The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway
ABSTRACT: To determine whether the antipyretic effect of the mixture of Radix isatidis, Forsythiae, and Gypsum (RIFG) on lipopolysaccharide (LPS) induced fever broilers and its related mechanisms. A total of 315 24-day-old yellow-plumed broilers were randomly divided into 7 groups, except for the co...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123005515 |
| _version_ | 1797660107451924480 |
|---|---|
| author | Huiting Wang Xiaoman Zheng Yongshi Lin Xirui Zheng Mingen Yan Yaoxing Li Dayou Shi Shining Guo Cui Liu |
| author_facet | Huiting Wang Xiaoman Zheng Yongshi Lin Xirui Zheng Mingen Yan Yaoxing Li Dayou Shi Shining Guo Cui Liu |
| author_sort | Huiting Wang |
| collection | DOAJ |
| description | ABSTRACT: To determine whether the antipyretic effect of the mixture of Radix isatidis, Forsythiae, and Gypsum (RIFG) on lipopolysaccharide (LPS) induced fever broilers and its related mechanisms. A total of 315 24-day-old yellow-plumed broilers were randomly divided into 7 groups, except for the control group, other groups were injected with LPS. Two hours later, RIFG were given drinking water to relieve fever, and it was evaluated by the expression of genes and proteins of the maximum body temperature rise (∆T), body temperature response index (TRI), serum and hypothalamic pyrogenic heat factor. RIFG could reduce the body temperature of broilers with fever (P < 0.01). It inhibited the expressions of IL-6 and PGE2 (P < 0.01), down-regulated mRNA expression levels of TNF-ɑ and COX-2 (P < 0.01), and promoted the generation of antipyretic factor AVP mRNA (P < 0.01). In addition, the expression level of TLR4 and NF-κB p65 protein can be down-regulated, and LPS + RM group has the best down-regulated effect. RIFG had a good antipyretic effect on reducing LPS-induced fever of broilers by inhibiting the activation of TLR4/NF-κB signaling pathway and thermogenic factors. |
| first_indexed | 2024-03-11T18:24:59Z |
| format | Article |
| id | doaj.art-5a890994781f447bbf01839f6afd589e |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T18:24:59Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-5a890994781f447bbf01839f6afd589e2023-10-14T04:43:53ZengElsevierPoultry Science0032-57912023-11-0110211103032The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathwayHuiting Wang0Xiaoman Zheng1Yongshi Lin2Xirui Zheng3Mingen Yan4Yaoxing Li5Dayou Shi6Shining Guo7Cui Liu8College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou, 510642, China; International Institute of Traditional Chinese Veterinary Medicine, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou, 510642, China; International Institute of Traditional Chinese Veterinary Medicine, Guangzhou, 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Corresponding author:ABSTRACT: To determine whether the antipyretic effect of the mixture of Radix isatidis, Forsythiae, and Gypsum (RIFG) on lipopolysaccharide (LPS) induced fever broilers and its related mechanisms. A total of 315 24-day-old yellow-plumed broilers were randomly divided into 7 groups, except for the control group, other groups were injected with LPS. Two hours later, RIFG were given drinking water to relieve fever, and it was evaluated by the expression of genes and proteins of the maximum body temperature rise (∆T), body temperature response index (TRI), serum and hypothalamic pyrogenic heat factor. RIFG could reduce the body temperature of broilers with fever (P < 0.01). It inhibited the expressions of IL-6 and PGE2 (P < 0.01), down-regulated mRNA expression levels of TNF-ɑ and COX-2 (P < 0.01), and promoted the generation of antipyretic factor AVP mRNA (P < 0.01). In addition, the expression level of TLR4 and NF-κB p65 protein can be down-regulated, and LPS + RM group has the best down-regulated effect. RIFG had a good antipyretic effect on reducing LPS-induced fever of broilers by inhibiting the activation of TLR4/NF-κB signaling pathway and thermogenic factors.http://www.sciencedirect.com/science/article/pii/S0032579123005515lipopolysaccharidebroilerTLR4/NF-κB signaling pathwayantipyretic effect |
| spellingShingle | Huiting Wang Xiaoman Zheng Yongshi Lin Xirui Zheng Mingen Yan Yaoxing Li Dayou Shi Shining Guo Cui Liu The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway Poultry Science lipopolysaccharide broiler TLR4/NF-κB signaling pathway antipyretic effect |
| title | The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway |
| title_full | The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway |
| title_fullStr | The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway |
| title_full_unstemmed | The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway |
| title_short | The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway |
| title_sort | mixture of radix isatidis forsythiae and gypsum alleviates lipopolysaccharide induced fever in broilers by inhibition of tlr4 nf κb signaling pathway |
| topic | lipopolysaccharide broiler TLR4/NF-κB signaling pathway antipyretic effect |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123005515 |
| work_keys_str_mv | AT huitingwang themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT xiaomanzheng themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT yongshilin themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT xiruizheng themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT mingenyan themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT yaoxingli themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT dayoushi themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT shiningguo themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT cuiliu themixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT huitingwang mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT xiaomanzheng mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT yongshilin mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT xiruizheng mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT mingenyan mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT yaoxingli mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT dayoushi mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT shiningguo mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway AT cuiliu mixtureofradixisatidisforsythiaeandgypsumalleviateslipopolysaccharideinducedfeverinbroilersbyinhibitionoftlr4nfkbsignalingpathway |