Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines
Background and Aim: Breast cancer is the second leading cause of death among women worldwide. Despite recent advances in cancer treatment, this disease remains one of the leading causes of death. Snake venom is a mixture of various molecules such as carbohydrates, nucleosides, amino acids, lipids, p...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Kurdistan University of Medical Sciences
2022-01-01
|
| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
| Subjects: | |
| Online Access: | http://sjku.muk.ac.ir/article-1-5491-en.html |
| _version_ | 1818549434407976960 |
|---|---|
| author | Parsa Amirian Kaveh Shahveisi Mona Pazhouhi Cyrus Jalili |
| author_facet | Parsa Amirian Kaveh Shahveisi Mona Pazhouhi Cyrus Jalili |
| author_sort | Parsa Amirian |
| collection | DOAJ |
| description | Background and Aim: Breast cancer is the second leading cause of death among women worldwide. Despite recent advances in cancer treatment, this disease remains one of the leading causes of death. Snake venom is a mixture of various molecules such as carbohydrates, nucleosides, amino acids, lipids, proteins and peptides. Previous studies have shown that the venom of some snakes have anti-cancer effects on human cell lines. In the present study, the effect of Vipera raddei kurdistanica venom on breast cell lines was investigated.
Materials and Methods: The effect of increasing concentrations of snake venom on breast cells viability was investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and lactate dehydrogenase measurement. Apoptotic cell death was evaluated by fluorescent dye staining and DNA fragmentation assay. Changes in the expression levels of some apoptotic-related genes were investigated by using real time PCR. Data were analyzed by one-way ANOVA followed by Tukey's test. P <0.05 was considered significant.
Results: After 24, 48, 72 and 96 hours treatment, cell viability was significantly decreased in a time and dose dependent manner (P <0.05). The effect of venom was significantly less on normal breast cells than on cancer cells (P <0.05). Apoptotic cell death was significantly increased (P <0.05) in a dose dependent manner. Results of real time PCR confirmed the increase in apoptotic cell death due to venom treatment.
Conclusion: These data indicated that snake venom of Vipera raddei kurdistanica had anti-cancer properties through apoptosis cell death induction specifically in breast cancer cells. |
| first_indexed | 2024-12-12T08:33:18Z |
| format | Article |
| id | doaj.art-5a8f02a0083a451095a411ebd015e638 |
| institution | Directory Open Access Journal |
| issn | 1560-652X 2345-4040 |
| language | fas |
| last_indexed | 2024-12-12T08:33:18Z |
| publishDate | 2022-01-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj.art-5a8f02a0083a451095a411ebd015e6382022-12-22T00:31:02ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402022-01-01267110Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell LinesParsa Amirian0Kaveh Shahveisi1Mona Pazhouhi2Cyrus Jalili3 Student, Student Research Committee of Kermanshah University of Medical Sciences, Kermanshah, Iran. Assistant Professor, Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Research associate, Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran Professor, Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Background and Aim: Breast cancer is the second leading cause of death among women worldwide. Despite recent advances in cancer treatment, this disease remains one of the leading causes of death. Snake venom is a mixture of various molecules such as carbohydrates, nucleosides, amino acids, lipids, proteins and peptides. Previous studies have shown that the venom of some snakes have anti-cancer effects on human cell lines. In the present study, the effect of Vipera raddei kurdistanica venom on breast cell lines was investigated. Materials and Methods: The effect of increasing concentrations of snake venom on breast cells viability was investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and lactate dehydrogenase measurement. Apoptotic cell death was evaluated by fluorescent dye staining and DNA fragmentation assay. Changes in the expression levels of some apoptotic-related genes were investigated by using real time PCR. Data were analyzed by one-way ANOVA followed by Tukey's test. P <0.05 was considered significant. Results: After 24, 48, 72 and 96 hours treatment, cell viability was significantly decreased in a time and dose dependent manner (P <0.05). The effect of venom was significantly less on normal breast cells than on cancer cells (P <0.05). Apoptotic cell death was significantly increased (P <0.05) in a dose dependent manner. Results of real time PCR confirmed the increase in apoptotic cell death due to venom treatment. Conclusion: These data indicated that snake venom of Vipera raddei kurdistanica had anti-cancer properties through apoptosis cell death induction specifically in breast cancer cells.http://sjku.muk.ac.ir/article-1-5491-en.htmlapoptosisbreast cancercell culturevipera raddei kurdistanicasnake venom |
| spellingShingle | Parsa Amirian Kaveh Shahveisi Mona Pazhouhi Cyrus Jalili Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines مجله علمی دانشگاه علوم پزشکی کردستان apoptosis breast cancer cell culture vipera raddei kurdistanica snake venom |
| title | Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines |
| title_full | Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines |
| title_fullStr | Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines |
| title_full_unstemmed | Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines |
| title_short | Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines |
| title_sort | anti proliferative and apoptosis induction effect of iranian snake venom vipera raddei kurdistanica on human breast cancer mcf 7 and normal breast mcf 10a cell lines |
| topic | apoptosis breast cancer cell culture vipera raddei kurdistanica snake venom |
| url | http://sjku.muk.ac.ir/article-1-5491-en.html |
| work_keys_str_mv | AT parsaamirian antiproliferativeandapoptosisinductioneffectofiraniansnakevenomviperaraddeikurdistanicaonhumanbreastcancermcf7andnormalbreastmcf10acelllines AT kavehshahveisi antiproliferativeandapoptosisinductioneffectofiraniansnakevenomviperaraddeikurdistanicaonhumanbreastcancermcf7andnormalbreastmcf10acelllines AT monapazhouhi antiproliferativeandapoptosisinductioneffectofiraniansnakevenomviperaraddeikurdistanicaonhumanbreastcancermcf7andnormalbreastmcf10acelllines AT cyrusjalili antiproliferativeandapoptosisinductioneffectofiraniansnakevenomviperaraddeikurdistanicaonhumanbreastcancermcf7andnormalbreastmcf10acelllines |