The role of podoplanin in the biology of differentiated thyroid cancers.
Podoplanin (PDPN), a mucin-type transmembrane glycoprotein specific to the lymphatic system is expressed in a variety of human cancers, and is regarded as a factor promoting tumor progression. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cell...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4010536?pdf=render |
| _version_ | 1811235409037885440 |
|---|---|
| author | Magdalena Rudzińska Damian Gaweł Justyna Sikorska Kamila M Karpińska Mirosław Kiedrowski Tomasz Stępień Magdalena Marchlewska Barbara Czarnocka |
| author_facet | Magdalena Rudzińska Damian Gaweł Justyna Sikorska Kamila M Karpińska Mirosław Kiedrowski Tomasz Stępień Magdalena Marchlewska Barbara Czarnocka |
| author_sort | Magdalena Rudzińska |
| collection | DOAJ |
| description | Podoplanin (PDPN), a mucin-type transmembrane glycoprotein specific to the lymphatic system is expressed in a variety of human cancers, and is regarded as a factor promoting tumor progression. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells. PDPN expression was evaluated in primary thyroid carcinomas and thyroid carcinoma cell lines by RT-qPCR, Western blotting, IF and IHC. To examine the role of podoplanin in determining a cell's malignant potential (cellular migration, invasion, proliferation, adhesion, motility, apoptosis), a thyroid cancer cell line with silenced PDPN expression was used. We observed that PDPN was solely expressed in the cancer cells of 40% of papillary thyroid carcinoma (PTC) tissues. Moreover, PDPN mRNA and protein were highly expressed in PTC-derived TPC1 and BcPAP cell lines but were not detected in follicular thyroid cancer derived cell lines. PDPN knock-down significantly decreased cellular invasion, and modestly reduced cell migration, while proliferation and adhesion were not affected. Our results demonstrate that PDPN mediates the invasive properties of cells derived from papillary thyroid carcinomas, suggesting that podoplanin might promote PTC progression. |
| first_indexed | 2024-04-12T11:51:29Z |
| format | Article |
| id | doaj.art-5ad1f4fc1d0346f48f54b6ddd56f197d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:51:29Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5ad1f4fc1d0346f48f54b6ddd56f197d2022-12-22T03:34:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9654110.1371/journal.pone.0096541The role of podoplanin in the biology of differentiated thyroid cancers.Magdalena RudzińskaDamian GawełJustyna SikorskaKamila M KarpińskaMirosław KiedrowskiTomasz StępieńMagdalena MarchlewskaBarbara CzarnockaPodoplanin (PDPN), a mucin-type transmembrane glycoprotein specific to the lymphatic system is expressed in a variety of human cancers, and is regarded as a factor promoting tumor progression. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells. PDPN expression was evaluated in primary thyroid carcinomas and thyroid carcinoma cell lines by RT-qPCR, Western blotting, IF and IHC. To examine the role of podoplanin in determining a cell's malignant potential (cellular migration, invasion, proliferation, adhesion, motility, apoptosis), a thyroid cancer cell line with silenced PDPN expression was used. We observed that PDPN was solely expressed in the cancer cells of 40% of papillary thyroid carcinoma (PTC) tissues. Moreover, PDPN mRNA and protein were highly expressed in PTC-derived TPC1 and BcPAP cell lines but were not detected in follicular thyroid cancer derived cell lines. PDPN knock-down significantly decreased cellular invasion, and modestly reduced cell migration, while proliferation and adhesion were not affected. Our results demonstrate that PDPN mediates the invasive properties of cells derived from papillary thyroid carcinomas, suggesting that podoplanin might promote PTC progression.http://europepmc.org/articles/PMC4010536?pdf=render |
| spellingShingle | Magdalena Rudzińska Damian Gaweł Justyna Sikorska Kamila M Karpińska Mirosław Kiedrowski Tomasz Stępień Magdalena Marchlewska Barbara Czarnocka The role of podoplanin in the biology of differentiated thyroid cancers. PLoS ONE |
| title | The role of podoplanin in the biology of differentiated thyroid cancers. |
| title_full | The role of podoplanin in the biology of differentiated thyroid cancers. |
| title_fullStr | The role of podoplanin in the biology of differentiated thyroid cancers. |
| title_full_unstemmed | The role of podoplanin in the biology of differentiated thyroid cancers. |
| title_short | The role of podoplanin in the biology of differentiated thyroid cancers. |
| title_sort | role of podoplanin in the biology of differentiated thyroid cancers |
| url | http://europepmc.org/articles/PMC4010536?pdf=render |
| work_keys_str_mv | AT magdalenarudzinska theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT damiangaweł theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT justynasikorska theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT kamilamkarpinska theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT mirosławkiedrowski theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT tomaszstepien theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT magdalenamarchlewska theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT barbaraczarnocka theroleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT magdalenarudzinska roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT damiangaweł roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT justynasikorska roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT kamilamkarpinska roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT mirosławkiedrowski roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT tomaszstepien roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT magdalenamarchlewska roleofpodoplanininthebiologyofdifferentiatedthyroidcancers AT barbaraczarnocka roleofpodoplanininthebiologyofdifferentiatedthyroidcancers |