Methods for the determination of antioxidant activity of plant extracts in vitro

Plants are rich sources of secondary metabolites that exhibit diverse biological and pharmacological effects. Some plant ingredients, primarily phenolics, have significant in vitro antioxidant activity, which implies their contribution to the maintenance of redox balance in the body. These potential...

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Main Author: Kukić-Marković Jelena
Format: Article
Language:srp
Published: Pharmaceutical Association of Serbia, Belgrade, Serbia 2023-01-01
Series:Arhiv za farmaciju
Subjects:
Online Access:https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2023/0004-19632304318K.pdf
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author Kukić-Marković Jelena
author_facet Kukić-Marković Jelena
author_sort Kukić-Marković Jelena
collection DOAJ
description Plants are rich sources of secondary metabolites that exhibit diverse biological and pharmacological effects. Some plant ingredients, primarily phenolics, have significant in vitro antioxidant activity, which implies their contribution to the maintenance of redox balance in the body. These potential antioxidant agents are structurally very diverse, having different mechanisms of antioxidant activity. Since there is a growing necessity to detect, develop and understand effective antioxidant compounds, interest in the identification and the measurement of antioxidants in various plant isolates is persistently growing and many methods are being established. Most of the available in vitro tests are affordable and easy to perform, but due to the complex composition of plant extracts, different kinetics, mechanisms and specificity of the chemical reactions underlying these tests, there is no universal parameter for the assessment of antioxidant activity. In this paper, some of the currently most used in vitro methods for investigating and evaluating antioxidant activity of plant extracts are presented, emphasizing their advantages and weaknesses.
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spelling doaj.art-5ac486f29b6e4e9d9a685c04601818682023-10-04T08:23:52ZsrpPharmaceutical Association of Serbia, Belgrade, SerbiaArhiv za farmaciju0004-19632217-87672023-01-0173431833510.5937/arhfarm73-385730004-19632304318KMethods for the determination of antioxidant activity of plant extracts in vitroKukić-Marković Jelena0https://orcid.org/0000-0002-3326-0728University of Belgrade, Faculty of Pharmacy, Department of Pharmacognosy, Belgrade, SerbiaPlants are rich sources of secondary metabolites that exhibit diverse biological and pharmacological effects. Some plant ingredients, primarily phenolics, have significant in vitro antioxidant activity, which implies their contribution to the maintenance of redox balance in the body. These potential antioxidant agents are structurally very diverse, having different mechanisms of antioxidant activity. Since there is a growing necessity to detect, develop and understand effective antioxidant compounds, interest in the identification and the measurement of antioxidants in various plant isolates is persistently growing and many methods are being established. Most of the available in vitro tests are affordable and easy to perform, but due to the complex composition of plant extracts, different kinetics, mechanisms and specificity of the chemical reactions underlying these tests, there is no universal parameter for the assessment of antioxidant activity. In this paper, some of the currently most used in vitro methods for investigating and evaluating antioxidant activity of plant extracts are presented, emphasizing their advantages and weaknesses.https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2023/0004-19632304318K.pdfantioxidantsplant extractsin vitro tests
spellingShingle Kukić-Marković Jelena
Methods for the determination of antioxidant activity of plant extracts in vitro
Arhiv za farmaciju
antioxidants
plant extracts
in vitro tests
title Methods for the determination of antioxidant activity of plant extracts in vitro
title_full Methods for the determination of antioxidant activity of plant extracts in vitro
title_fullStr Methods for the determination of antioxidant activity of plant extracts in vitro
title_full_unstemmed Methods for the determination of antioxidant activity of plant extracts in vitro
title_short Methods for the determination of antioxidant activity of plant extracts in vitro
title_sort methods for the determination of antioxidant activity of plant extracts in vitro
topic antioxidants
plant extracts
in vitro tests
url https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2023/0004-19632304318K.pdf
work_keys_str_mv AT kukicmarkovicjelena methodsforthedeterminationofantioxidantactivityofplantextractsinvitro