Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing
Endometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic too...
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MDPI AG
2021-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/1/88 |
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| author | Alba Martí Anna Serrano Josep Pastor Teresa Rigau Ugné Petkevičiuté Maria Àngels Calvo Esteban Leonardo Arosemena Aida Yuste David Prandi Adrià Aguilar Maria Montserrat Rivera del Alamo |
| author_facet | Alba Martí Anna Serrano Josep Pastor Teresa Rigau Ugné Petkevičiuté Maria Àngels Calvo Esteban Leonardo Arosemena Aida Yuste David Prandi Adrià Aguilar Maria Montserrat Rivera del Alamo |
| author_sort | Alba Martí |
| collection | DOAJ |
| description | Endometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic tool for evaluating the health status of the endometrium in queens. Fifty domestic shorthair queens were included and divided into two cytological diagnostic technique groups, the uterine lavage (UL; n = 28) and uterine swabbing (US; n = 22) groups. Cytological results were compared with histopathological and bacteriological information. Changes in the histopathological patterns were also evaluated and compared with progesterone levels to confirm previous published data. Furthermore, the results from both cytological sampling methods were compared to evaluate the utility of each method. Endometritis was ruled out in all queens by means of histology and microbiology. Leukocyte counts and red blood cell/endometrial cell ratios were significantly higher in US than UL samples. Additionally, UL sampling is less affected by blood contamination and cells are better preserved. The combination of endometrial cytology and uterine culture might be useful for evaluating the endometrial characteristics in queens. The UL evaluation method is more representative of the actual endometrial status than the US technique. |
| first_indexed | 2024-03-10T13:27:58Z |
| format | Article |
| id | doaj.art-5a9a43a98d94413fbe2d0f0a3d6ebf43 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:27:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5a9a43a98d94413fbe2d0f0a3d6ebf432023-11-21T08:30:27ZengMDPI AGAnimals2076-26152021-01-011118810.3390/ani11010088Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine SwabbingAlba Martí0Anna Serrano1Josep Pastor2Teresa Rigau3Ugné Petkevičiuté4Maria Àngels Calvo5Esteban Leonardo Arosemena6Aida Yuste7David Prandi8Adrià Aguilar9Maria Montserrat Rivera del Alamo10Department of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Anatomy, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Anatomy, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Anatomy, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainEndometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic tool for evaluating the health status of the endometrium in queens. Fifty domestic shorthair queens were included and divided into two cytological diagnostic technique groups, the uterine lavage (UL; n = 28) and uterine swabbing (US; n = 22) groups. Cytological results were compared with histopathological and bacteriological information. Changes in the histopathological patterns were also evaluated and compared with progesterone levels to confirm previous published data. Furthermore, the results from both cytological sampling methods were compared to evaluate the utility of each method. Endometritis was ruled out in all queens by means of histology and microbiology. Leukocyte counts and red blood cell/endometrial cell ratios were significantly higher in US than UL samples. Additionally, UL sampling is less affected by blood contamination and cells are better preserved. The combination of endometrial cytology and uterine culture might be useful for evaluating the endometrial characteristics in queens. The UL evaluation method is more representative of the actual endometrial status than the US technique.https://www.mdpi.com/2076-2615/11/1/88uteruscytologypolymorphonuclear neutrophilqueen |
| spellingShingle | Alba Martí Anna Serrano Josep Pastor Teresa Rigau Ugné Petkevičiuté Maria Àngels Calvo Esteban Leonardo Arosemena Aida Yuste David Prandi Adrià Aguilar Maria Montserrat Rivera del Alamo Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing Animals uterus cytology polymorphonuclear neutrophil queen |
| title | Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing |
| title_full | Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing |
| title_fullStr | Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing |
| title_full_unstemmed | Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing |
| title_short | Endometrial Status in Queens Evaluated by Histopathology Findings and Two Cytological Techniques: Low-Volume Uterine Lavage and Uterine Swabbing |
| title_sort | endometrial status in queens evaluated by histopathology findings and two cytological techniques low volume uterine lavage and uterine swabbing |
| topic | uterus cytology polymorphonuclear neutrophil queen |
| url | https://www.mdpi.com/2076-2615/11/1/88 |
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