CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer
To investigate the role of TWIST1 in tumor angiogenesis in epithelial ovarian cancer and to identify key molecules involved in angiogenesis. TWIST1 small interfering RNA was transfected into A2780 cells, while a complementary DNA vector was transfected into non-malignant human ovarian surface epithe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOS Press
2017-05-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317706226 |
| _version_ | 1818725266934988800 |
|---|---|
| author | Yu-Jin Koo Tae-Jin Kim Kyung-Jin Min Kyeong-A So Un-Suk Jung Jin-Hwa Hong |
| author_facet | Yu-Jin Koo Tae-Jin Kim Kyung-Jin Min Kyeong-A So Un-Suk Jung Jin-Hwa Hong |
| author_sort | Yu-Jin Koo |
| collection | DOAJ |
| description | To investigate the role of TWIST1 in tumor angiogenesis in epithelial ovarian cancer and to identify key molecules involved in angiogenesis. TWIST1 small interfering RNA was transfected into A2780 cells, while a complementary DNA vector was transfected into non-malignant human ovarian surface epithelial cells to generate a TWIST1-overexpressing cell line. To evaluate how this affects angiogenesis, human umbilical vein endothelial cell tube formation assays were performed using the control and transfected cell lines. An antibody-based cytokine array was used to identify the molecules involved in TWIST1-mediated angiogenesis. After knockdown of TWIST1 via transfection of TWIST1 small interfering RNA into A2780 cells, the number of tubes formed by human umbilical vein endothelial cells significantly decreased in a tube formation assay. In a cytokine array, TWIST1 downregulation did not significantly decrease the secretion of the common pro-angiogenic factor, vascular endothelial growth factor, but instead inhibited the expression of the CXC chemokine ligand 11, which was confirmed by both an enzyme-linked immunosorbent assay and western blotting. In contrast, TWIST1 overexpression resulted in increased secretion of CXC chemokine ligand 11. Conversely, CXC chemokine ligand 11 downregulation did not inhibit the expression of TWIST1. Furthermore, the ability of TWIST1-expressing A2780 cells to induce angiogenesis was found to be inhibited after CXC chemokine ligand 11 knockdown in a tube formation assay. TWIST1 plays an important role in angiogenesis in epithelial ovarian cancer and is mediated by a novel pro-angiogenic factor, CXC chemokine ligand 11. Downregulation of CXC chemokine ligand 11 can inhibit tumor angiogenesis, suggesting that anti–CXC chemokine ligand 11 therapy may offer an alternative treatment strategy for TWIST1-positive ovarian cancer. |
| first_indexed | 2024-12-17T21:39:35Z |
| format | Article |
| id | doaj.art-5a7d19b555ca480b93ecee4cce41de2e |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-17T21:39:35Z |
| publishDate | 2017-05-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-5a7d19b555ca480b93ecee4cce41de2e2022-12-21T21:31:39ZengIOS PressTumor Biology1423-03802017-05-013910.1177/1010428317706226CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancerYu-Jin Koo0Tae-Jin Kim1Kyung-Jin Min2Kyeong-A So3Un-Suk Jung4Jin-Hwa Hong5Department of Obstetrics and Gynecology, Yeungnam University Medical Center, Daegu, KoreaDepartment of Obstetrics and Gynecology, Cheil General Hospital and Women’s Healthcare Center, Dankook University College of Medicine, Seoul, KoreaDepartment of Obstetrics and Gynecology, Korea University Ansan Hospital, Ansan, KoreaDepartment of Obstetrics and Gynecology, Cheil General Hospital and Women’s Healthcare Center, Dankook University College of Medicine, Seoul, KoreaDepartment of Obstetrics and Gynecology, Hanyang University Guri Hospital, Guri, KoreaDepartment of Obstetrics and Gynecology, Korea University Guro Hospital, Seoul, KoreaTo investigate the role of TWIST1 in tumor angiogenesis in epithelial ovarian cancer and to identify key molecules involved in angiogenesis. TWIST1 small interfering RNA was transfected into A2780 cells, while a complementary DNA vector was transfected into non-malignant human ovarian surface epithelial cells to generate a TWIST1-overexpressing cell line. To evaluate how this affects angiogenesis, human umbilical vein endothelial cell tube formation assays were performed using the control and transfected cell lines. An antibody-based cytokine array was used to identify the molecules involved in TWIST1-mediated angiogenesis. After knockdown of TWIST1 via transfection of TWIST1 small interfering RNA into A2780 cells, the number of tubes formed by human umbilical vein endothelial cells significantly decreased in a tube formation assay. In a cytokine array, TWIST1 downregulation did not significantly decrease the secretion of the common pro-angiogenic factor, vascular endothelial growth factor, but instead inhibited the expression of the CXC chemokine ligand 11, which was confirmed by both an enzyme-linked immunosorbent assay and western blotting. In contrast, TWIST1 overexpression resulted in increased secretion of CXC chemokine ligand 11. Conversely, CXC chemokine ligand 11 downregulation did not inhibit the expression of TWIST1. Furthermore, the ability of TWIST1-expressing A2780 cells to induce angiogenesis was found to be inhibited after CXC chemokine ligand 11 knockdown in a tube formation assay. TWIST1 plays an important role in angiogenesis in epithelial ovarian cancer and is mediated by a novel pro-angiogenic factor, CXC chemokine ligand 11. Downregulation of CXC chemokine ligand 11 can inhibit tumor angiogenesis, suggesting that anti–CXC chemokine ligand 11 therapy may offer an alternative treatment strategy for TWIST1-positive ovarian cancer.https://doi.org/10.1177/1010428317706226 |
| spellingShingle | Yu-Jin Koo Tae-Jin Kim Kyung-Jin Min Kyeong-A So Un-Suk Jung Jin-Hwa Hong CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer Tumor Biology |
| title | CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer |
| title_full | CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer |
| title_fullStr | CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer |
| title_full_unstemmed | CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer |
| title_short | CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer |
| title_sort | cxcl11 mediates twist1 induced angiogenesis in epithelial ovarian cancer |
| url | https://doi.org/10.1177/1010428317706226 |
| work_keys_str_mv | AT yujinkoo cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer AT taejinkim cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer AT kyungjinmin cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer AT kyeongaso cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer AT unsukjung cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer AT jinhwahong cxcl11mediatestwist1inducedangiogenesisinepithelialovariancancer |