A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells
<p>Abstract</p> <p>Background</p> <p>The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions in other tissues, th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-11-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/10/543 |
| _version_ | 1811285679410249728 |
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| author | Giraudi Pablo Roncaglia Paola Foti Rossana Bellarosa Cristina Calligaris Raffaella Krmac Helena Tiribelli Claudio Gustincich Stefano |
| author_facet | Giraudi Pablo Roncaglia Paola Foti Rossana Bellarosa Cristina Calligaris Raffaella Krmac Helena Tiribelli Claudio Gustincich Stefano |
| author_sort | Giraudi Pablo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions in other tissues, the basic mechanisms of neurotoxicity have not yet been fully clarified. While cells can accumulate UCB by passive diffusion, cell protection may involve multiple mechanisms including the extrusion of the pigment as well as pro-survival homeostatic responses that are still unknown.</p> <p>Results</p> <p>Transcriptome changes induced by UCB exposure in SH-SY5Y neuroblastoma cell line were examined by high density oligonucleotide microarrays. Two-hundred and thirty genes were induced after 24 hours. A Gene Ontology (GO) analysis showed that at least 50 genes were directly involved in the endoplasmic reticulum (ER) stress response. Validation of selected ER stress genes is shown by quantitative RT-PCR. Analysis of <it>XBP1 </it>splicing and DDIT3/CHOP subcellular localization is presented.</p> <p>Conclusion</p> <p>These results show for the first time that UCB exposure induces ER stress response as major intracellular homeostasis in surviving neuroblastoma cells in vitro.</p> |
| first_indexed | 2024-04-13T02:47:38Z |
| format | Article |
| id | doaj.art-5a9d27fccfc64fab9bf1e62ac230576e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T02:47:38Z |
| publishDate | 2009-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-5a9d27fccfc64fab9bf1e62ac230576e2022-12-22T03:05:57ZengBMCBMC Genomics1471-21642009-11-0110154310.1186/1471-2164-10-543A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cellsGiraudi PabloRoncaglia PaolaFoti RossanaBellarosa CristinaCalligaris RaffaellaKrmac HelenaTiribelli ClaudioGustincich Stefano<p>Abstract</p> <p>Background</p> <p>The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions in other tissues, the basic mechanisms of neurotoxicity have not yet been fully clarified. While cells can accumulate UCB by passive diffusion, cell protection may involve multiple mechanisms including the extrusion of the pigment as well as pro-survival homeostatic responses that are still unknown.</p> <p>Results</p> <p>Transcriptome changes induced by UCB exposure in SH-SY5Y neuroblastoma cell line were examined by high density oligonucleotide microarrays. Two-hundred and thirty genes were induced after 24 hours. A Gene Ontology (GO) analysis showed that at least 50 genes were directly involved in the endoplasmic reticulum (ER) stress response. Validation of selected ER stress genes is shown by quantitative RT-PCR. Analysis of <it>XBP1 </it>splicing and DDIT3/CHOP subcellular localization is presented.</p> <p>Conclusion</p> <p>These results show for the first time that UCB exposure induces ER stress response as major intracellular homeostasis in surviving neuroblastoma cells in vitro.</p>http://www.biomedcentral.com/1471-2164/10/543 |
| spellingShingle | Giraudi Pablo Roncaglia Paola Foti Rossana Bellarosa Cristina Calligaris Raffaella Krmac Helena Tiribelli Claudio Gustincich Stefano A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells BMC Genomics |
| title | A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells |
| title_full | A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells |
| title_fullStr | A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells |
| title_full_unstemmed | A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells |
| title_short | A transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells |
| title_sort | transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma sh sy5y cells |
| url | http://www.biomedcentral.com/1471-2164/10/543 |
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