<b>Determination of serum albumin with tribromoarsenazo by spectrophotometry</b>
The reaction of tribromoarsenazo(TB-ASA) with serum albumin in the presence of emulgent OP was studied by spectrophotometry. In a Britton-Robinson buffer solution at pH 2.9, tribromoarsenazo and bovine serum albumin can immediately form a red compound in the presence of emulgent OP with a maximum ab...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Chemical Society of Ethiopia
2007-08-01
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| Series: | Bulletin of the Chemical Society of Ethiopia |
| Subjects: | |
| Online Access: | http://www.ajol.info/index.php/bcse/article/view/21209 |
| Summary: | The reaction of tribromoarsenazo(TB-ASA) with serum albumin in the presence of emulgent OP was studied by spectrophotometry. In a Britton-Robinson buffer solution at pH 2.9, tribromoarsenazo and bovine serum albumin can immediately form a red compound in the presence of emulgent OP with a maximum absorption wavelength at 354 nm. The presence of emulgent OP can increase the reaction sensitivity and the compound stability. The molar absorptivity of the compound is ε<sub>354 nm</sub> = 6.13 x 105 M<sup>-1</sup>•cm<sup>-1</sup>. Beer's law is obeyed over the range of 5.0-75.0 mg•L<sup>-1</sup> for bovine serum albumin. The present method was applied to the determination of the total proteins in human serums with satisfactory results. |
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| ISSN: | 1011-3924 1726-801X |