New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides
NK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig’s small intestine. In previous work, for the first time, we identifie...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1191966/full |
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| author | Leonardo Ortega Crisleri Carrera Carolina Muñoz-Flores Santiago Salazar Milton F. Villegas María F. Starck Ariel Valenzuela Niza Agurto Raquel Montesino Allisson Astuya Natalie Parra Ercilia T. Pérez Ercilia T. Pérez Natacha Santibáñez Natacha Santibáñez Alex Romero Alex Romero Pamela Ruíz Emilio Lamazares Fátima Reyes Oliberto Sánchez Jorge R. Toledo Jannel Acosta |
| author_facet | Leonardo Ortega Crisleri Carrera Carolina Muñoz-Flores Santiago Salazar Milton F. Villegas María F. Starck Ariel Valenzuela Niza Agurto Raquel Montesino Allisson Astuya Natalie Parra Ercilia T. Pérez Ercilia T. Pérez Natacha Santibáñez Natacha Santibáñez Alex Romero Alex Romero Pamela Ruíz Emilio Lamazares Fátima Reyes Oliberto Sánchez Jorge R. Toledo Jannel Acosta |
| author_sort | Leonardo Ortega |
| collection | DOAJ |
| description | NK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig’s small intestine. In previous work, for the first time, we identified four variants of nk-lysin from Atlantic salmon (Salmo salar) using EST sequences. In the present study, we reported and characterized two additional transcripts of NK-lysin from S. salar. Besides, we evaluated the tissue distribution of three NK-lysins from S. salar and assessed the antimicrobial, hemolytic, and immunomodulatory activities and signaling pathways of three NK-lysin-derived peptides. The synthetic peptides displayed antimicrobial activity against Piscirickettsia salmonis (LF-89) and Flavobacterium psychrophilum. These peptides induced the expression of immune genes related to innate and adaptive immune responses in vitro and in vivo. The immunomodulatory activity of the peptides involves the mitogen-activated protein kinases-mediated signaling pathway, including p38, extracellular signal-regulated kinase 1/2, and/or c-Jun N-terminal kinases. Besides, the peptides modulated the immune response induced by pathogen-associated molecular patterns (PAMPs). Our findings show that NK-lysin could be a highly effective immunostimulant or vaccine adjuvant for use in fish aquaculture. |
| first_indexed | 2024-04-24T11:55:25Z |
| format | Article |
| id | doaj.art-5a9fe38e9a21442d9d60b7bc9ce3877a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-24T11:55:25Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-5a9fe38e9a21442d9d60b7bc9ce3877a2024-04-09T04:46:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-04-011510.3389/fimmu.2024.11919661191966New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptidesLeonardo Ortega0Crisleri Carrera1Carolina Muñoz-Flores2Santiago Salazar3Milton F. Villegas4María F. Starck5Ariel Valenzuela6Niza Agurto7Raquel Montesino8Allisson Astuya9Natalie Parra10Ercilia T. Pérez11Ercilia T. Pérez12Natacha Santibáñez13Natacha Santibáñez14Alex Romero15Alex Romero16Pamela Ruíz17Emilio Lamazares18Fátima Reyes19Oliberto Sánchez20Jorge R. Toledo21Jannel Acosta22Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Piscicultura y Patología Acuática, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, ChileLaboratorio de Piscicultura y Patología Acuática, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Genómica Marina y Cultivo Celular, Departamento de Oceanografía y Centro de Investigación Oceanográfica en el Pacífico Sur Oriental (COPAS) Sur-Austral, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Inmunología y Estrés de Organismos Acuáticos, Instituto de Patología Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, ChileCentro Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP), Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, Concepción, ChileLaboratorio de Inmunología y Estrés de Organismos Acuáticos, Instituto de Patología Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, ChileCentro Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP), Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, Concepción, ChileLaboratorio de Inmunología y Estrés de Organismos Acuáticos, Instituto de Patología Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, ChileCentro Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP), Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, Concepción, ChileDepartamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Talcahuano, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biofármacos Recombinantes, Departamento de Farmacología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biofármacos Recombinantes, Departamento de Farmacología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileNK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig’s small intestine. In previous work, for the first time, we identified four variants of nk-lysin from Atlantic salmon (Salmo salar) using EST sequences. In the present study, we reported and characterized two additional transcripts of NK-lysin from S. salar. Besides, we evaluated the tissue distribution of three NK-lysins from S. salar and assessed the antimicrobial, hemolytic, and immunomodulatory activities and signaling pathways of three NK-lysin-derived peptides. The synthetic peptides displayed antimicrobial activity against Piscirickettsia salmonis (LF-89) and Flavobacterium psychrophilum. These peptides induced the expression of immune genes related to innate and adaptive immune responses in vitro and in vivo. The immunomodulatory activity of the peptides involves the mitogen-activated protein kinases-mediated signaling pathway, including p38, extracellular signal-regulated kinase 1/2, and/or c-Jun N-terminal kinases. Besides, the peptides modulated the immune response induced by pathogen-associated molecular patterns (PAMPs). Our findings show that NK-lysin could be a highly effective immunostimulant or vaccine adjuvant for use in fish aquaculture.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1191966/fullNK-lysinantimicrobial peptideimmune responsecytokinesSalmo salar |
| spellingShingle | Leonardo Ortega Crisleri Carrera Carolina Muñoz-Flores Santiago Salazar Milton F. Villegas María F. Starck Ariel Valenzuela Niza Agurto Raquel Montesino Allisson Astuya Natalie Parra Ercilia T. Pérez Ercilia T. Pérez Natacha Santibáñez Natacha Santibáñez Alex Romero Alex Romero Pamela Ruíz Emilio Lamazares Fátima Reyes Oliberto Sánchez Jorge R. Toledo Jannel Acosta New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides Frontiers in Immunology NK-lysin antimicrobial peptide immune response cytokines Salmo salar |
| title | New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides |
| title_full | New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides |
| title_fullStr | New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides |
| title_full_unstemmed | New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides |
| title_short | New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides |
| title_sort | new insight into the biological activity of salmo salar nk lysin antimicrobial peptides |
| topic | NK-lysin antimicrobial peptide immune response cytokines Salmo salar |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1191966/full |
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