Disruption of microbial cell morphology by Buxus macowanii
Abstract Background Microbial infections are one of the major causes of death globally. This is attributed to the rising costs of primary healthcare and its inaccessibility especially in developing countries. Moreover, there has been an increase in microbial strains that have reduced susceptibility...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-020-03049-5 |
| _version_ | 1811323869156343808 |
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| author | B. Ngobeni S. S. Mashele N. J. Malebo E. van der Watt I. T. Manduna |
| author_facet | B. Ngobeni S. S. Mashele N. J. Malebo E. van der Watt I. T. Manduna |
| author_sort | B. Ngobeni |
| collection | DOAJ |
| description | Abstract Background Microbial infections are one of the major causes of death globally. This is attributed to the rising costs of primary healthcare and its inaccessibility especially in developing countries. Moreover, there has been an increase in microbial strains that have reduced susceptibility to antimicrobial drugs. Research on the antimicrobial properties of medicinal plants, which could address these problems, has become more important as they present fewer side effects when compared to the antibiotics currently in use. This study evaluated the antimicrobial properties of a methanolic extract from Buxus macowanii in order to assess its potential in the development of novel antimicrobial drugs. Methods Antimicrobial activity of the extract was evaluated using the broth microdilution method. The effects of B. macowanii on the morphology of B. cereus were observed using Scanning and Transmission electron microscopy. Chemical profiling of the plant extract was performed using the GCMS. Results The extract showed antimicrobial activity against all the microbial species used. Microscopic examination of the cells of B. cereus cells treated with Buxus macowanii showed some changes in morphology such as damage of the cell wall, swelling of the cells and incomplete cell division that eventually resulted in cell death. Neophytadiene, an antimicrobial compound was detected in the extract using GCMS. Conclusion The morphological disruptions of the cell wall of Bacillus cereus explain the antimicrobial properties of B. macowanii and indicate its possible application in the development of natural antimicrobial drugs. |
| first_indexed | 2024-04-13T14:04:16Z |
| format | Article |
| id | doaj.art-5a8d5582ec8f48c884f63b8cb4722932 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-13T14:04:16Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-5a8d5582ec8f48c884f63b8cb47229322022-12-22T02:43:58ZengBMCBMC Complementary Medicine and Therapies2662-76712020-08-012011810.1186/s12906-020-03049-5Disruption of microbial cell morphology by Buxus macowaniiB. Ngobeni0S. S. Mashele1N. J. Malebo2E. van der Watt3I. T. Manduna4Department of Health Sciences, Central University of TechnologyDepartment of Health Sciences, Central University of TechnologyDepartment of Life Sciences, Central University of TechnologyDepartment of Soil, Crop and Climate Sciences, University of the Free StateCentre for Applied Food Sustainability and Biotechnology (CAFSaB), Central University of TechnologyAbstract Background Microbial infections are one of the major causes of death globally. This is attributed to the rising costs of primary healthcare and its inaccessibility especially in developing countries. Moreover, there has been an increase in microbial strains that have reduced susceptibility to antimicrobial drugs. Research on the antimicrobial properties of medicinal plants, which could address these problems, has become more important as they present fewer side effects when compared to the antibiotics currently in use. This study evaluated the antimicrobial properties of a methanolic extract from Buxus macowanii in order to assess its potential in the development of novel antimicrobial drugs. Methods Antimicrobial activity of the extract was evaluated using the broth microdilution method. The effects of B. macowanii on the morphology of B. cereus were observed using Scanning and Transmission electron microscopy. Chemical profiling of the plant extract was performed using the GCMS. Results The extract showed antimicrobial activity against all the microbial species used. Microscopic examination of the cells of B. cereus cells treated with Buxus macowanii showed some changes in morphology such as damage of the cell wall, swelling of the cells and incomplete cell division that eventually resulted in cell death. Neophytadiene, an antimicrobial compound was detected in the extract using GCMS. Conclusion The morphological disruptions of the cell wall of Bacillus cereus explain the antimicrobial properties of B. macowanii and indicate its possible application in the development of natural antimicrobial drugs.http://link.springer.com/article/10.1186/s12906-020-03049-5Antimicrobial activityBuxus macowaniiNeophytadiene |
| spellingShingle | B. Ngobeni S. S. Mashele N. J. Malebo E. van der Watt I. T. Manduna Disruption of microbial cell morphology by Buxus macowanii BMC Complementary Medicine and Therapies Antimicrobial activity Buxus macowanii Neophytadiene |
| title | Disruption of microbial cell morphology by Buxus macowanii |
| title_full | Disruption of microbial cell morphology by Buxus macowanii |
| title_fullStr | Disruption of microbial cell morphology by Buxus macowanii |
| title_full_unstemmed | Disruption of microbial cell morphology by Buxus macowanii |
| title_short | Disruption of microbial cell morphology by Buxus macowanii |
| title_sort | disruption of microbial cell morphology by buxus macowanii |
| topic | Antimicrobial activity Buxus macowanii Neophytadiene |
| url | http://link.springer.com/article/10.1186/s12906-020-03049-5 |
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