Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes
Potato is one of the most important food crops in the world and also in the Russian Federation. Among harmful organisms reducing potato yield potential, the potato cyst nematodes (PCN) are considered to be ones of the most damaging pests. Information on PCN resistant cultivars is important for potat...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/12/2400 |
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| author | Tatjana A. Gavrilenko Aleksander V. Khiutti Natalia S. Klimenko Olga Y. Antonova Natalia A. Fomina Olga S. Afanasenko |
| author_facet | Tatjana A. Gavrilenko Aleksander V. Khiutti Natalia S. Klimenko Olga Y. Antonova Natalia A. Fomina Olga S. Afanasenko |
| author_sort | Tatjana A. Gavrilenko |
| collection | DOAJ |
| description | Potato is one of the most important food crops in the world and also in the Russian Federation. Among harmful organisms reducing potato yield potential, the potato cyst nematodes (PCN) are considered to be ones of the most damaging pests. Information on PCN resistant cultivars is important for potato breeding and production. Russian potato cultivars are characterized in the state-bio-test program for resistance to only one PCN species <i>Globodera rostochiensis</i> and one pathotype Ro1 which is reported to be present in the country. This study aimed to find domestic cultivars with multiple resistances to different PCN species and different pathotypes using phenotyping coupled with molecular marker analysis due to the risk of the occasional introduction of new pests. The phenotypic response was determined by the inoculation of plants with pathotypes Ro5 of <i>G. rostochiensis</i> and Pa3 of <i>G. pallida</i>. The obtained results were supplemented by the state-bio-test data on resistance to Ro1 of <i>G. rostochiensis</i>. Nine of 26 Russian cultivars were resistant both to Ro5 and Ro1 pathotypes and two cultivars possess multiple resistances to both PCN species. Most tested molecular markers associated with the <i>Gpa2</i>, <i>GpaV<sub>vrn</sub></i>, <i>GpaV<sup>s</sup><sub>spl</sub></i>, <i>Grp1</i> loci showed discrepancies with phenotyping. However, a predictive haplotype and epistatic effect were detected. |
| first_indexed | 2024-03-10T04:41:28Z |
| format | Article |
| id | doaj.art-5adb5b59a8b7496fa7b0be8eef35cf93 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T04:41:28Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-5adb5b59a8b7496fa7b0be8eef35cf932023-11-23T03:21:16ZengMDPI AGAgronomy2073-43952021-11-011112240010.3390/agronomy11122400Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst NematodesTatjana A. Gavrilenko0Aleksander V. Khiutti1Natalia S. Klimenko2Olga Y. Antonova3Natalia A. Fomina4Olga S. Afanasenko5N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint-Petersburg, RussiaAll Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 Saint-Petersburg, RussiaAll Russian Institute of Plant Protection (FSBSI VIZR), 196608 Saint-Petersburg, RussiaPotato is one of the most important food crops in the world and also in the Russian Federation. Among harmful organisms reducing potato yield potential, the potato cyst nematodes (PCN) are considered to be ones of the most damaging pests. Information on PCN resistant cultivars is important for potato breeding and production. Russian potato cultivars are characterized in the state-bio-test program for resistance to only one PCN species <i>Globodera rostochiensis</i> and one pathotype Ro1 which is reported to be present in the country. This study aimed to find domestic cultivars with multiple resistances to different PCN species and different pathotypes using phenotyping coupled with molecular marker analysis due to the risk of the occasional introduction of new pests. The phenotypic response was determined by the inoculation of plants with pathotypes Ro5 of <i>G. rostochiensis</i> and Pa3 of <i>G. pallida</i>. The obtained results were supplemented by the state-bio-test data on resistance to Ro1 of <i>G. rostochiensis</i>. Nine of 26 Russian cultivars were resistant both to Ro5 and Ro1 pathotypes and two cultivars possess multiple resistances to both PCN species. Most tested molecular markers associated with the <i>Gpa2</i>, <i>GpaV<sub>vrn</sub></i>, <i>GpaV<sup>s</sup><sub>spl</sub></i>, <i>Grp1</i> loci showed discrepancies with phenotyping. However, a predictive haplotype and epistatic effect were detected.https://www.mdpi.com/2073-4395/11/12/2400potato (<i>Solanum tuberosum</i>)nematode resistance<i>Globodera rostochiensis</i><i>Globodera pallida</i>bioassaysmarker-assisted selection |
| spellingShingle | Tatjana A. Gavrilenko Aleksander V. Khiutti Natalia S. Klimenko Olga Y. Antonova Natalia A. Fomina Olga S. Afanasenko Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes Agronomy potato (<i>Solanum tuberosum</i>) nematode resistance <i>Globodera rostochiensis</i> <i>Globodera pallida</i> bioassays marker-assisted selection |
| title | Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes |
| title_full | Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes |
| title_fullStr | Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes |
| title_full_unstemmed | Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes |
| title_short | Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes |
| title_sort | phenotypic and dna marker assisted characterization of russian potato cultivars for resistance to potato cyst nematodes |
| topic | potato (<i>Solanum tuberosum</i>) nematode resistance <i>Globodera rostochiensis</i> <i>Globodera pallida</i> bioassays marker-assisted selection |
| url | https://www.mdpi.com/2073-4395/11/12/2400 |
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