Low-cost conditioned place preference setup including video recording and analysis of behaviour
The conditioned place preference (CPP) paradigm is widely used in rodent research to test the rewarding and aversive properties of different stimuli. Despite its relative simplicity, commercially available CPP systems are often costly. Here we describe the construction of a CPP setup and a behaviora...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120301187 |
| _version_ | 1818720182783180800 |
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| author | Philip Vassilev Moataz Salim Christina Popescu Cecilia Flores Giovanni Hernandez |
| author_facet | Philip Vassilev Moataz Salim Christina Popescu Cecilia Flores Giovanni Hernandez |
| author_sort | Philip Vassilev |
| collection | DOAJ |
| description | The conditioned place preference (CPP) paradigm is widely used in rodent research to test the rewarding and aversive properties of different stimuli. Despite its relative simplicity, commercially available CPP systems are often costly. Here we describe the construction of a CPP setup and a behavioral data analysis pipeline incorporating:• a CPP box which can be built in a single day by using widely available and affordable materials.• an open source computer system for data acquisition (based on Raspberry Pi).• a freely available behavioural analysis software.The behavioural analysis allows for measurement of both locomotor activity and time spent in a zone of interest. Including all components, our setup costs ~1/10 of the cost of the least expensive commercially available CPP boxes alone (not including data acquisition and analysis). We validated the setup by showing that a 4 mg/kg dose of amphetamine increases locomotor activity acutely in adolescent male mice and induces conditioned preference for the drug-paired compartment in the CPP test. |
| first_indexed | 2024-12-17T20:18:47Z |
| format | Article |
| id | doaj.art-5a74ad854ba345ba92d04a6038257403 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-17T20:18:47Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-5a74ad854ba345ba92d04a60382574032022-12-21T21:34:00ZengElsevierMethodsX2215-01612020-01-017100899Low-cost conditioned place preference setup including video recording and analysis of behaviourPhilip Vassilev0Moataz Salim1Christina Popescu2Cecilia Flores3Giovanni Hernandez4Corresponding author.; Department of Psychiatry, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montreal, Quebec, H4H1R3, CanadaDepartment of Psychiatry, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montreal, Quebec, H4H1R3, CanadaDepartment of Psychiatry, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montreal, Quebec, H4H1R3, CanadaDepartment of Psychiatry, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montreal, Quebec, H4H1R3, CanadaDepartment of Psychiatry, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montreal, Quebec, H4H1R3, CanadaThe conditioned place preference (CPP) paradigm is widely used in rodent research to test the rewarding and aversive properties of different stimuli. Despite its relative simplicity, commercially available CPP systems are often costly. Here we describe the construction of a CPP setup and a behavioral data analysis pipeline incorporating:• a CPP box which can be built in a single day by using widely available and affordable materials.• an open source computer system for data acquisition (based on Raspberry Pi).• a freely available behavioural analysis software.The behavioural analysis allows for measurement of both locomotor activity and time spent in a zone of interest. Including all components, our setup costs ~1/10 of the cost of the least expensive commercially available CPP boxes alone (not including data acquisition and analysis). We validated the setup by showing that a 4 mg/kg dose of amphetamine increases locomotor activity acutely in adolescent male mice and induces conditioned preference for the drug-paired compartment in the CPP test.http://www.sciencedirect.com/science/article/pii/S2215016120301187CPPanimal trackingmodels of reinforcementRaspberry Pi |
| spellingShingle | Philip Vassilev Moataz Salim Christina Popescu Cecilia Flores Giovanni Hernandez Low-cost conditioned place preference setup including video recording and analysis of behaviour MethodsX CPP animal tracking models of reinforcement Raspberry Pi |
| title | Low-cost conditioned place preference setup including video recording and analysis of behaviour |
| title_full | Low-cost conditioned place preference setup including video recording and analysis of behaviour |
| title_fullStr | Low-cost conditioned place preference setup including video recording and analysis of behaviour |
| title_full_unstemmed | Low-cost conditioned place preference setup including video recording and analysis of behaviour |
| title_short | Low-cost conditioned place preference setup including video recording and analysis of behaviour |
| title_sort | low cost conditioned place preference setup including video recording and analysis of behaviour |
| topic | CPP animal tracking models of reinforcement Raspberry Pi |
| url | http://www.sciencedirect.com/science/article/pii/S2215016120301187 |
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