Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium
For decades, the social amoeba Dictyostelium discoideum has been an invaluable tool for dissecting the biology of eukaryotic cells. Its short growth cycle and genetic tractability make it ideal for a variety of biochemical, cell biological, and biophysical assays. Dictyostelium have been widely used...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.740205/full |
| _version_ | 1819127849504735232 |
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| author | Jonathan Kuhn Yiyan Lin Peter N. Devreotes |
| author_facet | Jonathan Kuhn Yiyan Lin Peter N. Devreotes |
| author_sort | Jonathan Kuhn |
| collection | DOAJ |
| description | For decades, the social amoeba Dictyostelium discoideum has been an invaluable tool for dissecting the biology of eukaryotic cells. Its short growth cycle and genetic tractability make it ideal for a variety of biochemical, cell biological, and biophysical assays. Dictyostelium have been widely used as a model of eukaryotic cell motility because the signaling and mechanical networks which they use to steer and produce forward motion are highly conserved. Because these migration networks consist of hundreds of interconnected proteins, perturbing individual molecules can have subtle effects or alter cell morphology and signaling in major unpredictable ways. Therefore, to fully understand this network, we must be able to quantitatively assess the consequences of abrupt modifications. This ability will allow us better control cell migration, which is critical for development and disease, in vivo. Here, we review recent advances in imaging, synthetic biology, and computational analysis which enable researchers to tune the activity of individual molecules in single living cells and precisely measure the effects on cellular motility and signaling. We also provide practical advice and resources to assist in applying these approaches in Dictyostelium. |
| first_indexed | 2024-12-22T08:18:28Z |
| format | Article |
| id | doaj.art-5ac6a95de58541b8a0d806211b3e9966 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-22T08:18:28Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-5ac6a95de58541b8a0d806211b3e99662022-12-21T18:32:49ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.740205740205Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in DictyosteliumJonathan KuhnYiyan LinPeter N. DevreotesFor decades, the social amoeba Dictyostelium discoideum has been an invaluable tool for dissecting the biology of eukaryotic cells. Its short growth cycle and genetic tractability make it ideal for a variety of biochemical, cell biological, and biophysical assays. Dictyostelium have been widely used as a model of eukaryotic cell motility because the signaling and mechanical networks which they use to steer and produce forward motion are highly conserved. Because these migration networks consist of hundreds of interconnected proteins, perturbing individual molecules can have subtle effects or alter cell morphology and signaling in major unpredictable ways. Therefore, to fully understand this network, we must be able to quantitatively assess the consequences of abrupt modifications. This ability will allow us better control cell migration, which is critical for development and disease, in vivo. Here, we review recent advances in imaging, synthetic biology, and computational analysis which enable researchers to tune the activity of individual molecules in single living cells and precisely measure the effects on cellular motility and signaling. We also provide practical advice and resources to assist in applying these approaches in Dictyostelium.https://www.frontiersin.org/articles/10.3389/fcell.2021.740205/fullmigrationchemotaxissignalingimagingsynthetic & systems biology |
| spellingShingle | Jonathan Kuhn Yiyan Lin Peter N. Devreotes Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium Frontiers in Cell and Developmental Biology migration chemotaxis signaling imaging synthetic & systems biology |
| title | Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium |
| title_full | Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium |
| title_fullStr | Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium |
| title_full_unstemmed | Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium |
| title_short | Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium |
| title_sort | using live cell imaging and synthetic biology to probe directed migration in dictyostelium |
| topic | migration chemotaxis signaling imaging synthetic & systems biology |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.740205/full |
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