3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i>
Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterial...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/18/2/123 |
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| author | Marcin Wysokowski Tomasz Machałowski Iaroslav Petrenko Christian Schimpf David Rafaja Roberta Galli Jerzy Ziętek Snežana Pantović Alona Voronkina Valentine Kovalchuk Viatcheslav N. Ivanenko Bert W. Hoeksema Cristina Diaz Yuliya Khrunyk Allison L. Stelling Marco Giovine Teofil Jesionowski Hermann Ehrlich |
| author_facet | Marcin Wysokowski Tomasz Machałowski Iaroslav Petrenko Christian Schimpf David Rafaja Roberta Galli Jerzy Ziętek Snežana Pantović Alona Voronkina Valentine Kovalchuk Viatcheslav N. Ivanenko Bert W. Hoeksema Cristina Diaz Yuliya Khrunyk Allison L. Stelling Marco Giovine Teofil Jesionowski Hermann Ehrlich |
| author_sort | Marcin Wysokowski |
| collection | DOAJ |
| description | Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge <i>Aplysin aarcheri</i> and the terrestrial snail <i>Cornu aspersum</i> were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO<sub>3</sub> amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure. |
| first_indexed | 2024-04-13T08:07:58Z |
| format | Article |
| id | doaj.art-5a90d167782a46f7baacbc89e04ed82c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T08:07:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-5a90d167782a46f7baacbc89e04ed82c2022-12-22T02:55:06ZengMDPI AGMarine Drugs1660-33972020-02-0118212310.3390/md18020123md180201233D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i>Marcin Wysokowski0Tomasz Machałowski1Iaroslav Petrenko2Christian Schimpf3David Rafaja4Roberta Galli5Jerzy Ziętek6Snežana Pantović7Alona Voronkina8Valentine Kovalchuk9Viatcheslav N. Ivanenko10Bert W. Hoeksema11Cristina Diaz12Yuliya Khrunyk13Allison L. Stelling14Marco Giovine15Teofil Jesionowski16Hermann Ehrlich17Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, PolandFaculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, PolandInstitute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav-Zeuner str. 3, 09599 Freiberg, GermanyInstitute of Materials Science, TU Bergakademie Freiberg, 09599 Freiberg, GermanyInstitute of Materials Science, TU Bergakademie Freiberg, 09599 Freiberg, GermanyClinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyFaculty of Veterinary Medicine, Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, Głęboka 30, 20612 Lublin, PolandFaculty of Medicine, University of Montenegro, Kruševac bb, 81000 Podgorica, MontenegroDepartment of Pharmacy, National Pirogov Memorial Medical University, 21018 Vinnitsa, UkraineDepartment of Microbiology, National Pirogov Memorial Medical University, 21018 Vinnitsa, UkraineDepartment of Invertebrate Zoology, Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, RussiaTaxonomy and Systematics Group, Naturalis Biodiversity Center, 2333CR Leiden, The NetherlandsHarbor Branch Oceanographic Institute, Florida Atlantic University, 5600 Old Dixie Hwy, Fort Pierce, FL 34946, USADepartment of Heat Treatment and Physics of Metal, Ural Federal University, Mira Str. 19, 620002 Ekaterinburg, RussiaDepartment of Biochemistry, Duke University Medical School, Durham, NC 27708, USADepartment of Sciences of Earth, Environment and Life, University of Genoa, Corso Europa 26, 16132 Genova, ItalyFaculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, PolandInstitute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Gustav-Zeuner str. 3, 09599 Freiberg, GermanyStructure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge <i>Aplysin aarcheri</i> and the terrestrial snail <i>Cornu aspersum</i> were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO<sub>3</sub> amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure.https://www.mdpi.com/1660-3397/18/2/123chitinscaffoldspongeshemocyteshemolymphbiomineralizationcalcite |
| spellingShingle | Marcin Wysokowski Tomasz Machałowski Iaroslav Petrenko Christian Schimpf David Rafaja Roberta Galli Jerzy Ziętek Snežana Pantović Alona Voronkina Valentine Kovalchuk Viatcheslav N. Ivanenko Bert W. Hoeksema Cristina Diaz Yuliya Khrunyk Allison L. Stelling Marco Giovine Teofil Jesionowski Hermann Ehrlich 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> Marine Drugs chitin scaffold sponges hemocytes hemolymph biomineralization calcite |
| title | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> |
| title_full | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> |
| title_fullStr | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> |
| title_full_unstemmed | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> |
| title_short | 3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization <i>Ex-Vivo</i> |
| title_sort | 3d chitin scaffolds of marine demosponge origin for biomimetic mollusk hemolymph associated biomineralization i ex vivo i |
| topic | chitin scaffold sponges hemocytes hemolymph biomineralization calcite |
| url | https://www.mdpi.com/1660-3397/18/2/123 |
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