Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway
Aims: Glioma is the most malignant brain tumor that has the ability to migrate and invade the CNS. In this study, we investigated the signaling mechanism of caffeine on the migration of glioma cells. Methods: The effect of caffeine on cell migration was evaluated using Transwell and wound healing as...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2014-06-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/362966 |
| _version_ | 1817999790196129792 |
|---|---|
| author | Ying Chen Wei-Chung Chou You-Ming Ding Ya-Chieh Wu |
| author_facet | Ying Chen Wei-Chung Chou You-Ming Ding Ya-Chieh Wu |
| author_sort | Ying Chen |
| collection | DOAJ |
| description | Aims: Glioma is the most malignant brain tumor that has the ability to migrate and invade the CNS. In this study, we investigated the signaling mechanism of caffeine on the migration of glioma cells. Methods: The effect of caffeine on cell migration was evaluated using Transwell and wound healing assays. The expression of the focal adhesion complex as it related to cell migration was assayed using Western blotting and immunostaining. Results: Caffeine decreased the migration of rat C6 and human U87MG glioma cells and down-regulated the expression of phosphorylated focal adhesion kinase (p-FAK) and p-paxillin. Caffeine also decreased p-FAK staining at the edge of glioma cells and disassembled actin stress fibers. Additionally, caffeine elevated expression of phosphorylated myosin light chain (p-MLC), an effect that could be blocked by Y27632, a rho-associated protein kinase (ROCK) inhibitor, but not myosin light chain kinase inhibitor, ML-7. Y27632 also inhibited the caffeine-reduced expression of p-FAK and p-paxillin as well as cell migration. Conclusion: Caffeine decreased the migration of glioma cell through the ROCK-focal adhesion complex pathway; this mechanism may be useful as part of clinical therapy in the future. |
| first_indexed | 2024-04-14T03:13:45Z |
| format | Article |
| id | doaj.art-5a95d0fdd83a407891163706a89c7a8d |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-14T03:13:45Z |
| publishDate | 2014-06-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-5a95d0fdd83a407891163706a89c7a8d2022-12-22T02:15:32ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-06-013361888189810.1159/000362966362966Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK PathwayYing ChenWei-Chung ChouYou-Ming DingYa-Chieh WuAims: Glioma is the most malignant brain tumor that has the ability to migrate and invade the CNS. In this study, we investigated the signaling mechanism of caffeine on the migration of glioma cells. Methods: The effect of caffeine on cell migration was evaluated using Transwell and wound healing assays. The expression of the focal adhesion complex as it related to cell migration was assayed using Western blotting and immunostaining. Results: Caffeine decreased the migration of rat C6 and human U87MG glioma cells and down-regulated the expression of phosphorylated focal adhesion kinase (p-FAK) and p-paxillin. Caffeine also decreased p-FAK staining at the edge of glioma cells and disassembled actin stress fibers. Additionally, caffeine elevated expression of phosphorylated myosin light chain (p-MLC), an effect that could be blocked by Y27632, a rho-associated protein kinase (ROCK) inhibitor, but not myosin light chain kinase inhibitor, ML-7. Y27632 also inhibited the caffeine-reduced expression of p-FAK and p-paxillin as well as cell migration. Conclusion: Caffeine decreased the migration of glioma cell through the ROCK-focal adhesion complex pathway; this mechanism may be useful as part of clinical therapy in the future.http://www.karger.com/Article/FullText/362966CaffeineRho associated kinaseFocal adhesion kinaseGlioma |
| spellingShingle | Ying Chen Wei-Chung Chou You-Ming Ding Ya-Chieh Wu Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway Cellular Physiology and Biochemistry Caffeine Rho associated kinase Focal adhesion kinase Glioma |
| title | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
| title_full | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
| title_fullStr | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
| title_full_unstemmed | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
| title_short | Caffeine Inhibits Migration in Glioma Cells through the ROCK-FAK Pathway |
| title_sort | caffeine inhibits migration in glioma cells through the rock fak pathway |
| topic | Caffeine Rho associated kinase Focal adhesion kinase Glioma |
| url | http://www.karger.com/Article/FullText/362966 |
| work_keys_str_mv | AT yingchen caffeineinhibitsmigrationingliomacellsthroughtherockfakpathway AT weichungchou caffeineinhibitsmigrationingliomacellsthroughtherockfakpathway AT youmingding caffeineinhibitsmigrationingliomacellsthroughtherockfakpathway AT yachiehwu caffeineinhibitsmigrationingliomacellsthroughtherockfakpathway |