Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data
The study aimed to perform the molecular identification of <i>Anisakis</i> larvae in commercial fish from the coast of the Canary Islands and to provide data on their infection level for the host and the species of this nematode parasite that we could find in several species of commercia...
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MDPI AG
2022-09-01
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| Loạt: | Animals |
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| Truy cập trực tuyến: | https://www.mdpi.com/2076-2615/12/19/2634 |
| _version_ | 1827655486398267392 |
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| author | Natalia Martin-Carrillo Katherine García-Livia Edgar Baz-González Néstor Abreu-Acosta Roberto Dorta-Guerra Basilio Valladares Pilar Foronda |
| author_facet | Natalia Martin-Carrillo Katherine García-Livia Edgar Baz-González Néstor Abreu-Acosta Roberto Dorta-Guerra Basilio Valladares Pilar Foronda |
| author_sort | Natalia Martin-Carrillo |
| collection | DOAJ |
| description | The study aimed to perform the molecular identification of <i>Anisakis</i> larvae in commercial fish from the coast of the Canary Islands and to provide data on their infection level for the host and the species of this nematode parasite that we could find in several species of commercial interest in the Canary Archipelago. Fish specimens (n = 172) from the Canary coasts were examined for parasites. In total, 495 larvae were identified; PCR was carried out for the entire ITS rDNA and cox2 mtDNA region, obtaining sixteen sequences for the entire ITS rDNA region and fifteen for the cox2 mtDNA, this being the first contribution of nucleotide sequences of <i>Anisakis</i> species of fish caught from the Canary Islands. An overall prevalence of 25% was obtained in the fish analyzed, and five species of <i>Anisakis</i> were identified, these being <i>Anisakis simplex</i> (s.s.), <i>Anisakis pegreffii, Anisakis physeteris</i>, <i>Anisakis nascettii</i> and <i>Anisakis typica</i> and the hybrid <i>Anisakis simplex</i> x <i>Anisakis pegreffii</i>. The results obtained in this study have relevance for public health, since the pathology will depend on the species of <i>Anisakis,</i> so it is important to know the health status of fish in the waters of the Canary Islands to assure a safer consumption and take adequate measures, in addition to the provision of epidemiological data. |
| first_indexed | 2024-03-09T22:08:24Z |
| format | Article |
| id | doaj.art-5a79e66fc8b54f63bcecfdac6fe6cde6 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:08:24Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5a79e66fc8b54f63bcecfdac6fe6cde62023-11-23T19:37:06ZengMDPI AGAnimals2076-26152022-09-011219263410.3390/ani12192634Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological DataNatalia Martin-Carrillo0Katherine García-Livia1Edgar Baz-González2Néstor Abreu-Acosta3Roberto Dorta-Guerra4Basilio Valladares5Pilar Foronda6Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, SpainThe study aimed to perform the molecular identification of <i>Anisakis</i> larvae in commercial fish from the coast of the Canary Islands and to provide data on their infection level for the host and the species of this nematode parasite that we could find in several species of commercial interest in the Canary Archipelago. Fish specimens (n = 172) from the Canary coasts were examined for parasites. In total, 495 larvae were identified; PCR was carried out for the entire ITS rDNA and cox2 mtDNA region, obtaining sixteen sequences for the entire ITS rDNA region and fifteen for the cox2 mtDNA, this being the first contribution of nucleotide sequences of <i>Anisakis</i> species of fish caught from the Canary Islands. An overall prevalence of 25% was obtained in the fish analyzed, and five species of <i>Anisakis</i> were identified, these being <i>Anisakis simplex</i> (s.s.), <i>Anisakis pegreffii, Anisakis physeteris</i>, <i>Anisakis nascettii</i> and <i>Anisakis typica</i> and the hybrid <i>Anisakis simplex</i> x <i>Anisakis pegreffii</i>. The results obtained in this study have relevance for public health, since the pathology will depend on the species of <i>Anisakis,</i> so it is important to know the health status of fish in the waters of the Canary Islands to assure a safer consumption and take adequate measures, in addition to the provision of epidemiological data.https://www.mdpi.com/2076-2615/12/19/2634<i>Anisakis simplex</i> (s.s.)<i>Anisakis pegreffii</i>Anisakis typica<i>Anisakis physeteris</i><i>Anisakis nascettii</i>Canary Islands |
| spellingShingle | Natalia Martin-Carrillo Katherine García-Livia Edgar Baz-González Néstor Abreu-Acosta Roberto Dorta-Guerra Basilio Valladares Pilar Foronda Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data Animals <i>Anisakis simplex</i> (s.s.) <i>Anisakis pegreffii</i> Anisakis typica <i>Anisakis physeteris</i> <i>Anisakis nascettii</i> Canary Islands |
| title | Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data |
| title_full | Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data |
| title_fullStr | Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data |
| title_full_unstemmed | Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data |
| title_short | Morphological and Molecular Identification of <i>Anisakis</i> spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data |
| title_sort | morphological and molecular identification of i anisakis i spp nematoda anisakidae in commercial fish from the canary islands coast spain epidemiological data |
| topic | <i>Anisakis simplex</i> (s.s.) <i>Anisakis pegreffii</i> Anisakis typica <i>Anisakis physeteris</i> <i>Anisakis nascettii</i> Canary Islands |
| url | https://www.mdpi.com/2076-2615/12/19/2634 |
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