Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells
Esculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure. In this study, human embryonic kidney 293 (HEK293) cells were treated with tert-b...
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MDPI AG
2022-11-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/12/407 |
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| author | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim |
| author_facet | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim |
| author_sort | Woo Kwon Jung |
| collection | DOAJ |
| description | Esculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure. In this study, human embryonic kidney 293 (HEK293) cells were treated with tert-butyl hydroperoxide (t-BHP) to determine the antioxidant effects of esculetin. HEK293 cells were treated with t-BHP to validate changes in cell viability, ROS production, and apoptosis, and then treated with esculetin to evaluate the changes. Changes in mRNA and protein levels were analyzed using a proteome kit, PCR, and Western blotting. Esculetin improved HEK293 cell viability and reduced apoptosis caused by t-BHP-induced oxidative stress. At the mRNA and protein levels, esculetin decreased pro-apoptotic factor expression as well as increased anti-apoptotic factor expression. The antioxidant efficacy of esculetin was validated when it inhibited the apoptosis caused by t-BHP-induced oxidative stress in HEK293 cells. |
| first_indexed | 2024-03-09T17:11:22Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T17:11:22Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-5a72a0fba7084845bc5264094453f41a2023-11-24T14:03:31ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-11-0144125986599410.3390/cimb44120407Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 CellsWoo Kwon Jung0Su-Bin Park1Hwa Young Yu2Yong Hwan Kim3Junghyun Kim4Department of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaEsculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure. In this study, human embryonic kidney 293 (HEK293) cells were treated with tert-butyl hydroperoxide (t-BHP) to determine the antioxidant effects of esculetin. HEK293 cells were treated with t-BHP to validate changes in cell viability, ROS production, and apoptosis, and then treated with esculetin to evaluate the changes. Changes in mRNA and protein levels were analyzed using a proteome kit, PCR, and Western blotting. Esculetin improved HEK293 cell viability and reduced apoptosis caused by t-BHP-induced oxidative stress. At the mRNA and protein levels, esculetin decreased pro-apoptotic factor expression as well as increased anti-apoptotic factor expression. The antioxidant efficacy of esculetin was validated when it inhibited the apoptosis caused by t-BHP-induced oxidative stress in HEK293 cells.https://www.mdpi.com/1467-3045/44/12/407esculetintert-butyl hydroperoxidereactive oxygen specieskidney dysfunction |
| spellingShingle | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells Current Issues in Molecular Biology esculetin tert-butyl hydroperoxide reactive oxygen species kidney dysfunction |
| title | Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells |
| title_full | Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells |
| title_fullStr | Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells |
| title_full_unstemmed | Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells |
| title_short | Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells |
| title_sort | antioxidant efficacy of esculetin against tert butyl hydroperoxide induced oxidative stress in hek293 cells |
| topic | esculetin tert-butyl hydroperoxide reactive oxygen species kidney dysfunction |
| url | https://www.mdpi.com/1467-3045/44/12/407 |
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