Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays
<i>Vaccinium myrtillus</i> fruit (bilberry) is well known for its high richness in anthocyanins, which may be responsible for its preventive effects on several oxidative and carbonyl stress-related pathologies. However, limited data are available regarding the antioxidant and antiglycati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/6/512 |
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| author | Didier Fraisse Alexis Bred Catherine Felgines François Senejoux |
| author_facet | Didier Fraisse Alexis Bred Catherine Felgines François Senejoux |
| author_sort | Didier Fraisse |
| collection | DOAJ |
| description | <i>Vaccinium myrtillus</i> fruit (bilberry) is well known for its high richness in anthocyanins, which may be responsible for its preventive effects on several oxidative and carbonyl stress-related pathologies. However, limited data are available regarding the antioxidant and antiglycative contributions of its constituents. Spectrometric analyses were performed to evaluate anthocyanin content, radical scavenging and antiglycative properties of an anthocyanin-rich extract from bilberries. Additionally, original DPPH and methylglyoxal pre-column HPLC methods were instigated to allow straightforward identification of the main contributors to radical and carbonyl trapping effects. Finally, representative pure anthocyanins were evaluated using classical DPPH and antiglycation assays. Delphinidin, petunidin and cyanidin glycosides were identified as the most effective radical scavenging constituents in both HPLC and spectrometric DPPH evaluations. Potent antiglycative activities were also assessed for cyanidin, delphinidin and petunidin glucosides as attested by their respective IC<sub>50</sub> values of 114.2 ± 7.8, 130.5 ± 2.8, and 132.4 ± 3.7 µM. Interestingly, methylglyoxal spiking evaluation demonstrated that all bilberry anthocyanins exerted noticeable and comparable α-dicarbonyl trapping effects. Anthocyanins can be regarded as potent antiglycoxidant agents that might account for some health benefits of bilberries consumption. Besides, significant differences in their contributions were successfully highlighted by the employed pre-column HPLC assays. |
| first_indexed | 2024-03-10T19:14:57Z |
| format | Article |
| id | doaj.art-5a7a2d016d1f46dc8a8f1eada2e993d2 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T19:14:57Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5a7a2d016d1f46dc8a8f1eada2e993d22023-11-20T03:29:01ZengMDPI AGAntioxidants2076-39212020-06-019651210.3390/antiox9060512Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC AssaysDidier Fraisse0Alexis Bred1Catherine Felgines2François Senejoux3Human Nutition Unit and ECREIN Team, Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, FranceHuman Nutition Unit and ECREIN Team, Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, FranceHuman Nutition Unit and ECREIN Team, Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, FranceHuman Nutition Unit and ECREIN Team, Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, France<i>Vaccinium myrtillus</i> fruit (bilberry) is well known for its high richness in anthocyanins, which may be responsible for its preventive effects on several oxidative and carbonyl stress-related pathologies. However, limited data are available regarding the antioxidant and antiglycative contributions of its constituents. Spectrometric analyses were performed to evaluate anthocyanin content, radical scavenging and antiglycative properties of an anthocyanin-rich extract from bilberries. Additionally, original DPPH and methylglyoxal pre-column HPLC methods were instigated to allow straightforward identification of the main contributors to radical and carbonyl trapping effects. Finally, representative pure anthocyanins were evaluated using classical DPPH and antiglycation assays. Delphinidin, petunidin and cyanidin glycosides were identified as the most effective radical scavenging constituents in both HPLC and spectrometric DPPH evaluations. Potent antiglycative activities were also assessed for cyanidin, delphinidin and petunidin glucosides as attested by their respective IC<sub>50</sub> values of 114.2 ± 7.8, 130.5 ± 2.8, and 132.4 ± 3.7 µM. Interestingly, methylglyoxal spiking evaluation demonstrated that all bilberry anthocyanins exerted noticeable and comparable α-dicarbonyl trapping effects. Anthocyanins can be regarded as potent antiglycoxidant agents that might account for some health benefits of bilberries consumption. Besides, significant differences in their contributions were successfully highlighted by the employed pre-column HPLC assays.https://www.mdpi.com/2076-3921/9/6/512anthocyaninsdelphinidinpetunidincyanidin glycosidesbilberrypre-column HPLC method |
| spellingShingle | Didier Fraisse Alexis Bred Catherine Felgines François Senejoux Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays Antioxidants anthocyanins delphinidin petunidin cyanidin glycosides bilberry pre-column HPLC method |
| title | Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays |
| title_full | Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays |
| title_fullStr | Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays |
| title_full_unstemmed | Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays |
| title_short | Screening and Characterization of Antiglycoxidant Anthocyanins from <i>Vaccinium myrtillus</i> Fruit Using DPPH and Methylglyoxal Pre-Column HPLC Assays |
| title_sort | screening and characterization of antiglycoxidant anthocyanins from i vaccinium myrtillus i fruit using dpph and methylglyoxal pre column hplc assays |
| topic | anthocyanins delphinidin petunidin cyanidin glycosides bilberry pre-column HPLC method |
| url | https://www.mdpi.com/2076-3921/9/6/512 |
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