Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification
Traditional microscopy provides only for a small set of magnifications using a finite set of microscope objectives. Here, a novel architecture is proposed for quantitative phase microscopy that requires only a simple adaptation of the traditional off-axis digital holographic microscope. The architec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/8/7/264 |
| _version_ | 1797526314742185984 |
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| author | Xin Fan John J. Healy Kevin O’Dwyer Julianna Winnik Bryan M. Hennelly |
| author_facet | Xin Fan John J. Healy Kevin O’Dwyer Julianna Winnik Bryan M. Hennelly |
| author_sort | Xin Fan |
| collection | DOAJ |
| description | Traditional microscopy provides only for a small set of magnifications using a finite set of microscope objectives. Here, a novel architecture is proposed for quantitative phase microscopy that requires only a simple adaptation of the traditional off-axis digital holographic microscope. The architecture has the key advantage of continuously variable magnification, resolution, and Field-of-View, by simply moving the sample. The method is based on combining the principles of traditional off-axis digital holographic microscopy and Gabor microscopy, which uses a diverging spherical wavefield for magnification. We present a proof-of-concept implementation and ray-tracing is used to model the magnification, Numerical Aperture, and Field-of-View as a function of sample position. Experimental results are presented using a micro-lens array and shortcomings of the method are highlighted for future work; in particular, the problem of aberration is highlighted, which results from imaging far from the focal plane of the infinity corrected microscope objective. |
| first_indexed | 2024-03-10T09:28:18Z |
| format | Article |
| id | doaj.art-5a8f8fde34944bff9b35a00c80616f5a |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-10T09:28:18Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-5a8f8fde34944bff9b35a00c80616f5a2023-11-22T04:42:18ZengMDPI AGPhotonics2304-67322021-07-018726410.3390/photonics8070264Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable MagnificationXin Fan0John J. Healy1Kevin O’Dwyer2Julianna Winnik3Bryan M. Hennelly4Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, ChinaSchool of Electrical and Electronic Engineering, University College Dublin, Dublin 4 Belfield, IrelandDepartment of Electronic Engineering, Maynooth University, W23 Maynooth, IrelandInstitute of Micromechanics and Photonics, Warsaw University of Technology, 8 Sw. Andrzeja Boboli Street, 02-525 Warsaw, PolandDepartment of Electronic Engineering, Maynooth University, W23 Maynooth, IrelandTraditional microscopy provides only for a small set of magnifications using a finite set of microscope objectives. Here, a novel architecture is proposed for quantitative phase microscopy that requires only a simple adaptation of the traditional off-axis digital holographic microscope. The architecture has the key advantage of continuously variable magnification, resolution, and Field-of-View, by simply moving the sample. The method is based on combining the principles of traditional off-axis digital holographic microscopy and Gabor microscopy, which uses a diverging spherical wavefield for magnification. We present a proof-of-concept implementation and ray-tracing is used to model the magnification, Numerical Aperture, and Field-of-View as a function of sample position. Experimental results are presented using a micro-lens array and shortcomings of the method are highlighted for future work; in particular, the problem of aberration is highlighted, which results from imaging far from the focal plane of the infinity corrected microscope objective.https://www.mdpi.com/2304-6732/8/7/264digital holographic microscopydigital inline holographic microscopyvariable magnificationoff-axis holography |
| spellingShingle | Xin Fan John J. Healy Kevin O’Dwyer Julianna Winnik Bryan M. Hennelly Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification Photonics digital holographic microscopy digital inline holographic microscopy variable magnification off-axis holography |
| title | Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification |
| title_full | Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification |
| title_fullStr | Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification |
| title_full_unstemmed | Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification |
| title_short | Adaptation of the Standard Off-Axis Digital Holographic Microscope to Achieve Variable Magnification |
| title_sort | adaptation of the standard off axis digital holographic microscope to achieve variable magnification |
| topic | digital holographic microscopy digital inline holographic microscopy variable magnification off-axis holography |
| url | https://www.mdpi.com/2304-6732/8/7/264 |
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