Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis
Abstract Background This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis (EPTB). Methods From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-020-05036-0 |
| _version_ | 1818233043172720640 |
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| author | Tongxin Li Tao Shi Ying Sun Kan Zhou Zhenggu Huang Pengsen Wang Ming Luo Xiaoping Nie Guoqiang Yang Yu Chen Yaokai Chen |
| author_facet | Tongxin Li Tao Shi Ying Sun Kan Zhou Zhenggu Huang Pengsen Wang Ming Luo Xiaoping Nie Guoqiang Yang Yu Chen Yaokai Chen |
| author_sort | Tongxin Li |
| collection | DOAJ |
| description | Abstract Background This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis (EPTB). Methods From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including EPTB (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing (SAT) method. The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of EPTB. Results For 433 EPTB specimens and 49 non-TB specimens, the simultaneous amplification and testing tuberculosis (SAT-TB) results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6, 79.4, 59.4, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6 and 100%, respectively, the cultures test were 29.3 and 98.0%. Conclusions SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories. |
| first_indexed | 2024-12-12T11:15:54Z |
| format | Article |
| id | doaj.art-5a88a543f49948d7931d098c5dc87643 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T11:15:54Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-5a88a543f49948d7931d098c5dc876432022-12-22T00:26:10ZengBMCBMC Infectious Diseases1471-23342020-04-012011610.1186/s12879-020-05036-0Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosisTongxin Li0Tao Shi1Ying Sun2Kan Zhou3Zhenggu Huang4Pengsen Wang5Ming Luo6Xiaoping Nie7Guoqiang Yang8Yu Chen9Yaokai Chen10Central Laboratory, Chongqing Public Health Medical CenterDepartment of Orthopedics, Tianjin First Center HospitalDepartment of Respiratory, Tianjin Hexi HospitalCentral Laboratory, Chongqing Public Health Medical CenterCentral Laboratory, Chongqing Public Health Medical CenterCentral Laboratory, Chongqing Public Health Medical CenterCentral Laboratory, Chongqing Public Health Medical CenterDepartment of Clinical Laboratory, Chongqing Public Health Medical CenterDepartment of Orthopedics, Chongqing Public Health Medical CenterMedical Records Room, Chongqing Public Health Medical CenterCentral Laboratory, Chongqing Public Health Medical CenterAbstract Background This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis (EPTB). Methods From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including EPTB (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing (SAT) method. The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of EPTB. Results For 433 EPTB specimens and 49 non-TB specimens, the simultaneous amplification and testing tuberculosis (SAT-TB) results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6, 79.4, 59.4, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6 and 100%, respectively, the cultures test were 29.3 and 98.0%. Conclusions SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.http://link.springer.com/article/10.1186/s12879-020-05036-0Extra-pulmonaryMycobacterium tuberculosisSimultaneous amplification and testingSpecificitySensitivity |
| spellingShingle | Tongxin Li Tao Shi Ying Sun Kan Zhou Zhenggu Huang Pengsen Wang Ming Luo Xiaoping Nie Guoqiang Yang Yu Chen Yaokai Chen Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis BMC Infectious Diseases Extra-pulmonary Mycobacterium tuberculosis Simultaneous amplification and testing Specificity Sensitivity |
| title | Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis |
| title_full | Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis |
| title_fullStr | Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis |
| title_full_unstemmed | Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis |
| title_short | Application of real-time simultaneous amplification and testing method to accurately and rapidly detect extra-pulmonary tuberculosis |
| title_sort | application of real time simultaneous amplification and testing method to accurately and rapidly detect extra pulmonary tuberculosis |
| topic | Extra-pulmonary Mycobacterium tuberculosis Simultaneous amplification and testing Specificity Sensitivity |
| url | http://link.springer.com/article/10.1186/s12879-020-05036-0 |
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