Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk”
Microsporia is a common infectious disease in the practice of a veterinarian. This disease carries a risk of human infection because it is anthropozoonotic. The main pathogen Microsporum sanis affects the upper layers of the skin and has the ability to stay on the body of an animal for up to two yea...
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| Format: | Article |
| Language: | English |
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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
2021-03-01
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| Series: | Ukrainian Journal of Veterinary and Agricultural Sciences |
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| Online Access: | https://ujvas.com.ua/index.php/journal/article/view/78 |
| _version_ | 1818655727478112256 |
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| author | Y. V. Martyniv Ya. V. Kisera |
| author_facet | Y. V. Martyniv Ya. V. Kisera |
| author_sort | Y. V. Martyniv |
| collection | DOAJ |
| description | Microsporia is a common infectious disease in the practice of a veterinarian. This disease carries a risk of human infection because it is anthropozoonotic. The main pathogen Microsporum sanis affects the upper layers of the skin and has the ability to stay on the body of an animal for up to two years. Therefore, treatment should be carried out by a complex method and be aimed at preventing the spread of the pathogen in the environment and increase the body's resistance in the fight against the disease. “Micromar” based on clotrimazole and povidone iodine and the immunostimulant “Biogluc” based on beta glucan and biotin were used to treat patients with microsporia. Clotrimazole and povidone iodine have antifungal and antiseptic properties, and beta glucan in combination with biotin stimulates the immune response and accelerates the regeneration of damaged skin. In order to determine the immune reactivity of the organism in the treatment of microsporia, hematological, immunological blood tests and histological examinations of the skin of guinea pigs infected with the pathogen M. canis were performed. The obtained results showed that when using the antifungal drug “Micromar” and immunostimulant “Biogluk” the number of leukocytes decreases from 11.13 ± 0.72 to 6.95 ± 0.10 109/L, rod-shaped neutrophils from 16.00 ± 1.29 to 6.17 ± 0.65 %, ESR from 5.67 ± 0.67 to 2.17 ± 0.31 mm/h, and the number of segmental neutrophils increases from 12.17 ± 1.47 to 22.00 ± 0, 86 %. There is a tendency to increase T-helpers. These changes indicate the development of an active immune response to the inflammatory process. In the structure of the skin there is a decrease in exudation and infiltration in the dermis, active trophism of hair follicles, which indicates the course of regenerative processes. |
| first_indexed | 2024-12-17T03:14:17Z |
| format | Article |
| id | doaj.art-5aa75defd2d94f9b8d85b9a7c84bd348 |
| institution | Directory Open Access Journal |
| issn | 2617-6149 |
| language | English |
| last_indexed | 2024-12-17T03:14:17Z |
| publishDate | 2021-03-01 |
| publisher | Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv |
| record_format | Article |
| series | Ukrainian Journal of Veterinary and Agricultural Sciences |
| spelling | doaj.art-5aa75defd2d94f9b8d85b9a7c84bd3482022-12-21T22:05:43ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivUkrainian Journal of Veterinary and Agricultural Sciences2617-61492021-03-0141293210.32718/ujvas4-1.0678Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk”Y. V. Martyniv0Ya. V. Kisera1Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, UkraineStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Pekarska Str., 50, Lviv, 79010, UkraineMicrosporia is a common infectious disease in the practice of a veterinarian. This disease carries a risk of human infection because it is anthropozoonotic. The main pathogen Microsporum sanis affects the upper layers of the skin and has the ability to stay on the body of an animal for up to two years. Therefore, treatment should be carried out by a complex method and be aimed at preventing the spread of the pathogen in the environment and increase the body's resistance in the fight against the disease. “Micromar” based on clotrimazole and povidone iodine and the immunostimulant “Biogluc” based on beta glucan and biotin were used to treat patients with microsporia. Clotrimazole and povidone iodine have antifungal and antiseptic properties, and beta glucan in combination with biotin stimulates the immune response and accelerates the regeneration of damaged skin. In order to determine the immune reactivity of the organism in the treatment of microsporia, hematological, immunological blood tests and histological examinations of the skin of guinea pigs infected with the pathogen M. canis were performed. The obtained results showed that when using the antifungal drug “Micromar” and immunostimulant “Biogluk” the number of leukocytes decreases from 11.13 ± 0.72 to 6.95 ± 0.10 109/L, rod-shaped neutrophils from 16.00 ± 1.29 to 6.17 ± 0.65 %, ESR from 5.67 ± 0.67 to 2.17 ± 0.31 mm/h, and the number of segmental neutrophils increases from 12.17 ± 1.47 to 22.00 ± 0, 86 %. There is a tendency to increase T-helpers. These changes indicate the development of an active immune response to the inflammatory process. In the structure of the skin there is a decrease in exudation and infiltration in the dermis, active trophism of hair follicles, which indicates the course of regenerative processes.https://ujvas.com.ua/index.php/journal/article/view/78guinea pig, microsporia, clotrimazole, povidone iodine, beta glucan, blood, leukocytes, lymphocytes, neutro-phils. epidermis, dermis |
| spellingShingle | Y. V. Martyniv Ya. V. Kisera Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” Ukrainian Journal of Veterinary and Agricultural Sciences guinea pig, microsporia, clotrimazole, povidone iodine, beta glucan, blood, leukocytes, lymphocytes, neutro-phils. epidermis, dermis |
| title | Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” |
| title_full | Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” |
| title_fullStr | Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” |
| title_full_unstemmed | Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” |
| title_short | Hematological, immunological and histological changes in guinea pigs in the treatment of microsporia with drugs “Micromar” and “Biogluk” |
| title_sort | hematological immunological and histological changes in guinea pigs in the treatment of microsporia with drugs micromar and biogluk |
| topic | guinea pig, microsporia, clotrimazole, povidone iodine, beta glucan, blood, leukocytes, lymphocytes, neutro-phils. epidermis, dermis |
| url | https://ujvas.com.ua/index.php/journal/article/view/78 |
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