Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin
Caloric restriction (CR) represents a powerful intervention for extending healthspan and lifespan in several animal models, from yeast to primates. Additionally, in humans, CR has been found to induce cardiometabolic adaptations associated with improved health. In this study, we evaluated in an aged...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/12/532 |
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| author | Daniele La Russa Alessandro Marrone Maurizio Mandalà Rachele Macirella Daniela Pellegrino |
| author_facet | Daniele La Russa Alessandro Marrone Maurizio Mandalà Rachele Macirella Daniela Pellegrino |
| author_sort | Daniele La Russa |
| collection | DOAJ |
| description | Caloric restriction (CR) represents a powerful intervention for extending healthspan and lifespan in several animal models, from yeast to primates. Additionally, in humans, CR has been found to induce cardiometabolic adaptations associated with improved health. In this study, we evaluated in an aged and obese rat model the effect of long-term (6 months) caloric restriction (−40%) on the oxidative/inflammatory balance in order to investigate the underlining mechanisms. In plasma, we analyzed the oxidative balance by photometric tests and the adiponectin/tumor necrosis factor-α-induced gene/protein 6 (TSG-6) levels by Western blot analysis. In the white adipose tissue, we examined the protein levels of AdipoR1, pAMPK, NFκB, NRF-2, and glutathione S-tranferase P1 by Western blot analysis. Our results clearly showed that caloric restriction significantly improves the plasmatic oxidative/inflammatory balance in parallel with a major increase in circulating adiponectin levels. Additionally, at the level of adipose tissue, we found a positive modulation of both anti-inflammatory and antioxidant pathways. These adaptations, induced by caloric restriction, with the achievement of normal weight, suggest that inflammatory and redox imbalance in obese aged rats appear to be more linked to obesity than to aging. |
| first_indexed | 2024-03-10T14:35:50Z |
| format | Article |
| id | doaj.art-5a9784b541e74f1b893081e59f4add11 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T14:35:50Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-5a9784b541e74f1b893081e59f4add112023-11-20T22:12:52ZengMDPI AGBiomedicines2227-90592020-11-0181253210.3390/biomedicines8120532Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of AdiponectinDaniele La Russa0Alessandro Marrone1Maurizio Mandalà2Rachele Macirella3Daniela Pellegrino4Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyLARSO (Analysis and Research on Oxidative Stress Laboratory), University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Biology, Ecology and Earth Sciences, University of Calabria, 87036 Rende, ItalyLARSO (Analysis and Research on Oxidative Stress Laboratory), University of Calabria, 87036 Rende, ItalyCaloric restriction (CR) represents a powerful intervention for extending healthspan and lifespan in several animal models, from yeast to primates. Additionally, in humans, CR has been found to induce cardiometabolic adaptations associated with improved health. In this study, we evaluated in an aged and obese rat model the effect of long-term (6 months) caloric restriction (−40%) on the oxidative/inflammatory balance in order to investigate the underlining mechanisms. In plasma, we analyzed the oxidative balance by photometric tests and the adiponectin/tumor necrosis factor-α-induced gene/protein 6 (TSG-6) levels by Western blot analysis. In the white adipose tissue, we examined the protein levels of AdipoR1, pAMPK, NFκB, NRF-2, and glutathione S-tranferase P1 by Western blot analysis. Our results clearly showed that caloric restriction significantly improves the plasmatic oxidative/inflammatory balance in parallel with a major increase in circulating adiponectin levels. Additionally, at the level of adipose tissue, we found a positive modulation of both anti-inflammatory and antioxidant pathways. These adaptations, induced by caloric restriction, with the achievement of normal weight, suggest that inflammatory and redox imbalance in obese aged rats appear to be more linked to obesity than to aging.https://www.mdpi.com/2227-9059/8/12/532caloric restrictioninflammationoxidative balanceadiponectinplasmawhite adipose tissue |
| spellingShingle | Daniele La Russa Alessandro Marrone Maurizio Mandalà Rachele Macirella Daniela Pellegrino Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin Biomedicines caloric restriction inflammation oxidative balance adiponectin plasma white adipose tissue |
| title | Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin |
| title_full | Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin |
| title_fullStr | Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin |
| title_full_unstemmed | Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin |
| title_short | Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese Rat Model: The Role of Adiponectin |
| title_sort | antioxidant anti inflammatory effects of caloric restriction in an aged and obese rat model the role of adiponectin |
| topic | caloric restriction inflammation oxidative balance adiponectin plasma white adipose tissue |
| url | https://www.mdpi.com/2227-9059/8/12/532 |
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