, IB126-Bug 2013A, and Kanamalia with respective percentages of 75, 74, 71, 86, 75, 80, 71, 80, 70, and 73. These findings contribute to global efforts to mitigate the impacts of flooding during germination. These donors, will be potential to enrich the gene pool for anaerobic germination, providing valuable resources for breeding for flooding tolerance. |
| first_indexed | 2024-03-11T10:54:04Z |
| format | Article |
| id | doaj.art-5a6daba2036d464d8dca7d1650cab63b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T10:54:04Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5a6daba2036d464d8dca7d1650cab63b2023-11-13T11:10:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12611011261101Screening of potential donors for anaerobic stress tolerance during germination in riceLupakisyo Mwakyusa0Lupakisyo Mwakyusa1Maria Cristina Heredia2Newton Lwiyiso Kilasi3Richard R. Madege4Max Herzog5Shalabh Dixit6Department of Crop Science and Horticulture, College of Agriculture, Sokoine University of Agriculture, Morogoro, TanzaniaDepartment of Research and Innovation, Tanzania Agricultural Research Institute, Kigoma, TanzaniaRice Breeding Innovations Department, International Rice Research Institute, Los Baños, Laguna, PhilippinesDepartment of Crop Science and Horticulture, College of Agriculture, Sokoine University of Agriculture, Morogoro, TanzaniaDepartment of Crop Science and Horticulture, College of Agriculture, Sokoine University of Agriculture, Morogoro, TanzaniaDepartment of Biology, University of Copenhagen, Copenhagen, DenmarkRice Breeding Innovations Department, International Rice Research Institute, Los Baños, Laguna, PhilippinesThe rising cost of transplanting rice has made direct seeding an affordable alternative for rice establishment, particularly in Africa. However, direct seeding, while cost-effective, faces crop establishment challenges due to flooding. Uncontrolled water, driven by erratic rains in low-lying areas or uneven fields, limit germination. Rice possesses the unique ability of anaerobic germination, enabling it to sprout and emerge in oxygen-deprived conditions. Understanding rice’s response to anaerobic stress during germination is crucial for resilience breeding. Africa, although relying on direct seeding, has made limited progress in addressing flooding during germination compared to Asia. Anaerobic stress tolerance ensures successful crop emergence even in oxygen-limited environments and can help suppress weeds, a significant challenge in direct-seeded rice cultivation. This study aims to contribute by screening for potential rice donors exhibiting anaerobic stress tolerance. We screened 200 rice genotypes at Sokoine University of Agriculture (SUA) in Morogoro, Tanzania, primarily focusing on landraces with untapped potential. Using an alpha lattice design, we conducted two anaerobic experiments in September and October 2022, adding 7 cm of standing water immediately after dry seeding for flooded and maintaining a 2 cm water level after germination in the control for duration of 21 days. We identified potential donors based on selection index computed from genomic estimated breeding values (GEBVs) using eight variables: germination at 14 DAS, germination at 21 DAS, seedling height at 14 DAS, seedling height at 21 DAS, shoot dry matter at 21 DAS, root dry matter at 21 DAS, culm diameter at 21 DAS, and root length at 21DAS. Ten genotypes emerged as the most promising, exhibiting at least 70% germination in floodwater at 21 DAS and greater selection indices. These genotypes were like: Afaa Mwanza 1/159, Rojomena 271/10, Kubwa Jinga, Wahiwahi, Magongo ya Wayungu, Mpaka wa Bibi, Mwangaza, Tarabinzona, IB126-Bug 2013A, and Kanamalia with respective percentages of 75, 74, 71, 86, 75, 80, 71, 80, 70, and 73. These findings contribute to global efforts to mitigate the impacts of flooding during germination. These donors, will be potential to enrich the gene pool for anaerobic germination, providing valuable resources for breeding for flooding tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2023.1261101/fullfloodingricegerminationflood tolerancegenotypes |
| spellingShingle | Lupakisyo Mwakyusa Lupakisyo Mwakyusa Maria Cristina Heredia Newton Lwiyiso Kilasi Richard R. Madege Max Herzog Shalabh Dixit Screening of potential donors for anaerobic stress tolerance during germination in rice Frontiers in Plant Science flooding rice germination flood tolerance genotypes |
| title | Screening of potential donors for anaerobic stress tolerance during germination in rice |
| title_full | Screening of potential donors for anaerobic stress tolerance during germination in rice |
| title_fullStr | Screening of potential donors for anaerobic stress tolerance during germination in rice |
| title_full_unstemmed | Screening of potential donors for anaerobic stress tolerance during germination in rice |
| title_short | Screening of potential donors for anaerobic stress tolerance during germination in rice |
| title_sort | screening of potential donors for anaerobic stress tolerance during germination in rice |
| topic | flooding rice germination flood tolerance genotypes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1261101/full |
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