N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm
Abstract Intraductal papillary mucinous neoplasms (IPMNs), often found incidentally, are potentially malignant cystic tumors of the pancreas. Due to the precancerous nature, IPMNs lacking malignant features should be kept on surveillance. The follow-up relies on magnetic resonance imaging, which has...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39220-4 |
| _version_ | 1797559969398128640 |
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| author | Heini Nieminen Pirjo Nummela Tero Satomaa Annamari Heiskanen Jukka O. Hiltunen Tuomas Kaprio Hanna Seppänen Jaana Hagström Harri Mustonen Ari Ristimäki Caj Haglund |
| author_facet | Heini Nieminen Pirjo Nummela Tero Satomaa Annamari Heiskanen Jukka O. Hiltunen Tuomas Kaprio Hanna Seppänen Jaana Hagström Harri Mustonen Ari Ristimäki Caj Haglund |
| author_sort | Heini Nieminen |
| collection | DOAJ |
| description | Abstract Intraductal papillary mucinous neoplasms (IPMNs), often found incidentally, are potentially malignant cystic tumors of the pancreas. Due to the precancerous nature, IPMNs lacking malignant features should be kept on surveillance. The follow-up relies on magnetic resonance imaging, which has a limited accuracy to define the high-risk patients. New diagnostic methods are thus needed to recognize IPMNs with malignant potential. Here, aberrantly expressed glycans constitute a promising new area of research. We compared the N-glycan profiles of non-invasive IPMN tissues (n = 10) and invasive IPMN tissues (n = 10) to those of non-neoplastic pancreatic controls (n = 5) by matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. Both IPMN subgroups showed increased abundance of neutral composition H4N4 and decrease in H3N5F1, increase in sialylation, and decrease in sulfation, as compared to the controls. Furthermore, invasive IPMN showed an increase in terminal N-acetylhexosamine containing structure H4N5, and increase in acidic complex-type glycans, but decrease in their complex fucosylation and sulfation, as compared to the controls. In conclusion, the N-glycan profiles differed between healthy pancreatic tissue and non-invasive and invasive IPMNs. The unique glycans expressed in invasive IPMNs may offer interesting new options for diagnostics. |
| first_indexed | 2024-03-10T17:52:46Z |
| format | Article |
| id | doaj.art-5a9bac3523a54ae496990b9cd1e79283 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:52:46Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5a9bac3523a54ae496990b9cd1e792832023-11-20T09:17:14ZengNature PortfolioScientific Reports2045-23222023-08-0113111210.1038/s41598-023-39220-4N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasmHeini Nieminen0Pirjo Nummela1Tero Satomaa2Annamari Heiskanen3Jukka O. Hiltunen4Tuomas Kaprio5Hanna Seppänen6Jaana Hagström7Harri Mustonen8Ari Ristimäki9Caj Haglund10Department of Surgery, University of Helsinki and Helsinki University HospitalApplied Tumor Genomics Research Program, Research Programs Unit, University of Helsinki and Helsinki University HospitalGlykos Finland LtdGlykos Finland LtdGlykos Finland LtdDepartment of Surgery, University of Helsinki and Helsinki University HospitalDepartment of Surgery, University of Helsinki and Helsinki University HospitalResearch Programs Unit, Translational Cancer Medicine Research Program, University of HelsinkiDepartment of Surgery, University of Helsinki and Helsinki University HospitalApplied Tumor Genomics Research Program, Research Programs Unit, University of Helsinki and Helsinki University HospitalDepartment of Surgery, University of Helsinki and Helsinki University HospitalAbstract Intraductal papillary mucinous neoplasms (IPMNs), often found incidentally, are potentially malignant cystic tumors of the pancreas. Due to the precancerous nature, IPMNs lacking malignant features should be kept on surveillance. The follow-up relies on magnetic resonance imaging, which has a limited accuracy to define the high-risk patients. New diagnostic methods are thus needed to recognize IPMNs with malignant potential. Here, aberrantly expressed glycans constitute a promising new area of research. We compared the N-glycan profiles of non-invasive IPMN tissues (n = 10) and invasive IPMN tissues (n = 10) to those of non-neoplastic pancreatic controls (n = 5) by matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. Both IPMN subgroups showed increased abundance of neutral composition H4N4 and decrease in H3N5F1, increase in sialylation, and decrease in sulfation, as compared to the controls. Furthermore, invasive IPMN showed an increase in terminal N-acetylhexosamine containing structure H4N5, and increase in acidic complex-type glycans, but decrease in their complex fucosylation and sulfation, as compared to the controls. In conclusion, the N-glycan profiles differed between healthy pancreatic tissue and non-invasive and invasive IPMNs. The unique glycans expressed in invasive IPMNs may offer interesting new options for diagnostics.https://doi.org/10.1038/s41598-023-39220-4 |
| spellingShingle | Heini Nieminen Pirjo Nummela Tero Satomaa Annamari Heiskanen Jukka O. Hiltunen Tuomas Kaprio Hanna Seppänen Jaana Hagström Harri Mustonen Ari Ristimäki Caj Haglund N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm Scientific Reports |
| title | N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm |
| title_full | N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm |
| title_fullStr | N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm |
| title_full_unstemmed | N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm |
| title_short | N-glycosylation in non-invasive and invasive intraductal papillary mucinous neoplasm |
| title_sort | n glycosylation in non invasive and invasive intraductal papillary mucinous neoplasm |
| url | https://doi.org/10.1038/s41598-023-39220-4 |
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