First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea
Abstract Background Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts of the world. At least 15 distinct tick species belonging to six genera have shown the presence of Rickettsia...
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BMC
2021-04-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-021-04695-5 |
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| author | Misbah Tariq Jun-Won Seo Da Young Kim Merlin Jayalal Lawrence Panchali Na Ra Yun You Mi Lee Choon-Mee Kim Dong-Min Kim |
| author_facet | Misbah Tariq Jun-Won Seo Da Young Kim Merlin Jayalal Lawrence Panchali Na Ra Yun You Mi Lee Choon-Mee Kim Dong-Min Kim |
| author_sort | Misbah Tariq |
| collection | DOAJ |
| description | Abstract Background Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts of the world. At least 15 distinct tick species belonging to six genera have shown the presence of Rickettsia raoultii. Herein, we report the detection of R. raoultii in ticks from the Republic of Korea (ROK). Methods Thirty-five ticks were collected from 29 patients with tick bites in Gwangju Metropolitan City, Jeollanam Province, ROK. The ticks were identified using molecular, morphological, and taxonomic characteristics. All samples were screened for presence of Rickettsia species using nested polymerase chain reactions of their outer membrane protein (ompA) and citrate synthase (gltA) genes. The amplified products were sequenced for subsequent phylogenetic analyses. Results Sequencing data showed the DNA sequences of R. raoultii in three Haemaphysalis longicornis ticks. All three tick samples were 99.4–100% similar to previously reported partial sequences of ompA of R. raoultii strains CP019435 and MF002523, which formed a single clade with the reference strains. Conclusions We provide the first description and molecular identification of R. raoultii detected in H. longicornis ticks in the ROK. This observation extends the geographical distribution of R. raoultii. Screening of human samples for this pathogen will provide information about the prevalence of rickettsial infections in this region. |
| first_indexed | 2024-12-14T15:24:12Z |
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| id | doaj.art-5ac0bf1828d342efa34dbb03abe5146c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-14T15:24:12Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-5ac0bf1828d342efa34dbb03abe5146c2022-12-21T22:56:03ZengBMCParasites & Vectors1756-33052021-04-011411510.1186/s13071-021-04695-5First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of KoreaMisbah Tariq0Jun-Won Seo1Da Young Kim2Merlin Jayalal Lawrence Panchali3Na Ra Yun4You Mi Lee5Choon-Mee Kim6Dong-Min Kim7Department of Internal Medicine, College of Medicine, Chosun UniversityDepartment of Internal Medicine, College of Medicine, Chosun UniversityDepartment of Internal Medicine, College of Medicine, Chosun UniversityDepartment of Premedical Science, College of Medicine, Chosun UniversityDepartment of Internal Medicine, College of Medicine, Chosun UniversityDepartment of Internal Medicine, College of Medicine, Chosun UniversityDepartment of Premedical Science, College of Medicine, Chosun UniversityDepartment of Internal Medicine, College of Medicine, Chosun UniversityAbstract Background Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts of the world. At least 15 distinct tick species belonging to six genera have shown the presence of Rickettsia raoultii. Herein, we report the detection of R. raoultii in ticks from the Republic of Korea (ROK). Methods Thirty-five ticks were collected from 29 patients with tick bites in Gwangju Metropolitan City, Jeollanam Province, ROK. The ticks were identified using molecular, morphological, and taxonomic characteristics. All samples were screened for presence of Rickettsia species using nested polymerase chain reactions of their outer membrane protein (ompA) and citrate synthase (gltA) genes. The amplified products were sequenced for subsequent phylogenetic analyses. Results Sequencing data showed the DNA sequences of R. raoultii in three Haemaphysalis longicornis ticks. All three tick samples were 99.4–100% similar to previously reported partial sequences of ompA of R. raoultii strains CP019435 and MF002523, which formed a single clade with the reference strains. Conclusions We provide the first description and molecular identification of R. raoultii detected in H. longicornis ticks in the ROK. This observation extends the geographical distribution of R. raoultii. Screening of human samples for this pathogen will provide information about the prevalence of rickettsial infections in this region.https://doi.org/10.1186/s13071-021-04695-5Rickettsia raoultiiTicksHaemaphysalis longicornisSpotted fever group |
| spellingShingle | Misbah Tariq Jun-Won Seo Da Young Kim Merlin Jayalal Lawrence Panchali Na Ra Yun You Mi Lee Choon-Mee Kim Dong-Min Kim First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea Parasites & Vectors Rickettsia raoultii Ticks Haemaphysalis longicornis Spotted fever group |
| title | First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea |
| title_full | First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea |
| title_fullStr | First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea |
| title_full_unstemmed | First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea |
| title_short | First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea |
| title_sort | first report of the molecular detection of human pathogen rickettsia raoultii in ticks from the republic of korea |
| topic | Rickettsia raoultii Ticks Haemaphysalis longicornis Spotted fever group |
| url | https://doi.org/10.1186/s13071-021-04695-5 |
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