An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution
Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/60005 |
| _version_ | 1797997556632911872 |
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| author | Aubrey GA Howard IV Phillip A Baker Rodrigo Ibarra-García-Padilla Joshua A Moore Lucia J Rivas James J Tallman Eileen W Singleton Jessa L Westheimer Julia A Corteguera Rosa A Uribe |
| author_facet | Aubrey GA Howard IV Phillip A Baker Rodrigo Ibarra-García-Padilla Joshua A Moore Lucia J Rivas James J Tallman Eileen W Singleton Jessa L Westheimer Julia A Corteguera Rosa A Uribe |
| author_sort | Aubrey GA Howard IV |
| collection | DOAJ |
| description | Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC-derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme cells, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation. |
| first_indexed | 2024-04-11T10:34:31Z |
| format | Article |
| id | doaj.art-5ac3089d892c4ce1ad2469258e979166 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:31Z |
| publishDate | 2021-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5ac3089d892c4ce1ad2469258e9791662022-12-22T04:29:19ZengeLife Sciences Publications LtdeLife2050-084X2021-02-011010.7554/eLife.60005An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolutionAubrey GA Howard IV0Phillip A Baker1Rodrigo Ibarra-García-Padilla2Joshua A Moore3Lucia J Rivas4James J Tallman5Eileen W Singleton6Jessa L Westheimer7Julia A Corteguera8Rosa A Uribe9https://orcid.org/0000-0002-0427-4493Department of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesDepartment of BioSciences, Rice University, Houston, United StatesNeural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC-derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme cells, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation.https://elifesciences.org/articles/60005neural crestmelanocyteentericneural progenitortranscriptomesox10 |
| spellingShingle | Aubrey GA Howard IV Phillip A Baker Rodrigo Ibarra-García-Padilla Joshua A Moore Lucia J Rivas James J Tallman Eileen W Singleton Jessa L Westheimer Julia A Corteguera Rosa A Uribe An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution eLife neural crest melanocyte enteric neural progenitor transcriptome sox10 |
| title | An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution |
| title_full | An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution |
| title_fullStr | An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution |
| title_full_unstemmed | An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution |
| title_short | An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution |
| title_sort | atlas of neural crest lineages along the posterior developing zebrafish at single cell resolution |
| topic | neural crest melanocyte enteric neural progenitor transcriptome sox10 |
| url | https://elifesciences.org/articles/60005 |
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