Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice
Delta-6 desaturase-null mice (−/−) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The −/− males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-02-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520305411 |
| _version_ | 1818600983923523584 |
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| author | Manuel Roqueta-Rivera Chad K. Stroud Wanda M. Haschek Sandeep J. Akare Mariangela Segre Richard S. Brush Martin-Paul Agbaga Robert E. Anderson Rex A. Hess Manabu T. Nakamura |
| author_facet | Manuel Roqueta-Rivera Chad K. Stroud Wanda M. Haschek Sandeep J. Akare Mariangela Segre Richard S. Brush Martin-Paul Agbaga Robert E. Anderson Rex A. Hess Manabu T. Nakamura |
| author_sort | Manuel Roqueta-Rivera |
| collection | DOAJ |
| description | Delta-6 desaturase-null mice (−/−) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The −/− males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type (+/+) and −/− males at weaning until 16 weeks of age (n = 3–5). A breeding success rate of DHA-supplemented −/− was comparable to +/+. DHA-fed −/− showed normal sperm counts and spermiogenesis. Dietary AA was less effective in restoring fertility, sperm count, and spermiogenesis than DHA. Testis fatty acid analysis showed restored DHA in DHA-fed −/−, but DPAn6 remained depleted. In AA-fed −/−, AA was restored at the +/+ level, and 22:4n6, an AA elongated product, accumulated in testis. Cholesta-3,5-diene was present in testis of +/+ and DHA-fed −/−, whereas it diminished in −/− and AA-fed −/−, suggesting impaired sterol metabolism in these groups. Expression of spermiogenesis marker genes was largely normal in all groups. In conclusion, DHA was capable of restoring all observed impairment in male reproduction, whereas 22:4n6 formed from dietary AA may act as an inferior substitute for DHA. |
| first_indexed | 2024-12-16T12:44:10Z |
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| id | doaj.art-5a93a242e46849a9a75a02904c5a7b2f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T12:44:10Z |
| publishDate | 2010-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5a93a242e46849a9a75a02904c5a7b2f2022-12-21T22:31:19ZengElsevierJournal of Lipid Research0022-22752010-02-01512360367Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null miceManuel Roqueta-Rivera0Chad K. Stroud1Wanda M. Haschek2Sandeep J. Akare3Mariangela Segre4Richard S. Brush5Martin-Paul Agbaga6Robert E. Anderson7Rex A. Hess8Manabu T. Nakamura9Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801Departments of Cell Biology and Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104Departments of Cell Biology and Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104Departments of Cell Biology and Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104Department of Veterinary Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801To whom correspondence should be addressed; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801Delta-6 desaturase-null mice (−/−) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The −/− males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type (+/+) and −/− males at weaning until 16 weeks of age (n = 3–5). A breeding success rate of DHA-supplemented −/− was comparable to +/+. DHA-fed −/− showed normal sperm counts and spermiogenesis. Dietary AA was less effective in restoring fertility, sperm count, and spermiogenesis than DHA. Testis fatty acid analysis showed restored DHA in DHA-fed −/−, but DPAn6 remained depleted. In AA-fed −/−, AA was restored at the +/+ level, and 22:4n6, an AA elongated product, accumulated in testis. Cholesta-3,5-diene was present in testis of +/+ and DHA-fed −/−, whereas it diminished in −/− and AA-fed −/−, suggesting impaired sterol metabolism in these groups. Expression of spermiogenesis marker genes was largely normal in all groups. In conclusion, DHA was capable of restoring all observed impairment in male reproduction, whereas 22:4n6 formed from dietary AA may act as an inferior substitute for DHA.http://www.sciencedirect.com/science/article/pii/S0022227520305411essential fatty acidsarachidonic acidhighly unsaturated fatty acidsvery-long-chain polyunsaturated fatty acidschoelsta-3,5-dienemale reproduction |
| spellingShingle | Manuel Roqueta-Rivera Chad K. Stroud Wanda M. Haschek Sandeep J. Akare Mariangela Segre Richard S. Brush Martin-Paul Agbaga Robert E. Anderson Rex A. Hess Manabu T. Nakamura Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice Journal of Lipid Research essential fatty acids arachidonic acid highly unsaturated fatty acids very-long-chain polyunsaturated fatty acids choelsta-3,5-diene male reproduction |
| title | Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice |
| title_full | Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice |
| title_fullStr | Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice |
| title_full_unstemmed | Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice |
| title_short | Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice |
| title_sort | docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta 6 desaturase null mice |
| topic | essential fatty acids arachidonic acid highly unsaturated fatty acids very-long-chain polyunsaturated fatty acids choelsta-3,5-diene male reproduction |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520305411 |
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