A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

<p>Abstract</p> <p>Background</p> <p>The high-throughput anchoring of genetic markers into contigs is required for many ongoing physical mapping projects. Multidimentional BAC pooling strategies for PCR-based screening of large insert libraries is a widely used alternat...

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Bibliographic Details
Main Authors: Caligari Peter DS, Vu Giang TH, Wilkinson Mike J
Format: Article
Language:English
Published: BMC 2010-05-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/11/301

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