Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa
The epidemiology of corresponding tick-borne diseases has changed as a result of the recent introduction of <i>Rhipicephalus (Boophilus) microplus</i> to West Africa. The current study aimed to assess the diagnostic performance of an indirect ELISA for the detection of <i>Babesia b...
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MDPI AG
2023-01-01
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| author | Alassane Toure Moussa Sanogo Abdelmalek Sghiri Hamid Sahibi |
| author_facet | Alassane Toure Moussa Sanogo Abdelmalek Sghiri Hamid Sahibi |
| author_sort | Alassane Toure |
| collection | DOAJ |
| description | The epidemiology of corresponding tick-borne diseases has changed as a result of the recent introduction of <i>Rhipicephalus (Boophilus) microplus</i> to West Africa. The current study aimed to assess the diagnostic performance of an indirect ELISA for the detection of <i>Babesia bovis</i> infection in cattle. In a cross-section study, using a Bayesian Latent Class Model and iELISA diagnostic test for cattle babesiosis due to <i>Babesia bovis</i>, accuracy has been assessed with RT-PCR as an imperfect reference test. A total of 766 cattle were tested. The optimal diagnostic performances were obtained with 5% percentage of positivity. Sensitivity and specificity were, respectively, 0.94 [Cr. I.: 0.85–0.99] and 0.89 [Cr. I.: 0.87–0.92]. Additional diagnostic characteristics revealed that the Positive Predictive Value (PPV) and Negative Predictive Value (NPV) were 96.6% [Cr. I.: 92.7–100%] and 82.2% [Cr. I.: 72–93%]. Overall, this test well discriminates an infected status from an uninfected status considering the area under the ROC curve (AUC) which was 0.78 [Cr. I: 0.72–0.85] and a Diagnostic Odds Ratio (DOR) of 127.8 [Cr. I.: 10.43–1562.27]. The AUC was significantly higher than 0.5 (<i>p</i> < 10<sup>−5</sup>). In consequence, this serologic assay could be suitable in moderate to high prevalence assessments. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T11:55:31Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-5ab742160e7a4aa78c7d8ac53fed41d62023-11-30T23:09:26ZengMDPI AGLife2075-17292023-01-0113120310.3390/life13010203Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West AfricaAlassane Toure0Moussa Sanogo1Abdelmalek Sghiri2Hamid Sahibi3UFR Sciences de la Nature, Nangui Abrogoua University, P.O. Box 801 Abidjan 02, Côte d’IvoireLaboratoire Central Vétérinaire de Bingerville, LANADA, P.O. Box 206 Bingerville, Côte d’IvoireInstitut Agronomique et Vétérinaire Hassan II, Madinat Al Irfane, P.O. Box 6202, Rabat 10101, MoroccoInstitut Agronomique et Vétérinaire Hassan II, Madinat Al Irfane, P.O. Box 6202, Rabat 10101, MoroccoThe epidemiology of corresponding tick-borne diseases has changed as a result of the recent introduction of <i>Rhipicephalus (Boophilus) microplus</i> to West Africa. The current study aimed to assess the diagnostic performance of an indirect ELISA for the detection of <i>Babesia bovis</i> infection in cattle. In a cross-section study, using a Bayesian Latent Class Model and iELISA diagnostic test for cattle babesiosis due to <i>Babesia bovis</i>, accuracy has been assessed with RT-PCR as an imperfect reference test. A total of 766 cattle were tested. The optimal diagnostic performances were obtained with 5% percentage of positivity. Sensitivity and specificity were, respectively, 0.94 [Cr. I.: 0.85–0.99] and 0.89 [Cr. I.: 0.87–0.92]. Additional diagnostic characteristics revealed that the Positive Predictive Value (PPV) and Negative Predictive Value (NPV) were 96.6% [Cr. I.: 92.7–100%] and 82.2% [Cr. I.: 72–93%]. Overall, this test well discriminates an infected status from an uninfected status considering the area under the ROC curve (AUC) which was 0.78 [Cr. I: 0.72–0.85] and a Diagnostic Odds Ratio (DOR) of 127.8 [Cr. I.: 10.43–1562.27]. The AUC was significantly higher than 0.5 (<i>p</i> < 10<sup>−5</sup>). In consequence, this serologic assay could be suitable in moderate to high prevalence assessments.https://www.mdpi.com/2075-1729/13/1/203<i>Babesia bovis</i>cattleiELISAindex testBayesianRT-PCR |
| spellingShingle | Alassane Toure Moussa Sanogo Abdelmalek Sghiri Hamid Sahibi Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa Life <i>Babesia bovis</i> cattle iELISA index test Bayesian RT-PCR |
| title | Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa |
| title_full | Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa |
| title_fullStr | Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa |
| title_full_unstemmed | Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa |
| title_short | Diagnostic Accuracy of an Indirect Enzyme Linked Immunosorbent Assay (iELISA) for Screening of <i>Babesia bovis</i> in Cattle from West Africa |
| title_sort | diagnostic accuracy of an indirect enzyme linked immunosorbent assay ielisa for screening of i babesia bovis i in cattle from west africa |
| topic | <i>Babesia bovis</i> cattle iELISA index test Bayesian RT-PCR |
| url | https://www.mdpi.com/2075-1729/13/1/203 |
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