The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model
Sulfur mustard (SM)-induced ocular injury is characterized by an acute inflammatory response that may become chronic or enter a latent phase with delayed pathology. This study aimed to evaluate the efficacy of ziv-aflibercept and aflibercept in preventing and ameliorating corneal neovascularization...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750023000136 |
| _version_ | 1797798871935483904 |
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| author | Ariel Gore Tamar Kadar Maayan Cohen Hila Gutman Rellie Gez Shlomit Dachir Vered Horwitz |
| author_facet | Ariel Gore Tamar Kadar Maayan Cohen Hila Gutman Rellie Gez Shlomit Dachir Vered Horwitz |
| author_sort | Ariel Gore |
| collection | DOAJ |
| description | Sulfur mustard (SM)-induced ocular injury is characterized by an acute inflammatory response that may become chronic or enter a latent phase with delayed pathology. This study aimed to evaluate the efficacy of ziv-aflibercept and aflibercept in preventing and ameliorating corneal neovascularization (NV), respectively, following chemical eye exposure to SM vapor in a rabbit model. Chemical SM ocular insult was induced in the right eye of rabbits. A single application of ziv-aflibercept was administered 2 h or 9 days post-exposure. A single subconjunctival aflibercept treatment in an ocular formulation was administered 4 weeks after SM vapor exposure and subsequent to an initial 1-week treatment with 0.1 % dexamethasone. Clinical monitoring was performed 5–12 weeks post-exposure, and digital corneal pictures were taken to assess the extent of NV. The rabbits were euthanized and the corneas were processed for histological assessment. Treatment with ziv-aflibercept 2 h and 9 days post-exposure moderately reduced insult severity and partially delayed or prevented corneal NV. Aflibercept application 4 weeks post-exposure significantly reduced the extent of NV for 8 weeks. The substantial decrease in existing corneal NV in this group was confirmed by histology. These results reveal the powerful anti-angiogenic efficacy of the VEGF-trap for ameliorating existing NV as opposed to preventing NV development, revealing the ability of this treatment to mitigate corneal NV. |
| first_indexed | 2024-03-13T04:10:58Z |
| format | Article |
| id | doaj.art-5a86abe24db54c258249ac45e0d26573 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-03-13T04:10:58Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-5a86abe24db54c258249ac45e0d265732023-06-21T06:55:03ZengElsevierToxicology Reports2214-75002023-01-0110206215The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit modelAriel Gore0Tamar Kadar1Maayan Cohen2Hila Gutman3Rellie Gez4Shlomit Dachir5Vered Horwitz6Corresponding author.; Department. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelDepartment. of Pharmacology, Israel Institute for Biological Research, Ness Ziona 74100, IsraelSulfur mustard (SM)-induced ocular injury is characterized by an acute inflammatory response that may become chronic or enter a latent phase with delayed pathology. This study aimed to evaluate the efficacy of ziv-aflibercept and aflibercept in preventing and ameliorating corneal neovascularization (NV), respectively, following chemical eye exposure to SM vapor in a rabbit model. Chemical SM ocular insult was induced in the right eye of rabbits. A single application of ziv-aflibercept was administered 2 h or 9 days post-exposure. A single subconjunctival aflibercept treatment in an ocular formulation was administered 4 weeks after SM vapor exposure and subsequent to an initial 1-week treatment with 0.1 % dexamethasone. Clinical monitoring was performed 5–12 weeks post-exposure, and digital corneal pictures were taken to assess the extent of NV. The rabbits were euthanized and the corneas were processed for histological assessment. Treatment with ziv-aflibercept 2 h and 9 days post-exposure moderately reduced insult severity and partially delayed or prevented corneal NV. Aflibercept application 4 weeks post-exposure significantly reduced the extent of NV for 8 weeks. The substantial decrease in existing corneal NV in this group was confirmed by histology. These results reveal the powerful anti-angiogenic efficacy of the VEGF-trap for ameliorating existing NV as opposed to preventing NV development, revealing the ability of this treatment to mitigate corneal NV.http://www.sciencedirect.com/science/article/pii/S2214750023000136Ocular chemical insultOcular surfaceSulfur mustardNeovascularizationAflibercept |
| spellingShingle | Ariel Gore Tamar Kadar Maayan Cohen Hila Gutman Rellie Gez Shlomit Dachir Vered Horwitz The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model Toxicology Reports Ocular chemical insult Ocular surface Sulfur mustard Neovascularization Aflibercept |
| title | The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model |
| title_full | The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model |
| title_fullStr | The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model |
| title_full_unstemmed | The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model |
| title_short | The use of aflibercept (VEGF trap) in mitigating sulfur mustard-induced corneal neovascularization in a rabbit model |
| title_sort | use of aflibercept vegf trap in mitigating sulfur mustard induced corneal neovascularization in a rabbit model |
| topic | Ocular chemical insult Ocular surface Sulfur mustard Neovascularization Aflibercept |
| url | http://www.sciencedirect.com/science/article/pii/S2214750023000136 |
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