The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts
Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is a multifunctional protein essential for basic replication of CaMV. It also plays a role in viral pathogenesis in crucifer and solanaceous host plants. Deletion mutagenesis revealed that N- and C-terminal parts of Tav are not essenti...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2004-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.5.475 |
| _version_ | 1811275701282668544 |
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| author | Kappei Kobayashi Thomas Hohn |
| author_facet | Kappei Kobayashi Thomas Hohn |
| author_sort | Kappei Kobayashi |
| collection | DOAJ |
| description | Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is a multifunctional protein essential for basic replication of CaMV. It also plays a role in viral pathogenesis in crucifer and solanaceous host plants. Deletion mutagenesis revealed that N- and C-terminal parts of Tav are not essential for CaMV replication in transfected protoplasts. Two deletion mutants having only minimal defects in basic replication were infectious in turnips but only with highly attenuated virulence. This was shown to be due to delayed virus spread within the inoculated leaves and to the upper leaves. Unlike the wild-type virus, the mutant viruses successfully spread locally without inducing a host defense response in inoculated Datura stramonium leaves, but did not spread systemically. These results provide the first evidence that a Tav domain required for avirulence function in solanaceous plants is not essential for CaMV infectivity but has a role in viral virulence in susceptible hosts. |
| first_indexed | 2024-04-12T23:42:28Z |
| format | Article |
| id | doaj.art-5ad2b1ca183446de9759b81733dd2d9a |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T23:42:28Z |
| publishDate | 2004-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-5ad2b1ca183446de9759b81733dd2d9a2022-12-22T03:11:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-05-0117547548310.1094/MPMI.2004.17.5.475The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible HostsKappei KobayashiThomas HohnCauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is a multifunctional protein essential for basic replication of CaMV. It also plays a role in viral pathogenesis in crucifer and solanaceous host plants. Deletion mutagenesis revealed that N- and C-terminal parts of Tav are not essential for CaMV replication in transfected protoplasts. Two deletion mutants having only minimal defects in basic replication were infectious in turnips but only with highly attenuated virulence. This was shown to be due to delayed virus spread within the inoculated leaves and to the upper leaves. Unlike the wild-type virus, the mutant viruses successfully spread locally without inducing a host defense response in inoculated Datura stramonium leaves, but did not spread systemically. These results provide the first evidence that a Tav domain required for avirulence function in solanaceous plants is not essential for CaMV infectivity but has a role in viral virulence in susceptible hosts.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.5.475guard modelvirulence factor |
| spellingShingle | Kappei Kobayashi Thomas Hohn The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts Molecular Plant-Microbe Interactions guard model virulence factor |
| title | The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts |
| title_full | The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts |
| title_fullStr | The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts |
| title_full_unstemmed | The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts |
| title_short | The Avirulence Domain of Cauliflower mosaic virus Transactivator/Viroplasmin Is a Determinant of Viral Virulence in Susceptible Hosts |
| title_sort | avirulence domain of cauliflower mosaic virus transactivator viroplasmin is a determinant of viral virulence in susceptible hosts |
| topic | guard model virulence factor |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.5.475 |
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