Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature
Temperature is one of the major factors that affect the outbreak of infectious disease. Lines of evidences have shown that virulence factors can be controlled by thermo-sensors in bacterial pathogens. However, how temperature influences host's responses to the pathogen is still largely unexplor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01991/full |
| _version_ | 1811222474364289024 |
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| author | Ming Jiang Ming Jiang Zhuang-gui Chen Jun Zheng Bo Peng Bo Peng Bo Peng Bo Peng |
| author_facet | Ming Jiang Ming Jiang Zhuang-gui Chen Jun Zheng Bo Peng Bo Peng Bo Peng Bo Peng |
| author_sort | Ming Jiang |
| collection | DOAJ |
| description | Temperature is one of the major factors that affect the outbreak of infectious disease. Lines of evidences have shown that virulence factors can be controlled by thermo-sensors in bacterial pathogens. However, how temperature influences host's responses to the pathogen is still largely unexplored, and the study of this might pave the way to develop strategies to manage pathogenic bacterial infection. In the present study, we show that finfish Carassius carassius, the crucian carp that is tolerant to a wide range of temperatures, is less susceptible to bacterial infection when grown in 20°C than in 30°C. The different responses of C. carassius to bacterial infection could be partially explained by the distinct metabolisms under the specific temperatures: C. carassius shows elevated tricarboxylic acid cycle (TCA cycle) but decreased taurine and hypotaurine metabolism as well as lower biosynthesis of unsaturated fatty acids at 30°C. The decreased abundance of palmitate, threonine, and taurine represents the most characteristic metabolic feature. Consistently, exogenous palmitate, threonine, or taurine enhances the survival of C. carassius to bacterial infection at 30°C in a dose-dependent manner. This effect could be attributed to the inhibition on the TCA cycle by the three metabolites. This notion is further supported by the fact that low concentration of malonate, a succinate dehydrogenase inhibitor, increases the survival of C. carassius at 30°C as well. On the other hand, addition of the three metabolites rescued the decreased expression of pro-inflammatory cytokines including TNF-α1, TNF-α2, IL-1β1, IL-1β2, and lysozyme at 30°C. Taken together, our results revealed an unexpected relationship between temperature and metabolism that orchestrates the immune regulation against infection by bacterial pathogens. Thus, this study shed light on the modulation of finfish physiology to fight against bacterial infection through metabolism. |
| first_indexed | 2024-04-12T08:16:32Z |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T08:16:32Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-5a8294d44534479a80b1e177bf8579b82022-12-22T03:40:46ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.01991468953Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water TemperatureMing Jiang0Ming Jiang1Zhuang-gui Chen2Jun Zheng3Bo Peng4Bo Peng5Bo Peng6Bo Peng7State Key Laboratory of Bio-Control, Higher Education Mega Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaFaculty of Health Sciences, University of Macau, Macau, ChinaState Key Laboratory of Bio-Control, Higher Education Mega Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaSouthern Marine Science and Engineering Guangdong Laboratory, Zhuhai, ChinaTemperature is one of the major factors that affect the outbreak of infectious disease. Lines of evidences have shown that virulence factors can be controlled by thermo-sensors in bacterial pathogens. However, how temperature influences host's responses to the pathogen is still largely unexplored, and the study of this might pave the way to develop strategies to manage pathogenic bacterial infection. In the present study, we show that finfish Carassius carassius, the crucian carp that is tolerant to a wide range of temperatures, is less susceptible to bacterial infection when grown in 20°C than in 30°C. The different responses of C. carassius to bacterial infection could be partially explained by the distinct metabolisms under the specific temperatures: C. carassius shows elevated tricarboxylic acid cycle (TCA cycle) but decreased taurine and hypotaurine metabolism as well as lower biosynthesis of unsaturated fatty acids at 30°C. The decreased abundance of palmitate, threonine, and taurine represents the most characteristic metabolic feature. Consistently, exogenous palmitate, threonine, or taurine enhances the survival of C. carassius to bacterial infection at 30°C in a dose-dependent manner. This effect could be attributed to the inhibition on the TCA cycle by the three metabolites. This notion is further supported by the fact that low concentration of malonate, a succinate dehydrogenase inhibitor, increases the survival of C. carassius at 30°C as well. On the other hand, addition of the three metabolites rescued the decreased expression of pro-inflammatory cytokines including TNF-α1, TNF-α2, IL-1β1, IL-1β2, and lysozyme at 30°C. Taken together, our results revealed an unexpected relationship between temperature and metabolism that orchestrates the immune regulation against infection by bacterial pathogens. Thus, this study shed light on the modulation of finfish physiology to fight against bacterial infection through metabolism.https://www.frontiersin.org/article/10.3389/fimmu.2019.01991/fullwater temperaturebacterial infectionCarassius carassiusmetabolomeinnate immunity |
| spellingShingle | Ming Jiang Ming Jiang Zhuang-gui Chen Jun Zheng Bo Peng Bo Peng Bo Peng Bo Peng Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature Frontiers in Immunology water temperature bacterial infection Carassius carassius metabolome innate immunity |
| title | Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature |
| title_full | Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature |
| title_fullStr | Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature |
| title_full_unstemmed | Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature |
| title_short | Metabolites-Enabled Survival of Crucian Carps Infected by Edwardsiella tarda in High Water Temperature |
| title_sort | metabolites enabled survival of crucian carps infected by edwardsiella tarda in high water temperature |
| topic | water temperature bacterial infection Carassius carassius metabolome innate immunity |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01991/full |
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