ExCyto PCR amplification.
ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start PCR amplification of cloned sequences without...
Main Authors: | Vinay Dhodda, Ronald Godiska, Jeffrey D Vanwye, David Mead, Rebecca Hochstein, Lynne Sheets, Sarah Vande Zande, Chris Niebauer, Douglas L Crawford, Marjorie F Oleksiak |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-09-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2935887?pdf=render |
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