Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients
<p>Abstract</p> <p>Background</p> <p>Pathogenesis of liver damage in patients with HIV and HCV co-infection is complex and multifactorial. Although global awareness regarding HIV-1/HCV co-infection is increasing little is known about the pathophysiology that mediates th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
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| Series: | Clinical Proteomics |
| Subjects: | |
| Online Access: | http://www.clinicalproteomicsjournal.com/content/9/1/11 |
| _version_ | 1818486804900216832 |
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| author | Boukli Nawal M Shetty Vivekananda Cubano Luis Ricaurte Martha Coelho-dos-Reis Jordana Nickens Zacharie Shah Punit Talal Andrew H Philip Ramila Jain Pooja |
| author_facet | Boukli Nawal M Shetty Vivekananda Cubano Luis Ricaurte Martha Coelho-dos-Reis Jordana Nickens Zacharie Shah Punit Talal Andrew H Philip Ramila Jain Pooja |
| author_sort | Boukli Nawal M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Pathogenesis of liver damage in patients with HIV and HCV co-infection is complex and multifactorial. Although global awareness regarding HIV-1/HCV co-infection is increasing little is known about the pathophysiology that mediates the rapid progression to hepatic disease in the co-infected individuals.</p> <p>Results</p> <p>In this study, we investigated the proteome profiles of peripheral blood mononuclear cells from HIV-1 mono-, HCV mono-, and HIV-1/HCV co-infected patients. The results of high-resolution 2D gel electrophoresis and PD quest software quantitative analysis revealed that several proteins were differentially expressed in HIV-1, HCV, and HIV-1/HCV co-infection. Liquid chromatography-mass spectrometry and Mascot database matching (LC-MS/MS analysis) successfully identified 29 unique and differentially expressed proteins. These included cytoskeletal proteins (tropomyosin, gelsolin, DYPLSL3, DYPLSL4 and profilin-1), chaperones and co-chaperones (HSP90-beta and stress-induced phosphoprotein), metabolic and pre-apoptotic proteins (guanosine triphosphate [GTP]-binding nuclear protein Ran, the detoxifying enzyme glutathione S-transferase (GST) and Rho GDP-dissociation inhibitor (Rho-GDI), proteins involved in cell prosurvival mechanism, and those involved in matrix synthesis (collagen binding protein 2 [CBP2]). The six most significant and relevant proteins were further validated in a group of mono- and co-infected patients (n = 20) at the transcriptional levels.</p> <p>Conclusions</p> <p>The specific pro- and anti- apoptotic protein signatures revealed in this study could facilitate the understanding of apoptotic and protective immune-mediated mechanisms underlying HIV-1 and HCV co-infection and their implications on liver disease progression in co-infected patients.</p> |
| first_indexed | 2024-12-10T16:27:54Z |
| format | Article |
| id | doaj.art-5a9f5af66a5b4395b2b201a363701fa7 |
| institution | Directory Open Access Journal |
| issn | 1559-0275 |
| language | English |
| last_indexed | 2024-12-10T16:27:54Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Proteomics |
| spelling | doaj.art-5a9f5af66a5b4395b2b201a363701fa72022-12-22T01:41:36ZengBMCClinical Proteomics1559-02752012-09-01911110.1186/1559-0275-9-11Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patientsBoukli Nawal MShetty VivekanandaCubano LuisRicaurte MarthaCoelho-dos-Reis JordanaNickens ZacharieShah PunitTalal Andrew HPhilip RamilaJain Pooja<p>Abstract</p> <p>Background</p> <p>Pathogenesis of liver damage in patients with HIV and HCV co-infection is complex and multifactorial. Although global awareness regarding HIV-1/HCV co-infection is increasing little is known about the pathophysiology that mediates the rapid progression to hepatic disease in the co-infected individuals.</p> <p>Results</p> <p>In this study, we investigated the proteome profiles of peripheral blood mononuclear cells from HIV-1 mono-, HCV mono-, and HIV-1/HCV co-infected patients. The results of high-resolution 2D gel electrophoresis and PD quest software quantitative analysis revealed that several proteins were differentially expressed in HIV-1, HCV, and HIV-1/HCV co-infection. Liquid chromatography-mass spectrometry and Mascot database matching (LC-MS/MS analysis) successfully identified 29 unique and differentially expressed proteins. These included cytoskeletal proteins (tropomyosin, gelsolin, DYPLSL3, DYPLSL4 and profilin-1), chaperones and co-chaperones (HSP90-beta and stress-induced phosphoprotein), metabolic and pre-apoptotic proteins (guanosine triphosphate [GTP]-binding nuclear protein Ran, the detoxifying enzyme glutathione S-transferase (GST) and Rho GDP-dissociation inhibitor (Rho-GDI), proteins involved in cell prosurvival mechanism, and those involved in matrix synthesis (collagen binding protein 2 [CBP2]). The six most significant and relevant proteins were further validated in a group of mono- and co-infected patients (n = 20) at the transcriptional levels.</p> <p>Conclusions</p> <p>The specific pro- and anti- apoptotic protein signatures revealed in this study could facilitate the understanding of apoptotic and protective immune-mediated mechanisms underlying HIV-1 and HCV co-infection and their implications on liver disease progression in co-infected patients.</p>http://www.clinicalproteomicsjournal.com/content/9/1/11HIV-1HCVHIV-1/HCV2D-GEMass spectrometryPro- and anti-apoptotic fingerprintingProteomics |
| spellingShingle | Boukli Nawal M Shetty Vivekananda Cubano Luis Ricaurte Martha Coelho-dos-Reis Jordana Nickens Zacharie Shah Punit Talal Andrew H Philip Ramila Jain Pooja Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients Clinical Proteomics HIV-1 HCV HIV-1/HCV 2D-GE Mass spectrometry Pro- and anti-apoptotic fingerprinting Proteomics |
| title | Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients |
| title_full | Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients |
| title_fullStr | Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients |
| title_full_unstemmed | Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients |
| title_short | Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients |
| title_sort | unique and differential protein signatures within the mononuclear cells of hiv 1 and hcv mono infected and co infected patients |
| topic | HIV-1 HCV HIV-1/HCV 2D-GE Mass spectrometry Pro- and anti-apoptotic fingerprinting Proteomics |
| url | http://www.clinicalproteomicsjournal.com/content/9/1/11 |
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