Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
Abstract The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the au...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-10-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202302072 |
| _version_ | 1797663867993587712 |
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| author | Kiran Shrestha Seongryeong Kim Jiyeon Han Gabriela Morales Florez Han Truong Trung Hoang Sajjan Parajuli Tiara AM Beomsoo Kim Younsu Jung Abdurhaman Teyib Abafogi Yugyeong Lee Seung Hyun Song Jinkee Lee Sungsu Park Minhee Kang Hee Jae Huh Gyoujin Cho Luke P. Lee |
| author_facet | Kiran Shrestha Seongryeong Kim Jiyeon Han Gabriela Morales Florez Han Truong Trung Hoang Sajjan Parajuli Tiara AM Beomsoo Kim Younsu Jung Abdurhaman Teyib Abafogi Yugyeong Lee Seung Hyun Song Jinkee Lee Sungsu Park Minhee Kang Hee Jae Huh Gyoujin Cho Luke P. Lee |
| author_sort | Kiran Shrestha |
| collection | DOAJ |
| description | Abstract The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on‐chip ‐RT‐quantitative PCR (RT‐qPCR): MEDIC‐PCR. First, the authors use a roll‐to‐roll printing process to accomplish low‐cost carbon‐black‐based disposable PCR chips that enable rapid LED‐induced photothermal PCR cycles. The MEDIC‐PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID‐19 and 103 non‐COVID‐19 patients testing is completed by the MEDIC‐PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC‐PCR can contribute to the preventive global health in the face of a future pandemic. |
| first_indexed | 2024-03-11T19:22:04Z |
| format | Article |
| id | doaj.art-5acb35c5a226485bbfc7b2fcd5eee16e |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-11T19:22:04Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-5acb35c5a226485bbfc7b2fcd5eee16e2023-10-07T03:51:49ZengWileyAdvanced Science2198-38442023-10-011028n/an/a10.1002/advs.202302072Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCRKiran Shrestha0Seongryeong Kim1Jiyeon Han2Gabriela Morales Florez3Han Truong4Trung Hoang5Sajjan Parajuli6Tiara AM7Beomsoo Kim8Younsu Jung9Abdurhaman Teyib Abafogi10Yugyeong Lee11Seung Hyun Song12Jinkee Lee13Sungsu Park14Minhee Kang15Hee Jae Huh16Gyoujin Cho17Luke P. Lee18Department of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biological Science College of Science Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Intelligent Precision Healthcare Convergence Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaSchool of Electronic and Electrical Engineering Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaSchool of Mechanical Engineering Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biomedical Engineering Sungkyunkwan University Suwon 16419 South KoreaDepartment of Electronics Engineering Sookmyung Women's University Seoul 04310 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaBiomedical Engineering Research Center Smart Healthcare Research Institute Samsung Medical Center Seoul 06352 South KoreaSchool of Medicine Department of Laboratory Medicine and Genetics Samsung Medical Center Sungkyunkwan University Seoul 06351 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaDepartment of Biophysics Institute of Quantum Biology Sungkyunkwan University Suwon 16419 South KoreaAbstract The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on‐chip ‐RT‐quantitative PCR (RT‐qPCR): MEDIC‐PCR. First, the authors use a roll‐to‐roll printing process to accomplish low‐cost carbon‐black‐based disposable PCR chips that enable rapid LED‐induced photothermal PCR cycles. The MEDIC‐PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID‐19 and 103 non‐COVID‐19 patients testing is completed by the MEDIC‐PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC‐PCR can contribute to the preventive global health in the face of a future pandemic.https://doi.org/10.1002/advs.202302072covid‐19diagnosticslow‐costrapidrt‐qpcr |
| spellingShingle | Kiran Shrestha Seongryeong Kim Jiyeon Han Gabriela Morales Florez Han Truong Trung Hoang Sajjan Parajuli Tiara AM Beomsoo Kim Younsu Jung Abdurhaman Teyib Abafogi Yugyeong Lee Seung Hyun Song Jinkee Lee Sungsu Park Minhee Kang Hee Jae Huh Gyoujin Cho Luke P. Lee Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR Advanced Science covid‐19 diagnostics low‐cost rapid rt‐qpcr |
| title | Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR |
| title_full | Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR |
| title_fullStr | Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR |
| title_full_unstemmed | Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR |
| title_short | Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR |
| title_sort | mobile efficient diagnostics of infectious diseases via on chip rt qpcr medic pcr |
| topic | covid‐19 diagnostics low‐cost rapid rt‐qpcr |
| url | https://doi.org/10.1002/advs.202302072 |
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