Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru
Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2009-04-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/15/4/08-1428_article |
| _version_ | 1818307218985975808 |
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| author | M. Steven Oberste Eduardo Gotuzzo Patrick Blair W. Allan Nix Thomas G. Ksiazek James A. Comer Pierre E. Rollin Cynthia S. Goldsmith James Olson Tadeusz J. Kochel |
| author_facet | M. Steven Oberste Eduardo Gotuzzo Patrick Blair W. Allan Nix Thomas G. Ksiazek James A. Comer Pierre E. Rollin Cynthia S. Goldsmith James Olson Tadeusz J. Kochel |
| author_sort | M. Steven Oberste |
| collection | DOAJ |
| description | Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription–PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness. |
| first_indexed | 2024-12-13T06:54:54Z |
| format | Article |
| id | doaj.art-5a790a4cb97940e996b385024b24b8d4 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T06:54:54Z |
| publishDate | 2009-04-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-5a790a4cb97940e996b385024b24b8d42022-12-21T23:56:04ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592009-04-0115464064610.3201/eid1504.081428Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, PeruM. Steven ObersteEduardo GotuzzoPatrick BlairW. Allan NixThomas G. KsiazekJames A. ComerPierre E. RollinCynthia S. GoldsmithJames OlsonTadeusz J. KochelEtiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients’ clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription–PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness.https://wwwnc.cdc.gov/eid/article/15/4/08-1428_articlePicornaviruscardiovirusencephalomyocarditis virusfebrile illnesshuman infectionsPeru |
| spellingShingle | M. Steven Oberste Eduardo Gotuzzo Patrick Blair W. Allan Nix Thomas G. Ksiazek James A. Comer Pierre E. Rollin Cynthia S. Goldsmith James Olson Tadeusz J. Kochel Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru Emerging Infectious Diseases Picornavirus cardiovirus encephalomyocarditis virus febrile illness human infections Peru |
| title | Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru |
| title_full | Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru |
| title_fullStr | Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru |
| title_full_unstemmed | Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru |
| title_short | Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru |
| title_sort | human febrile illness caused by encephalomyocarditis virus infection peru |
| topic | Picornavirus cardiovirus encephalomyocarditis virus febrile illness human infections Peru |
| url | https://wwwnc.cdc.gov/eid/article/15/4/08-1428_article |
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