Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold
Composite scaffolds obtained by the combination of biodegradable porous scaffolds and hydroxyapatite with bone regeneration potential are feasible materials for bone tissue engineering. However, most composite scaffolds have been fabricated by complicated procedures or under thermally harsh conditio...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9048 |
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| author | Ye Zhang Jun-Ichiro Jo Liji Chen Shigeki Hontsu Yoshiya Hashimoto |
| author_facet | Ye Zhang Jun-Ichiro Jo Liji Chen Shigeki Hontsu Yoshiya Hashimoto |
| author_sort | Ye Zhang |
| collection | DOAJ |
| description | Composite scaffolds obtained by the combination of biodegradable porous scaffolds and hydroxyapatite with bone regeneration potential are feasible materials for bone tissue engineering. However, most composite scaffolds have been fabricated by complicated procedures or under thermally harsh conditions. We have previously demonstrated that hydroxyapatite coating onto various substrates under a thermally mild condition was achieved by erbium-doped yttrium aluminum garnet (Er: YAG) pulsed laser deposition (PLD). The purpose of this study was to prepare a polycaprolactone (PCL) porous scaffold coated with the hydroxyapatite by the Er: YAG-PLD method. Hydroxyapatite coating by the Er: YAG-PLD method was confirmed by morphology, crystallographic analysis, and surface chemical characterization studies. When cultured on PCL porous scaffold coated with hydroxyapatite, rat bone marrow-derived mesenchymal stem cells adhered, spread, and proliferated well. The micro-CT and staining analyses after the implantation of scaffold into the critical-sized calvaria bone defect in rats indicate that PCL porous scaffold coated with hydroxyapatite demonstrates accelerated and widespread bone formation. In conclusion, PCL porous scaffold coated with hydroxyapatite obtained by the Er: YAG-PLD method is a promising material in bone tissue engineering. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:17:24Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-5ad2bb52643342fabab1df6190aefa732023-11-30T21:33:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316904810.3390/ijms23169048Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous ScaffoldYe Zhang0Jun-Ichiro Jo1Liji Chen2Shigeki Hontsu3Yoshiya Hashimoto4Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata-shi, Osaka 573-1121, JapanDepartment of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata-shi, Osaka 573-1121, JapanDepartment of Orthodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata-shi, Osaka 573-1121, JapanDepartment of Biomedical Engineering, Faculty of Biology-Oriented Science and Technology, Kindai University, 930 Nishimitani, Kinokawa-shi, Wakayama 649-6493, JapanDepartment of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata-shi, Osaka 573-1121, JapanComposite scaffolds obtained by the combination of biodegradable porous scaffolds and hydroxyapatite with bone regeneration potential are feasible materials for bone tissue engineering. However, most composite scaffolds have been fabricated by complicated procedures or under thermally harsh conditions. We have previously demonstrated that hydroxyapatite coating onto various substrates under a thermally mild condition was achieved by erbium-doped yttrium aluminum garnet (Er: YAG) pulsed laser deposition (PLD). The purpose of this study was to prepare a polycaprolactone (PCL) porous scaffold coated with the hydroxyapatite by the Er: YAG-PLD method. Hydroxyapatite coating by the Er: YAG-PLD method was confirmed by morphology, crystallographic analysis, and surface chemical characterization studies. When cultured on PCL porous scaffold coated with hydroxyapatite, rat bone marrow-derived mesenchymal stem cells adhered, spread, and proliferated well. The micro-CT and staining analyses after the implantation of scaffold into the critical-sized calvaria bone defect in rats indicate that PCL porous scaffold coated with hydroxyapatite demonstrates accelerated and widespread bone formation. In conclusion, PCL porous scaffold coated with hydroxyapatite obtained by the Er: YAG-PLD method is a promising material in bone tissue engineering.https://www.mdpi.com/1422-0067/23/16/9048hydroxyapatite coatingEr: YAG laserpulsed laser depositionpolycaprolactoneporous scaffoldbone formation |
| spellingShingle | Ye Zhang Jun-Ichiro Jo Liji Chen Shigeki Hontsu Yoshiya Hashimoto Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold International Journal of Molecular Sciences hydroxyapatite coating Er: YAG laser pulsed laser deposition polycaprolactone porous scaffold bone formation |
| title | Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold |
| title_full | Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold |
| title_fullStr | Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold |
| title_full_unstemmed | Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold |
| title_short | Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold |
| title_sort | effect of hydroxyapatite coating by er yag pulsed laser deposition on the bone formation efficacy by polycaprolactone porous scaffold |
| topic | hydroxyapatite coating Er: YAG laser pulsed laser deposition polycaprolactone porous scaffold bone formation |
| url | https://www.mdpi.com/1422-0067/23/16/9048 |
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