Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.)
The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice (Oryza sativa L.), aberrant-floral spikelet 1 (afs1), which was derived from treatment of Xinong 1B wi...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-04-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919628479 |
| _version_ | 1819092813107691520 |
|---|---|
| author | Ting ZHANG Jing YOU Guo-ling YU Yi ZHANG Huan CHEN Yi-dan LI Li YE Wan-yue YAO Yu-jie TU Ying-hua LING Guang-hua HE Yun-feng LI |
| author_facet | Ting ZHANG Jing YOU Guo-ling YU Yi ZHANG Huan CHEN Yi-dan LI Li YE Wan-yue YAO Yu-jie TU Ying-hua LING Guang-hua HE Yun-feng LI |
| author_sort | Ting ZHANG |
| collection | DOAJ |
| description | The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice (Oryza sativa L.), aberrant-floral spikelet 1 (afs1), which was derived from treatment of Xinong 1B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion (InDel) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04g32510/LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets. |
| first_indexed | 2024-12-21T23:01:34Z |
| format | Article |
| id | doaj.art-5a7775eb5cc34c37b3b21ec1503e489d |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T23:01:34Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-5a7775eb5cc34c37b3b21ec1503e489d2022-12-21T18:47:17ZengElsevierJournal of Integrative Agriculture2095-31192020-04-01194921930Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.)Ting ZHANG0Jing YOU1Guo-ling YU2Yi ZHANG3Huan CHEN4Yi-dan LI5Li YE6Wan-yue YAO7Yu-jie TU8Ying-hua LING9Guang-hua HE10Yun-feng LI11Correspondence ZHANG Ting; Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaCorrespondence YOU Jing; Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaCorrespondence LI Yun-feng, Tel: +86-23-68250486; Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.ChinaThe spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice (Oryza sativa L.), aberrant-floral spikelet 1 (afs1), which was derived from treatment of Xinong 1B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion (InDel) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04g32510/LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets.http://www.sciencedirect.com/science/article/pii/S2095311919628479riceaberrant-floral spikelet 1spikeletgene mappingyield |
| spellingShingle | Ting ZHANG Jing YOU Guo-ling YU Yi ZHANG Huan CHEN Yi-dan LI Li YE Wan-yue YAO Yu-jie TU Ying-hua LING Guang-hua HE Yun-feng LI Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) Journal of Integrative Agriculture rice aberrant-floral spikelet 1 spikelet gene mapping yield |
| title | Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
| title_full | Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
| title_fullStr | Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
| title_full_unstemmed | Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
| title_short | Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.) |
| title_sort | gene mapping and candidate gene analysis of aberrant floral spikelet 1 afs1 in rice oryza sativa l |
| topic | rice aberrant-floral spikelet 1 spikelet gene mapping yield |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919628479 |
| work_keys_str_mv | AT tingzhang genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT jingyou genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT guolingyu genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT yizhang genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT huanchen genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT yidanli genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT liye genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT wanyueyao genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT yujietu genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT yinghualing genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT guanghuahe genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival AT yunfengli genemappingandcandidategeneanalysisofaberrantfloralspikelet1afs1inriceoryzasatival |