In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula
The gastrointestinal microbiome has a range of roles in the host, including the production of beneficial fermentation end products such as butyrate, which are typically associated with fermentation of plant fibres. However, domestic cats are obligate carnivores and do not require carbohydrates. It h...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/4/498 |
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| author | Christina F. Butowski David G. Thomas Nick J. Cave Emma N. Bermingham Douglas I. Rosendale Shen-Yan Hea Halina M. Stoklosinski Wayne Young |
| author_facet | Christina F. Butowski David G. Thomas Nick J. Cave Emma N. Bermingham Douglas I. Rosendale Shen-Yan Hea Halina M. Stoklosinski Wayne Young |
| author_sort | Christina F. Butowski |
| collection | DOAJ |
| description | The gastrointestinal microbiome has a range of roles in the host, including the production of beneficial fermentation end products such as butyrate, which are typically associated with fermentation of plant fibres. However, domestic cats are obligate carnivores and do not require carbohydrates. It has been hypothesised that in the wild, collagenous parts of prey—the so-called animal-derived fermentable substrates (ADFS) such as tendons and cartilage—may be fermented by the cat’s gastrointestinal microbiome. However, little research has been conducted on ADFS in the domestic cat. Faecal inoculum was obtained from domestic cats either consuming a high carbohydrate (protein:fat:carbohydrate ratio of 35:20:28 (% dry matter basis)) or high protein (protein:fat:carbohydrate ratio of 75:19:1 (% dry matter basis)) diet. ADFS (hydrolysed collagen, cat hair, and cartilage) were used in a series of static in vitro digestions and fermentations. Concentrations of organic acids and ammonia were measured after 24 h of fermentation, and the culture community of microbes was characterised. The type of inoculum used affected the fermentation profile produced by the ADFS. Butyrate concentrations were highest when hydrolysed collagen was fermented with high protein inoculum (<i>p</i> < 0.05). In contrast, butyrate was not detectable when hydrolysed collagen was fermented in high carbohydrate inoculum (<i>p</i> < 0.05). The microbiome of the domestic cat may be able to ferment ADFS to provide beneficial concentrations of butyrate. |
| first_indexed | 2024-03-09T22:47:20Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:47:20Z |
| publishDate | 2022-02-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-5a90aa5dfc9d4743ba1e57d2bc71715e2023-11-23T18:26:08ZengMDPI AGAnimals2076-26152022-02-0112449810.3390/ani12040498In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal InoculaChristina F. Butowski0David G. Thomas1Nick J. Cave2Emma N. Bermingham3Douglas I. Rosendale4Shen-Yan Hea5Halina M. Stoklosinski6Wayne Young7Smart Foods, AgResearch Limited, Te Ohu Rangahau Kai, Palmerston North 4474, New ZealandCentre for Feline Nutrition, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4474, New ZealandSmart Foods, AgResearch Limited, Te Ohu Rangahau Kai, Palmerston North 4474, New ZealandAnagenix, Auckland 1052, New ZealandDigital Agriculture, AgResearch Limited, Grasslands, Palmerston North 4410, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Fitzherbert Science Centre, Palmerston North 4474, New ZealandSmart Foods, AgResearch Limited, Te Ohu Rangahau Kai, Palmerston North 4474, New ZealandThe gastrointestinal microbiome has a range of roles in the host, including the production of beneficial fermentation end products such as butyrate, which are typically associated with fermentation of plant fibres. However, domestic cats are obligate carnivores and do not require carbohydrates. It has been hypothesised that in the wild, collagenous parts of prey—the so-called animal-derived fermentable substrates (ADFS) such as tendons and cartilage—may be fermented by the cat’s gastrointestinal microbiome. However, little research has been conducted on ADFS in the domestic cat. Faecal inoculum was obtained from domestic cats either consuming a high carbohydrate (protein:fat:carbohydrate ratio of 35:20:28 (% dry matter basis)) or high protein (protein:fat:carbohydrate ratio of 75:19:1 (% dry matter basis)) diet. ADFS (hydrolysed collagen, cat hair, and cartilage) were used in a series of static in vitro digestions and fermentations. Concentrations of organic acids and ammonia were measured after 24 h of fermentation, and the culture community of microbes was characterised. The type of inoculum used affected the fermentation profile produced by the ADFS. Butyrate concentrations were highest when hydrolysed collagen was fermented with high protein inoculum (<i>p</i> < 0.05). In contrast, butyrate was not detectable when hydrolysed collagen was fermented in high carbohydrate inoculum (<i>p</i> < 0.05). The microbiome of the domestic cat may be able to ferment ADFS to provide beneficial concentrations of butyrate.https://www.mdpi.com/2076-2615/12/4/498in vitrofermentationbutyratefelinecollagenfaecal donor |
| spellingShingle | Christina F. Butowski David G. Thomas Nick J. Cave Emma N. Bermingham Douglas I. Rosendale Shen-Yan Hea Halina M. Stoklosinski Wayne Young In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula Animals in vitro fermentation butyrate feline collagen faecal donor |
| title | In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula |
| title_full | In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula |
| title_fullStr | In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula |
| title_full_unstemmed | In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula |
| title_short | In Vitro Assessment of Hydrolysed Collagen Fermentation Using Domestic Cat (<i>Felis catus</i>) Faecal Inocula |
| title_sort | in vitro assessment of hydrolysed collagen fermentation using domestic cat i felis catus i faecal inocula |
| topic | in vitro fermentation butyrate feline collagen faecal donor |
| url | https://www.mdpi.com/2076-2615/12/4/498 |
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