Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology
Plant-based food intake is essential for health promotion, especially in preventing chronic diseases. Flavonoids, as important bioactive substances found in citrus fruits, have a variety of health benefits, including antioxidant, anti-inflammatory, and cancer-inhibiting properties. This study used u...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X24000558 |
| _version_ | 1797272413812031488 |
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| author | Rusheng Jin Yuanxing Wang |
| author_facet | Rusheng Jin Yuanxing Wang |
| author_sort | Rusheng Jin |
| collection | DOAJ |
| description | Plant-based food intake is essential for health promotion, especially in preventing chronic diseases. Flavonoids, as important bioactive substances found in citrus fruits, have a variety of health benefits, including antioxidant, anti-inflammatory, and cancer-inhibiting properties. This study used ultra-high performance liquid chromatography tandem quadrupole time of flight mass spectrometry to determine the flavonoids in the flavedo of navel oranges at different growth stages. Combined with chemometric methods to distinguish flavonoids at different growth stages, 15 differential flavonoids were deduced. In addition, the biomarker flavonoids in navel orange flavedo were quantified by high performance liquid chromatography mass spectrometry. The results showed that the contents of rutin, isosinensetin, and isorhamnetin all showed a trend of increasing and then decreasing during the growing period, while the contents of tangeretin and nobiletin would gradually decrease with the increase of fruit maturity. In addition, the analysis also revealed that the content of isorhamnetin was negatively correlated with the content of both narirutin and tangeretin, and it was hypothesized that isorhamnetin was in the same metabolic branching pathway as narirutin or tangeretin. The results of this study support the practical application of orange peel as a nutritional resource in functional foods. |
| first_indexed | 2024-03-07T14:27:58Z |
| format | Article |
| id | doaj.art-5a826196f4e641f2b14065ff9297f5b9 |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-03-07T14:27:58Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-5a826196f4e641f2b14065ff9297f5b92024-03-06T05:29:17ZengElsevierFood Chemistry Advances2772-753X2024-06-014100659Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technologyRusheng Jin0Yuanxing Wang1State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China; College of Food Science, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China; College of Food Science, Nanchang University, Nanchang 330047, China; Corresponding author at: State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi 330047, China.Plant-based food intake is essential for health promotion, especially in preventing chronic diseases. Flavonoids, as important bioactive substances found in citrus fruits, have a variety of health benefits, including antioxidant, anti-inflammatory, and cancer-inhibiting properties. This study used ultra-high performance liquid chromatography tandem quadrupole time of flight mass spectrometry to determine the flavonoids in the flavedo of navel oranges at different growth stages. Combined with chemometric methods to distinguish flavonoids at different growth stages, 15 differential flavonoids were deduced. In addition, the biomarker flavonoids in navel orange flavedo were quantified by high performance liquid chromatography mass spectrometry. The results showed that the contents of rutin, isosinensetin, and isorhamnetin all showed a trend of increasing and then decreasing during the growing period, while the contents of tangeretin and nobiletin would gradually decrease with the increase of fruit maturity. In addition, the analysis also revealed that the content of isorhamnetin was negatively correlated with the content of both narirutin and tangeretin, and it was hypothesized that isorhamnetin was in the same metabolic branching pathway as narirutin or tangeretin. The results of this study support the practical application of orange peel as a nutritional resource in functional foods.http://www.sciencedirect.com/science/article/pii/S2772753X24000558FlavonoidsFlavedoUPLC-QTOF-MSHPLC-QQQ-MSChemometricsMetabolic pathway |
| spellingShingle | Rusheng Jin Yuanxing Wang Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology Food Chemistry Advances Flavonoids Flavedo UPLC-QTOF-MS HPLC-QQQ-MS Chemometrics Metabolic pathway |
| title | Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| title_full | Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| title_fullStr | Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| title_full_unstemmed | Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| title_short | Targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| title_sort | targeted screening and quantification of flavonoids in the flavedo of navel oranges at different growth stages based on metabolomics technology |
| topic | Flavonoids Flavedo UPLC-QTOF-MS HPLC-QQQ-MS Chemometrics Metabolic pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X24000558 |
| work_keys_str_mv | AT rushengjin targetedscreeningandquantificationofflavonoidsintheflavedoofnavelorangesatdifferentgrowthstagesbasedonmetabolomicstechnology AT yuanxingwang targetedscreeningandquantificationofflavonoidsintheflavedoofnavelorangesatdifferentgrowthstagesbasedonmetabolomicstechnology |