<i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation
<i>Lactobacillus rhamnosus</i> (LBS) is a well-documented probiotic strain in oncology and has a pivotal role in clinical applications. Here, we have investigated the protective effect of <i>Lactobacillus rhamnosus</i> on intestinal mucositis induced by cisplatin (CP) and exp...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/11/1340 |
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| author | Duaa M. Alsholi Ghazi Suleiman Yacoub Ata Ur Rehman Hidayat Ullah Asif Iqbal Khan Ting Deng Nimra Zafar Siddiqui Yamina Alioui Nabeel Ahmed Farooqui Maroua Elkharti Yanxia Li Liang Wang Yi Xin |
| author_facet | Duaa M. Alsholi Ghazi Suleiman Yacoub Ata Ur Rehman Hidayat Ullah Asif Iqbal Khan Ting Deng Nimra Zafar Siddiqui Yamina Alioui Nabeel Ahmed Farooqui Maroua Elkharti Yanxia Li Liang Wang Yi Xin |
| author_sort | Duaa M. Alsholi |
| collection | DOAJ |
| description | <i>Lactobacillus rhamnosus</i> (LBS) is a well-documented probiotic strain in oncology and has a pivotal role in clinical applications. Here, we have investigated the protective effect of <i>Lactobacillus rhamnosus</i> on intestinal mucositis induced by cisplatin (CP) and explored the underlying mechanisms targeting inflammatory proteins, as well as the histological changes in the intestinal tissue of mice, in addition, the bacterial strains that may be related to the health-enhancing properties. BALB/c mice were pre-treated with or without LBS via oral gavage, followed by mucositis induction with cisplatin. Our results revealed that the LBS-treated groups significantly attenuated proinflammatory cytokine levels (IL-1β, IL-6, and TNF-α) compared to the CP group. Furthermore, LBS mitigated the damaged tight junction integrity caused by CP via up-regulating the levels of claudin, occludin, ZO-1, and mucin-2 protein (MUC-2). Finally, the 16S rRNA fecal microbiome genomic analysis showed that LBS administration enhanced the growth of beneficial bacteria, i.e., <i>Firmicutes and Lachnospiraceae,</i> while the relative abundance of the opportunistic bacteria <i>Bacteroides</i> and <i>Proteobacteria</i> decreased. Collectively, LBS was found to beneficially modulate microbial composition structure and functions and enrich the ecological diversity in the gut. |
| first_indexed | 2024-03-09T16:32:16Z |
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| id | doaj.art-5a71c6607a874021a075eaaf2610e24a |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T16:32:16Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-5a71c6607a874021a075eaaf2610e24a2023-11-24T14:59:49ZengMDPI AGPathogens2076-08172023-11-011211134010.3390/pathogens12111340<i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal InflammationDuaa M. Alsholi0Ghazi Suleiman Yacoub1Ata Ur Rehman2Hidayat Ullah3Asif Iqbal Khan4Ting Deng5Nimra Zafar Siddiqui6Yamina Alioui7Nabeel Ahmed Farooqui8Maroua Elkharti9Yanxia Li10Liang Wang11Yi Xin12Department of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Dermatology, The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Lu, Dalian 116011, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, ChinaDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaStem Cell Clinical Research Center, National Joint Engineering Laboratory, Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaDepartment of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, China<i>Lactobacillus rhamnosus</i> (LBS) is a well-documented probiotic strain in oncology and has a pivotal role in clinical applications. Here, we have investigated the protective effect of <i>Lactobacillus rhamnosus</i> on intestinal mucositis induced by cisplatin (CP) and explored the underlying mechanisms targeting inflammatory proteins, as well as the histological changes in the intestinal tissue of mice, in addition, the bacterial strains that may be related to the health-enhancing properties. BALB/c mice were pre-treated with or without LBS via oral gavage, followed by mucositis induction with cisplatin. Our results revealed that the LBS-treated groups significantly attenuated proinflammatory cytokine levels (IL-1β, IL-6, and TNF-α) compared to the CP group. Furthermore, LBS mitigated the damaged tight junction integrity caused by CP via up-regulating the levels of claudin, occludin, ZO-1, and mucin-2 protein (MUC-2). Finally, the 16S rRNA fecal microbiome genomic analysis showed that LBS administration enhanced the growth of beneficial bacteria, i.e., <i>Firmicutes and Lachnospiraceae,</i> while the relative abundance of the opportunistic bacteria <i>Bacteroides</i> and <i>Proteobacteria</i> decreased. Collectively, LBS was found to beneficially modulate microbial composition structure and functions and enrich the ecological diversity in the gut.https://www.mdpi.com/2076-0817/12/11/1340<i>Lactobacillus rhamnosus</i>probioticintestinal mucositiscisplatinproinflammatory cytokines16S rRNA fecal microbiome |
| spellingShingle | Duaa M. Alsholi Ghazi Suleiman Yacoub Ata Ur Rehman Hidayat Ullah Asif Iqbal Khan Ting Deng Nimra Zafar Siddiqui Yamina Alioui Nabeel Ahmed Farooqui Maroua Elkharti Yanxia Li Liang Wang Yi Xin <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation Pathogens <i>Lactobacillus rhamnosus</i> probiotic intestinal mucositis cisplatin proinflammatory cytokines 16S rRNA fecal microbiome |
| title | <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation |
| title_full | <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation |
| title_fullStr | <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation |
| title_full_unstemmed | <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation |
| title_short | <i>Lactobacillus rhamnosus</i> Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation |
| title_sort | i lactobacillus rhamnosus i attenuates cisplatin induced intestinal mucositis in mice via modulating the gut microbiota and improving intestinal inflammation |
| topic | <i>Lactobacillus rhamnosus</i> probiotic intestinal mucositis cisplatin proinflammatory cytokines 16S rRNA fecal microbiome |
| url | https://www.mdpi.com/2076-0817/12/11/1340 |
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