microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells
Abstract Cancer‐associated fibroblasts (CAFs) play a critical role in supporting tumour cells in all aspects of cancer development, such as cell proliferation, migration and angiogenesis. MicroRNAs (miRNAs) as regulatory molecules regulate the genes contributing to cell growth, differentiation, migr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-09-01
|
| Series: | Clinical and Translational Discovery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ctd2.132 |
| _version_ | 1811190719957696512 |
|---|---|
| author | Nasim Shenavar Laleh Shariati Mohammad Reza Hakimian Pooyan Makvandi Shaghayegh Haghjooy Javanmard |
| author_facet | Nasim Shenavar Laleh Shariati Mohammad Reza Hakimian Pooyan Makvandi Shaghayegh Haghjooy Javanmard |
| author_sort | Nasim Shenavar |
| collection | DOAJ |
| description | Abstract Cancer‐associated fibroblasts (CAFs) play a critical role in supporting tumour cells in all aspects of cancer development, such as cell proliferation, migration and angiogenesis. MicroRNAs (miRNAs) as regulatory molecules regulate the genes contributing to cell growth, differentiation, migration and apoptosis. According to the literature, miR‐200c, as a tumour suppressor, has low expression levels in CAFs. In this investigation, the effect of miR‐200c overexpression was evaluated on proliferation, migration and angiogenesis of triple‐negative breast cancer (TNBC) cells. The fibroblasts were isolated from normal and cancerous breast tissue. MiR‐200c expression was assessed using real‐time polymerase chain reaction in cancer‐associated and normal fibroblasts. Then, the effect of miR‐200c transfection on proliferation, migration and angiogenesis of TNBC cells was evaluated. Our results confirm that in the presence of miR‐200c transfected fibroblasts, the proliferation, migration and angiogenesis of cancer cells significantly decreased. This effect could be attributed to the reduction of growth factors provided by cancer‐associated fibroblasts after miRNA dysregulation. These results propose that miR‐200c acts as an effective tumour suppressor in many aspects of TNBC development and can be considered a potential therapeutic tool for breast cancer in the next generation of pharmaceutics. |
| first_indexed | 2024-04-11T14:53:54Z |
| format | Article |
| id | doaj.art-5a6f2a99aaa14e6e8d82857c5daecee2 |
| institution | Directory Open Access Journal |
| issn | 2768-0622 |
| language | English |
| last_indexed | 2024-04-11T14:53:54Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Discovery |
| spelling | doaj.art-5a6f2a99aaa14e6e8d82857c5daecee22022-12-22T04:17:19ZengWileyClinical and Translational Discovery2768-06222022-09-0123n/an/a10.1002/ctd2.132microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cellsNasim Shenavar0Laleh Shariati1Mohammad Reza Hakimian2Pooyan Makvandi3Shaghayegh Haghjooy Javanmard4Applied Physiology Research Center Isfahan Cardiovascular Research Institute Isfahan University of Medical Sciences Isfahan IranDepartment of Biomaterials Nanotechnology and Tissue Engineering School of Advanced Technologies in Medicine Isfahan University of Medical Sciences Isfahan IranCancer Prevention Research Center Omid Hospital Isfahan University of Medical Sciences Isfahan IranIstituto Italiano di Tecnologia Centre for Materials Interface viale Rinaldo Piaggio 34, 56025 Pontedera Pisa ItalyApplied Physiology Research Center Isfahan Cardiovascular Research Institute Isfahan University of Medical Sciences Isfahan IranAbstract Cancer‐associated fibroblasts (CAFs) play a critical role in supporting tumour cells in all aspects of cancer development, such as cell proliferation, migration and angiogenesis. MicroRNAs (miRNAs) as regulatory molecules regulate the genes contributing to cell growth, differentiation, migration and apoptosis. According to the literature, miR‐200c, as a tumour suppressor, has low expression levels in CAFs. In this investigation, the effect of miR‐200c overexpression was evaluated on proliferation, migration and angiogenesis of triple‐negative breast cancer (TNBC) cells. The fibroblasts were isolated from normal and cancerous breast tissue. MiR‐200c expression was assessed using real‐time polymerase chain reaction in cancer‐associated and normal fibroblasts. Then, the effect of miR‐200c transfection on proliferation, migration and angiogenesis of TNBC cells was evaluated. Our results confirm that in the presence of miR‐200c transfected fibroblasts, the proliferation, migration and angiogenesis of cancer cells significantly decreased. This effect could be attributed to the reduction of growth factors provided by cancer‐associated fibroblasts after miRNA dysregulation. These results propose that miR‐200c acts as an effective tumour suppressor in many aspects of TNBC development and can be considered a potential therapeutic tool for breast cancer in the next generation of pharmaceutics.https://doi.org/10.1002/ctd2.132angiogenesisbreast cancerfibroblastmicroenvironmentmiR‐200cmiRNA |
| spellingShingle | Nasim Shenavar Laleh Shariati Mohammad Reza Hakimian Pooyan Makvandi Shaghayegh Haghjooy Javanmard microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells Clinical and Translational Discovery angiogenesis breast cancer fibroblast microenvironment miR‐200c miRNA |
| title | microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells |
| title_full | microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells |
| title_fullStr | microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells |
| title_full_unstemmed | microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells |
| title_short | microRNA‐200c overexpression in cancer‐associated fibroblasts reduces the invasive properties of breast tumour cells |
| title_sort | microrna 200c overexpression in cancer associated fibroblasts reduces the invasive properties of breast tumour cells |
| topic | angiogenesis breast cancer fibroblast microenvironment miR‐200c miRNA |
| url | https://doi.org/10.1002/ctd2.132 |
| work_keys_str_mv | AT nasimshenavar microrna200coverexpressionincancerassociatedfibroblastsreducestheinvasivepropertiesofbreasttumourcells AT lalehshariati microrna200coverexpressionincancerassociatedfibroblastsreducestheinvasivepropertiesofbreasttumourcells AT mohammadrezahakimian microrna200coverexpressionincancerassociatedfibroblastsreducestheinvasivepropertiesofbreasttumourcells AT pooyanmakvandi microrna200coverexpressionincancerassociatedfibroblastsreducestheinvasivepropertiesofbreasttumourcells AT shaghayeghhaghjooyjavanmard microrna200coverexpressionincancerassociatedfibroblastsreducestheinvasivepropertiesofbreasttumourcells |