Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin
Whey proteins, which are mainly composed of b-lactoglobulin (beta-lg) and alpha-lactalbumin (alpha-la), account for about 20% of the proteins of bovine milk. In this study we investigated the effect of pH, dry matter content, concentration factor, heat treatment and centrifugation on the separation...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Scientific Agricultural Society of Finland
2008-12-01
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| Series: | Agricultural and Food Science |
| Online Access: | https://journal.fi/afs/article/view/5557 |
| _version_ | 1818344371698794496 |
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| author | T. TUPASELA P. KOSKELA E. PAHKALA |
| author_facet | T. TUPASELA P. KOSKELA E. PAHKALA |
| author_sort | T. TUPASELA |
| collection | DOAJ |
| description | Whey proteins, which are mainly composed of b-lactoglobulin (beta-lg) and alpha-lactalbumin (alpha-la), account for about 20% of the proteins of bovine milk. In this study we investigated the effect of pH, dry matter content, concentration factor, heat treatment and centrifugation on the separation of alpha-la from beta-lg using clarified whey as raw material. alpha-La precipitation was highest, 23.3%, when the dry matter content ranged from 5.8% to 25.7%. The optimum pH of alpha-la precipitation depended on the dry matter content. The separation efficiency increased when the concentration factor and heat treatment time at 55°C increased. A longer centrifugation time and higher separation speed did not have a marked effect on the separation efficiency. Separation was more efficient with a higher centrifugation speed at concentration levels 30 X and 60 X. The separation efficiency did not improve when the temperature was raised from 55°C to 65°C but it was better at a concentration level 120 X than at 60 X and 30 X, and also at concentration level 60 X than with 30 X.; |
| first_indexed | 2024-12-13T16:45:25Z |
| format | Article |
| id | doaj.art-5a70020b05514132a44911301b9f1aa4 |
| institution | Directory Open Access Journal |
| issn | 1459-6067 1795-1895 |
| language | English |
| last_indexed | 2024-12-13T16:45:25Z |
| publishDate | 2008-12-01 |
| publisher | Scientific Agricultural Society of Finland |
| record_format | Article |
| series | Agricultural and Food Science |
| spelling | doaj.art-5a70020b05514132a44911301b9f1aa42022-12-21T23:38:10ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18952008-12-0162Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulinT. TUPASELAP. KOSKELAE. PAHKALAWhey proteins, which are mainly composed of b-lactoglobulin (beta-lg) and alpha-lactalbumin (alpha-la), account for about 20% of the proteins of bovine milk. In this study we investigated the effect of pH, dry matter content, concentration factor, heat treatment and centrifugation on the separation of alpha-la from beta-lg using clarified whey as raw material. alpha-La precipitation was highest, 23.3%, when the dry matter content ranged from 5.8% to 25.7%. The optimum pH of alpha-la precipitation depended on the dry matter content. The separation efficiency increased when the concentration factor and heat treatment time at 55°C increased. A longer centrifugation time and higher separation speed did not have a marked effect on the separation efficiency. Separation was more efficient with a higher centrifugation speed at concentration levels 30 X and 60 X. The separation efficiency did not improve when the temperature was raised from 55°C to 65°C but it was better at a concentration level 120 X than at 60 X and 30 X, and also at concentration level 60 X than with 30 X.;https://journal.fi/afs/article/view/5557 |
| spellingShingle | T. TUPASELA P. KOSKELA E. PAHKALA Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin Agricultural and Food Science |
| title | Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin |
| title_full | Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin |
| title_fullStr | Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin |
| title_full_unstemmed | Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin |
| title_short | Optimization of centrifugal separation of alpha-lactalbumin and beta-lactoglobulin |
| title_sort | optimization of centrifugal separation of alpha lactalbumin and beta lactoglobulin |
| url | https://journal.fi/afs/article/view/5557 |
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