Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon
<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>antigenic diversity and polymorphism confuses the issue of antimalarial vaccine development. Merozoite surface protein (MSP)-1 and -2 are two highly polymorphic vaccine candidates. Character...
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| Format: | Article |
| Language: | English |
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BMC
2003-05-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/2/1/12 |
| _version_ | 1818682873023037440 |
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| author | Deloron Philippe Aubouy Agnès Migot-Nabias Florence |
| author_facet | Deloron Philippe Aubouy Agnès Migot-Nabias Florence |
| author_sort | Deloron Philippe |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>antigenic diversity and polymorphism confuses the issue of antimalarial vaccine development. Merozoite surface protein (MSP)-1 and -2 are two highly polymorphic vaccine candidates. Characterisation of their precise polymorphism in endemic regions may facilitate the design of an effective vaccine.</p> <p>Methods</p> <p>Isolates obtained in 52 Gabonese children presenting with uncomplicated malaria were genotyped by nested-PCR of <it>msp</it>-1 block 2, and <it>msp</it>-2 block 3, to analyze both parasite population polymorphism and clone fluctuations.</p> <p>Results</p> <p>Twenty-five and 19 different alleles were respectively obtained for <it>msp-1 </it>and <it>msp-2 </it>loci, the RO33 family of <it>msp-1 </it>being poorly polymorphic. Four cases of non-random distribution of alleles were reported of the FC27, and/or 3D7 families of <it>msp-2</it>. All but two isolates were composed of more than one genotype, and the multiplicity of infection (MOI) was 4.0. Neither parasite density nor age was related to MOI. Clone fluctuations were studied for ten subjects who were sampled again at reappearance of parasites in blood. Disappearance and reappearance of alleles were observed following treatment, suggesting difficulties in assessing polymorphism and in distinguishing reinfection from recrudescence.</p> <p>Conclusion</p> <p><it>P. falciparum </it>polymorphism is extensive in Southeast Gabon, and most of infections are composed of multiple clones. The fluctuation of clones contributes to parasite diversity.</p> |
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| format | Article |
| id | doaj.art-5ad39fbd00ec4adcbec37a691dfd903f |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-17T10:25:45Z |
| publishDate | 2003-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-5ad39fbd00ec4adcbec37a691dfd903f2022-12-21T21:52:40ZengBMCMalaria Journal1475-28752003-05-01211210.1186/1475-2875-2-12Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from GabonDeloron PhilippeAubouy AgnèsMigot-Nabias Florence<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>antigenic diversity and polymorphism confuses the issue of antimalarial vaccine development. Merozoite surface protein (MSP)-1 and -2 are two highly polymorphic vaccine candidates. Characterisation of their precise polymorphism in endemic regions may facilitate the design of an effective vaccine.</p> <p>Methods</p> <p>Isolates obtained in 52 Gabonese children presenting with uncomplicated malaria were genotyped by nested-PCR of <it>msp</it>-1 block 2, and <it>msp</it>-2 block 3, to analyze both parasite population polymorphism and clone fluctuations.</p> <p>Results</p> <p>Twenty-five and 19 different alleles were respectively obtained for <it>msp-1 </it>and <it>msp-2 </it>loci, the RO33 family of <it>msp-1 </it>being poorly polymorphic. Four cases of non-random distribution of alleles were reported of the FC27, and/or 3D7 families of <it>msp-2</it>. All but two isolates were composed of more than one genotype, and the multiplicity of infection (MOI) was 4.0. Neither parasite density nor age was related to MOI. Clone fluctuations were studied for ten subjects who were sampled again at reappearance of parasites in blood. Disappearance and reappearance of alleles were observed following treatment, suggesting difficulties in assessing polymorphism and in distinguishing reinfection from recrudescence.</p> <p>Conclusion</p> <p><it>P. falciparum </it>polymorphism is extensive in Southeast Gabon, and most of infections are composed of multiple clones. The fluctuation of clones contributes to parasite diversity.</p>http://www.malariajournal.com/content/2/1/12 |
| spellingShingle | Deloron Philippe Aubouy Agnès Migot-Nabias Florence Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon Malaria Journal |
| title | Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon |
| title_full | Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon |
| title_fullStr | Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon |
| title_full_unstemmed | Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon |
| title_short | Polymorphism in two merozoite surface proteins of <it>Plasmodium falciparum </it>isolates from Gabon |
| title_sort | polymorphism in two merozoite surface proteins of it plasmodium falciparum it isolates from gabon |
| url | http://www.malariajournal.com/content/2/1/12 |
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