Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats
E’Jiao is a traditional Chinese medicine derived from donkey skin. E’Jiao is reported to suppress elevated bone remodelling in ovariectomised rats but its mechanism of action is not known. To bridge this research gap, the current study aims to investigate the effects of E’Jiao on skeletal mineralisa...
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MDPI AG
2023-02-01
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| author | Kok-Yong Chin Ben Nett Ng Muhd Khairik Imran Rostam Nur Farah Dhaniyah Muhammad Fadzil Vaishnavi Raman Farzana Mohamed Yunus Wun Fui Mark-Lee Yan Yi Chong Jing Qian Yan Zhang Haibin Qu Syed Alhafiz Syed Hashim Sophia Ogechi Ekeuku |
| author_facet | Kok-Yong Chin Ben Nett Ng Muhd Khairik Imran Rostam Nur Farah Dhaniyah Muhammad Fadzil Vaishnavi Raman Farzana Mohamed Yunus Wun Fui Mark-Lee Yan Yi Chong Jing Qian Yan Zhang Haibin Qu Syed Alhafiz Syed Hashim Sophia Ogechi Ekeuku |
| author_sort | Kok-Yong Chin |
| collection | DOAJ |
| description | E’Jiao is a traditional Chinese medicine derived from donkey skin. E’Jiao is reported to suppress elevated bone remodelling in ovariectomised rats but its mechanism of action is not known. To bridge this research gap, the current study aims to investigate the effects of E’Jiao on skeletal mineralisation, osteocyte and WNT signalling inhibitors in ovariectomised rats. Female Sprague–Dawley rats (3 months old) were ovariectomised and supplemented with E’Jiao at 0.26 g/kg, 0.53 g/kg and 1.06 g/kg, or 1% calcium carbonate (<i>w</i>/<i>v</i>) in drinking water. The rats were euthanised after two months of supplementation and their bones were collected for Fourier-transform infrared spectroscopy, histomorphometry and protein analysis. Neither ovariectomy nor treatment affected the skeletal mineral/matrix ratio, osteocyte number, empty lacunar number, and Dickkopf-1 and sclerostin protein levels (<i>p</i> > 0.05). Rats treated with calcium carbonate had a higher Dickkopf-1 level than baseline (<i>p</i> = 0.002) and E’Jiao at 0.53 g/kg (<i>p</i> = 0.002). In conclusion, E’Jiao has no significant effect on skeletal mineralisation, osteocyte and WNT signalling inhibitors in ovariectomised rats. The skeletal effect of E’Jiao might not be mediated through osteocytes. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T08:32:25Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-5ad6c1c296db479e9889f03720ffba882023-11-16T21:43:05ZengMDPI AGLife2075-17292023-02-0113257010.3390/life13020570Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised RatsKok-Yong Chin0Ben Nett Ng1Muhd Khairik Imran Rostam2Nur Farah Dhaniyah Muhammad Fadzil3Vaishnavi Raman4Farzana Mohamed Yunus5Wun Fui Mark-Lee6Yan Yi Chong7Jing Qian8Yan Zhang9Haibin Qu10Syed Alhafiz Syed Hashim11Sophia Ogechi Ekeuku12Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Chemistry, Faculty of Science, University Teknologi Malaysia (UTM), Johor Bahru 81310, MalaysiaSchool of Pre-University Studies, Taylor’s College, Subang Jaya 47500, MalaysiaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310030, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310030, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310030, ChinaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras 56000, MalaysiaE’Jiao is a traditional Chinese medicine derived from donkey skin. E’Jiao is reported to suppress elevated bone remodelling in ovariectomised rats but its mechanism of action is not known. To bridge this research gap, the current study aims to investigate the effects of E’Jiao on skeletal mineralisation, osteocyte and WNT signalling inhibitors in ovariectomised rats. Female Sprague–Dawley rats (3 months old) were ovariectomised and supplemented with E’Jiao at 0.26 g/kg, 0.53 g/kg and 1.06 g/kg, or 1% calcium carbonate (<i>w</i>/<i>v</i>) in drinking water. The rats were euthanised after two months of supplementation and their bones were collected for Fourier-transform infrared spectroscopy, histomorphometry and protein analysis. Neither ovariectomy nor treatment affected the skeletal mineral/matrix ratio, osteocyte number, empty lacunar number, and Dickkopf-1 and sclerostin protein levels (<i>p</i> > 0.05). Rats treated with calcium carbonate had a higher Dickkopf-1 level than baseline (<i>p</i> = 0.002) and E’Jiao at 0.53 g/kg (<i>p</i> = 0.002). In conclusion, E’Jiao has no significant effect on skeletal mineralisation, osteocyte and WNT signalling inhibitors in ovariectomised rats. The skeletal effect of E’Jiao might not be mediated through osteocytes.https://www.mdpi.com/2075-1729/13/2/570Colla corii asiniDickkopf-1sclerostinparathyroid hormoneosteoporosis |
| spellingShingle | Kok-Yong Chin Ben Nett Ng Muhd Khairik Imran Rostam Nur Farah Dhaniyah Muhammad Fadzil Vaishnavi Raman Farzana Mohamed Yunus Wun Fui Mark-Lee Yan Yi Chong Jing Qian Yan Zhang Haibin Qu Syed Alhafiz Syed Hashim Sophia Ogechi Ekeuku Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats Life Colla corii asini Dickkopf-1 sclerostin parathyroid hormone osteoporosis |
| title | Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats |
| title_full | Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats |
| title_fullStr | Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats |
| title_full_unstemmed | Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats |
| title_short | Effects of E’Jiao on Skeletal Mineralisation, Osteocyte and WNT Signalling Inhibitors in Ovariectomised Rats |
| title_sort | effects of e jiao on skeletal mineralisation osteocyte and wnt signalling inhibitors in ovariectomised rats |
| topic | Colla corii asini Dickkopf-1 sclerostin parathyroid hormone osteoporosis |
| url | https://www.mdpi.com/2075-1729/13/2/570 |
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