Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra
We aimed to investigate the occurrence, phylogeny, and virulence of <i>E. coli</i> in the uterine contents and urine of female dogs with pyometra, through the presence of virulence genes and their genetic similarity. Uterine secretions and urine samples from 52 female dogs with pyometra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/4/158 |
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| author | Roberta T. Melo Raquel P. Oliveira Beatryz F. Silva Guilherme P. Monteiro João Paulo E. Saut Letícia R. M. Costa Sthéfany Da C. Dias Daise A. Rossi |
| author_facet | Roberta T. Melo Raquel P. Oliveira Beatryz F. Silva Guilherme P. Monteiro João Paulo E. Saut Letícia R. M. Costa Sthéfany Da C. Dias Daise A. Rossi |
| author_sort | Roberta T. Melo |
| collection | DOAJ |
| description | We aimed to investigate the occurrence, phylogeny, and virulence of <i>E. coli</i> in the uterine contents and urine of female dogs with pyometra, through the presence of virulence genes and their genetic similarity. Uterine secretions and urine samples from 52 female dogs with pyometra were collected and cultured. Strains identified as <i>E. coli</i> from 25 uterine and 7 urine samples were tested for virulence genes by PCR. Genetic similarity between the isolates was studied using RAPD-PCR. <i>E. coli</i> was observed in 48.07% uterine samples with pyometra and 20.0% urine samples. The strains showed high percentages for the presence of virulence genes: 96.9% had the gene <i>sfa</i>, 59.4% <i>afa,</i> 46.9% <i>pap</i>, 53.1% <i>hly</i>, and 68.75% <i>cnf</i>. Even with the high prevalence of virulence genes, the samples were not submitted to DNA sequencing to confirm the results. Analysis showed high genetic diversity in <i>E. coli,</i> however, strains isolated from the same animal indicate that cystitis and pyometra could be related. Our study indicated the association between <i>E. coli</i> in dogs with pyometra and cases of urinary tract infection and the pathogenic potential of strains increasing with animal age. |
| first_indexed | 2024-03-09T10:28:06Z |
| format | Article |
| id | doaj.art-5a82515233b74c4cb38a8034697d1de2 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T10:28:06Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-5a82515233b74c4cb38a8034697d1de22023-12-01T21:31:26ZengMDPI AGVeterinary Sciences2306-73812022-03-019415810.3390/vetsci9040158Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with PyometraRoberta T. Melo0Raquel P. Oliveira1Beatryz F. Silva2Guilherme P. Monteiro3João Paulo E. Saut4Letícia R. M. Costa5Sthéfany Da C. Dias6Daise A. Rossi7Laboratório de Biotecnologia Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilHospital Veterinário, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilHospital Veterinário, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilLaboratório de Biotecnologia Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilLaboratório de Saúde em Grandes Animais, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilLaboratório de Biotecnologia Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilLaboratório de Biotecnologia Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilLaboratório de Biotecnologia Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia 38402-018, MG, BrazilWe aimed to investigate the occurrence, phylogeny, and virulence of <i>E. coli</i> in the uterine contents and urine of female dogs with pyometra, through the presence of virulence genes and their genetic similarity. Uterine secretions and urine samples from 52 female dogs with pyometra were collected and cultured. Strains identified as <i>E. coli</i> from 25 uterine and 7 urine samples were tested for virulence genes by PCR. Genetic similarity between the isolates was studied using RAPD-PCR. <i>E. coli</i> was observed in 48.07% uterine samples with pyometra and 20.0% urine samples. The strains showed high percentages for the presence of virulence genes: 96.9% had the gene <i>sfa</i>, 59.4% <i>afa,</i> 46.9% <i>pap</i>, 53.1% <i>hly</i>, and 68.75% <i>cnf</i>. Even with the high prevalence of virulence genes, the samples were not submitted to DNA sequencing to confirm the results. Analysis showed high genetic diversity in <i>E. coli,</i> however, strains isolated from the same animal indicate that cystitis and pyometra could be related. Our study indicated the association between <i>E. coli</i> in dogs with pyometra and cases of urinary tract infection and the pathogenic potential of strains increasing with animal age.https://www.mdpi.com/2306-7381/9/4/158<i>E. coli</i>canine pyometraurineuterusvirulence genes |
| spellingShingle | Roberta T. Melo Raquel P. Oliveira Beatryz F. Silva Guilherme P. Monteiro João Paulo E. Saut Letícia R. M. Costa Sthéfany Da C. Dias Daise A. Rossi Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra Veterinary Sciences <i>E. coli</i> canine pyometra urine uterus virulence genes |
| title | Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra |
| title_full | Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra |
| title_fullStr | Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra |
| title_full_unstemmed | Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra |
| title_short | Phylogeny and Virulence Factors of <i>Escherichia coli</i> Isolated from Dogs with Pyometra |
| title_sort | phylogeny and virulence factors of i escherichia coli i isolated from dogs with pyometra |
| topic | <i>E. coli</i> canine pyometra urine uterus virulence genes |
| url | https://www.mdpi.com/2306-7381/9/4/158 |
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