Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding
Kinesin-5 motor consists of two pairs of heads and tail domains, which are situated at the opposite ends of a common stalk. The two pairs of heads can bind to two antiparallel microtubules (MTs) and move on the two MTs independently towards the plus ends, sliding apart the two MTs, which is responsi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/15/7857 |
| _version_ | 1797525578211917824 |
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| author | Yuying Liu Yao Wang Pengye Wang Ping Xie |
| author_facet | Yuying Liu Yao Wang Pengye Wang Ping Xie |
| author_sort | Yuying Liu |
| collection | DOAJ |
| description | Kinesin-5 motor consists of two pairs of heads and tail domains, which are situated at the opposite ends of a common stalk. The two pairs of heads can bind to two antiparallel microtubules (MTs) and move on the two MTs independently towards the plus ends, sliding apart the two MTs, which is responsible for chromosome segregation during mitosis. Prior experimental data showed that the tails of kinesin-5 Eg5 can modulate the dynamics of single motors and are critical for multiple motors to generate high steady forces to slide apart two antiparallel MTs. To understand the molecular mechanism of the tails modulating the ability of Eg5 motors, based on our proposed model the dynamics of the single Eg5 with the tails and that without the tails moving on single MTs is studied analytically and compared. Furthermore, the dynamics of antiparallel MT sliding by multiple Eg5 motors with the tails and that without the tails is studied numerically and compared. Both the analytical results for single motors and the numerical results for multiple motors are consistent with the available experimental data. |
| first_indexed | 2024-03-10T09:14:54Z |
| format | Article |
| id | doaj.art-5ad1ea3e99d94c759532cd17dccc93c8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:14:54Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5ad1ea3e99d94c759532cd17dccc93c82023-11-22T05:39:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012215785710.3390/ijms22157857Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule SlidingYuying Liu0Yao Wang1Pengye Wang2Ping Xie3College of Science, China Agricultural University, Beijing 100083, ChinaCollege of Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, ChinaKey Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, ChinaKinesin-5 motor consists of two pairs of heads and tail domains, which are situated at the opposite ends of a common stalk. The two pairs of heads can bind to two antiparallel microtubules (MTs) and move on the two MTs independently towards the plus ends, sliding apart the two MTs, which is responsible for chromosome segregation during mitosis. Prior experimental data showed that the tails of kinesin-5 Eg5 can modulate the dynamics of single motors and are critical for multiple motors to generate high steady forces to slide apart two antiparallel MTs. To understand the molecular mechanism of the tails modulating the ability of Eg5 motors, based on our proposed model the dynamics of the single Eg5 with the tails and that without the tails moving on single MTs is studied analytically and compared. Furthermore, the dynamics of antiparallel MT sliding by multiple Eg5 motors with the tails and that without the tails is studied numerically and compared. Both the analytical results for single motors and the numerical results for multiple motors are consistent with the available experimental data.https://www.mdpi.com/1422-0067/22/15/7857kinesin-5antiparallel microtubule slidingtail domainmolecular motorcatch-bond |
| spellingShingle | Yuying Liu Yao Wang Pengye Wang Ping Xie Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding International Journal of Molecular Sciences kinesin-5 antiparallel microtubule sliding tail domain molecular motor catch-bond |
| title | Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding |
| title_full | Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding |
| title_fullStr | Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding |
| title_full_unstemmed | Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding |
| title_short | Effect of Kinesin-5 Tail Domain on Motor Dynamics for Antiparallel Microtubule Sliding |
| title_sort | effect of kinesin 5 tail domain on motor dynamics for antiparallel microtubule sliding |
| topic | kinesin-5 antiparallel microtubule sliding tail domain molecular motor catch-bond |
| url | https://www.mdpi.com/1422-0067/22/15/7857 |
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